The impact of global climate change and human-induced nitrogen (N) deposition on winter weather patterns will have consequences for soil N cycling and greenhouse gas emissions in temperate deserts. Biological soil crusts (referred to as biocrusts) are crucial communities in soil and significant sources of nitrous oxide (N2O) emission in desert ecosystems and are sensitive to environmental changes. The contribution of bacteria and fungi to N2O production in drylands has been acknowledged. However, the effect of changes in snow cover and N deposition on the N2O production of different microbial groups of microorganisms is not yet clear. In this study, we examine the responses of fungi and bacteria mediated pathways involved in soil N2O production from biocrusts to longterm snow cover manipulation and N addition experiments in the Gurbantunggut Desert. These soils were incubated and subjected to biocide treatments (such as cycloheximide and streptomycin, and fungal and bacterial inhibitors), after which rates of potential nitrification and N2O production were measured. Compared with controls, snow removal treatments from bare sand, lichen crust and moss crust reduced background rates of N2O production by 29.41 %, 26.21 % and 20.49 %, respectively; N2O production rates were 1.53-fold higher in bare sand, 1.38-fold higher in lichen crust, and 1.56-fold higher in moss crust after N addition. The addition of streptomycin significantly reduced the potential nitrification rates of bare sand and biocrusts, indicating that bacteria may be important sources of NO3- production in biocrusts rather than fungi. Conversely, fungi were main sources of N2O production in biocrusts. Additionally, fungi also played a major role in N2O production in biocrusts after snow cover manipulation and N addition. Both snow cover manipulation and N addition treatment indirectly affected the N2O production in biocrusts by considerably affecting the content of substrate N and the abundance of microbial groups. Our research suggests that fungi are main contributors for denitrification in biocrusts, and that snow cover changes (removal snow and double snow) and N addition alter the contribution of biotic pathways responsible for N cycling.

The relationship between soil temperature and its variations with different types of land cover are critical to understanding the effects of climate warming on ecohydrological processes in frozen soil regions such as the Qinghai-Tibet Plateau (QTP) of China. Biological soil crusts (biocrusts), which cover approximately 40% of the open soil surface in frozen soil regions, exert great impacts on soil temperatures. However, little attention has been given to the potential effects of biocrusts on the temperature characteristics, dynamics and freezing duration of soil in frozen soil regions. To provide more insight into this issue, an automatic system was used to monitor soil temperatures and dynamics at depths of 5, 30, 50 and 100 cm beneath bare soil and two types of biocrustal soils (soils covered with two types of biocrusts) on the QTP of China. The results showed that biocrusts play an important role in controlling the dynamics of soil temperatures. Biocrusts cause a 0.6-1 degrees C decrease in the mean annual temperature of soils down to a depth of 100 cm. The extent of the decrease in soil temperature was dependent on biocrust type, and dark biocrust showed a greater reduction in soil temperature than light biocrust. In addition, reductions in soil temperatures of biocrusts mainly occurred in daytimes of the thawing period, and this prolonged the freezing duration in the top 100 cm by approximately 10-20 days. The results of this study indicate that biocrusts maintain lower temperatures in the thawing period and slow the thawing of frozen soil in the spring, which helps to maintain the stability of the frozen soil. This information may aid understanding of the function of biocrusts in the frozen soil regions under global warming conditions.