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Forest soil is crucial in climate change mitigation, food security, and biogeochemical nutrient cycling. Mixed Sal forests enhance soil organic matter, improve nutrient availability, and regulate pH dynamics. However, anthropogenic disturbances, including deforestation and land-use changes, significantly alter forest cover, leading to shifts in soil physicochemical and microbial properties. These impacts necessitate rigorous monitoring and comprehensive assessment. Therefore, we investigated the effects of contrasting conditions- closed (no human activities) and open (human interferences) mixed Sal Forest on the vertical and seasonal dynamics of microbial biomass carbon (SMBC). Results revealed that the closed mixed Sal Forest had significantly higher SMBC than the open mixed Sal Forest across the soil profile (D1-D5) with a strong seasonal effect. Closed mixed Sal Forest had 60% higher SMBC in D1 than open mixed Sal Forest while it reduced with depth and 17.1 to 56.7% higher SMBC in the subsurface to bottom-most soil profile (D2-D5). Moreover, SMBC was higher in the monsoon period in both forests. The SMBC reduced by 24.2 to 45.1% in the post-monsoon period while reduction was more intense in the pre-monsoon period (48.1 to 68.2%) compared to the monsoon period under closed mixed Sal Forest. Similarly, the decline was more intense in the open mixed Sal Forest, where SMBC declined 12.1 to 54% in the post-monsoon period and 56.1 to 76.2% in the pre-monsoon period compared to the monsoon period. The study indicates that human interference in mixed Sal forests leads to loss of forest cover, negatively affecting microbiological properties and reducing soil fertility, which weakens the forest's resilience to climate change. Additionally, SMBC exhibits seasonal variations, reflecting responses to environmental conditions. These results underline the need to reduce human disturbances and enhance forest conservation strategies to ensure soil sustainability and ecosystem stability.

来源平台：SCIENTIFIC REPORTS