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A bacterial strain, designated GEM5(T), was isolated from sand soil samples from the Qinghai-Tibet Plateau. The polyphasic study confirmed the affiliation of the isolate with the genus Massilia. GEM5(T) had Gram-stain-negative, non-spore-forming and rod-shaped cells and grew at 4-30 degrees C. pH 6-8 and with 0-2% (w/v) NaCl. Its cell wall contained ribose. Q8 was the predominant respiratory quinone, and summed feature 3 (C-1(6:1), omega 6c/w7c) and C-16:0 were the major components of the fatty acids. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified phospholipid, an unidentified aminolipid and four unidentified lipids. The DNA G+C content was 65.1 mol%. The phylogenetic analysis based on the 16S rRNA gene showed a stable Glade being formed by GEM5(T), Massilia timonae CCUG 45783(T) (97.94%) and Massilia oculi CCUG 43427A(T) (97.58%). The average nucleotide identity (ANIb) values between GEM5(T) and M. timonae CCUG 45783(T), M.oculi CCUG 43427A(T) were 91.3 and 91.7%, respectively. On the basis of the morphological, physiological and chemotaxonomic pattern, it was proposed that strain GEM5(T) (=JCM 32744(T)=CICC 24458(T)) should be classified as representing a member of the genus Massilia with the name Massilia arenae sp. nov.

来源平台：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY