From the point of view of the Earth System Science,CO2,as the important driving force of karstification, always ran through the open three-phase CaCO3 (solid)-CO2 (gas)-H2O (liquid) unbalanced system. In the relatively stable environment of karst cave, there are some scientific questions that are emergent to be researched and resolved including, how CO2 in the cave system respond to external changes in the atmospheric environment and how cave climate effects the carbonate depositional environment. The typical karst cave in Guizhou Province is selected as the study area in the scientific program. Infrared remote sensing, water chemistry analysis and isotope tracer techniques are chosen for using comprehensively to study the CO2 change rule and migration process within the cave system.Comparison of CO2 concentration variation in karst cave system inside and outside, coupled climate and environment change, to reveal the response mechanism of the internal CO2 changes in the external environment. Through the analysis of fixed-point observation and experiment, coupled karst cave climate and environment, the mechanism of the impact of climatic and environmental changes in the caves of carbonate depositional environments will be clarified, and the cave landscape formation and developmental mechanisms will be reveal, the study will provide theory bases for assessed the impact of atmospheric environment changes in the karst caves.