Abandoned farmlands are increasing due to socio-economic changes and land marginalization, and they require sustainable land management practices. Biocrusts are a common cover on the topsoil of abandoned farmlands and play an important role in improving soil stability and erosion resistance. The critical functions of biocrusts are known to mostly rely on their biofilaments and extracellular polymeric substances (EPS), but how these components act at microscopic scale is still unknown, while rheological methods are able to provide new insights into biocrust microstructural stability at particle scale. Here, bare soil and two representative types of biocrusts (cyanobacterial and moss crusts) developed on sandy (Ustipsamments) and sandy loam (Haplustepts) soils in abandoned farmlands in the northern Chinese Loess Plateau were collected at a sampling depth of 2 cm. Changes in the rheological properties of the biocrusts were analyzed with respect to their biofilament network and EPS contents to provide possible explanations. The rheological results showed that compared with bare soil, storage and loss moduli were decreased by the biocrusts on sandy soil, but they were increased by the biocrusts on sandy loam soil. Other rheological parameters tau max, gamma L, gamma YP, and Iz of biocrusts on both soils were significantly higher than those of bare soil, showing higher viscoelasticity. And the moss crusts had about 10 times higher rheological property values than the cyanobacterial crusts. Analysis from SEM images showed that the moss crusts had higher biofilament network parameters than the cyanobacterial crusts, including nodes, crosslink density, branches, branching ratio and mesh index, and biofilament density, indicating that the biofilament network structure in the moss crusts was more compact and complex in contrast to the cyanobacterial crusts. Additionally, EPS content of the moss crusts was higher than that of the cyanobacterial crusts on both soils. Overall, the crosslink density, biofilament density, and EPS content of the biocrusts were significantly and positively correlated with their gamma YP and Iz. The interaction between crosslink density and biofilament density contributed 73.2 % of gamma YP, and that between crosslink density and EPS content contributed 84.0 % of Iz. Our findings highlight the biocrusts-induced changes of abandoned farmland soil rheological properties in drylands, and the importance of biocrust biofilament network and EPS in maintaining abandoned farmland soil microstructural stability to resist soil water/wind erosion and degradation, providing a new perspective for sustainable management of abandoned farmlands.

Effective erosion mitigation in the Pisha sandstone region is crucial for soil and water conservation in the Yellow River Basin, yet existing vegetation measures are inadequate in water-limited environments. This study examines the application of drought-tolerant biological soil crusts (biocrusts) for erosion control on sandstone slopes and evaluates their erosion-reducing effects under varying coverage and slope conditions through controlled artificial rainfall experiments. Key findings include: (1) biocrusts coverage demonstrated a linear relationship with initial runoff generation time and an exponential relationship with stable runoff generation time. On average, biocrusts delayed initial runoff generation by 396.32 % and extended stable runoff generation time by 153.93 %, thereby increasing the threshold for both initial and stable runoff generation on Pisha-sandstone surfaces. (2) biocrusts reduced runoff volume by an average of 23.89 %, enhanced infiltration volume by 69.19 %, decreased sediment yield by 64.24 %, and lowered the soil erosion modulus by 68.98 %. These results indicated significant promotion of water infiltration and reduction of water erosion. Both effects were positively influenced by coverage and negatively impacted by slope gradient. A critical slope angle of 15 degrees and a critical coverage of 60 % were identified. When the slope was gentle (S 15 degrees), the negative impact of slope predominated, diminishing the positive effect of biocrusts. Additionally, when coverage reached or exceeded 60 %, further increaseing in coverage accelerated the enhancement of infiltration and erosion reduction. Below this threshold, the rate of improvement gradually diminished with increasing coverage. (3) The structural equation model further elucidated that biocrusts mitigate erosion by enhancing the coverage, thereby reducing runoff velocity and modifying the runoff regime. This mechanism effectively dissipates runoff energy, leading to a decreased soil detachment rate and alleviation of soil erosion. Additionally, the relationship between runoff energy and soil detachment rate follows a power function curve, providing an effective method for predicting erosion in Pisha sandstone area. Consequently, biological soil crust technology shows considerable potential for preventing water erosion damage on Pisha sandstone slopes across various gradients.

Background and aims Vascular plants and moss biocrusts are known to coexist in drylands, wherein vascular plant cover is known to be a major influencing factor for biocrusts development. Vascular plants produce litter which may affect moss biocrusts when covering them. However, to which extent the cover of litter may affect the physiology, e.g., photosynthetic activity, of moss biocrusts remains poorly understood.MethodsWe studied the effect of the litter covering on biocrust-forming mosses on the northern Chinese Loess Plateau over four-month period. We used litter from shrubs of Artemisia ordosica and Caragana korshinskii with two levels of litter thickness, and monitored moss greenness, and several indicators of moss physiological activity.ResultsLitter covering reduced moss greenness, content of chlorophyll a and b, soluble sugar, and soluble protein, suggesting a reduced photosynthetic and metabolic activity of mosses under litter cover. On the other hand, mosses covered by litter showed higher contents of malondialdehyde, proline, and catalase activity compared to those mosses without any litter cover, suggesting that litter covering increased oxidative stress in mosses and triggered a protective response against oxidative damage. Moreover, we found litter thickness exerted a more significant impact on the physiological indices of mosses than litter type.ConclusionsOur results demonstrate the detrimental effects of litter covering on the physiological activity of biocrust-forming mosses. The findings provide a mechanistic understanding of the reductions in mosses in ecosystems with high shrub cover, highlighting the importance of litter in mediating the relationships between moss biocrusts and shrub patches.

Biocrust has many ecological roles and the potential for land restoration. Major obstacles to biocrust inoculation in degraded areas are the low physical stability of soil and the frequent wet-dry cycle. Microbially induced carbonate precipitation (MICP) technology, a sand fixation technique, can increase soil stability and decrease soil evaporation. However, it is unclear what the ecological influence of MICP treatment is under the harsh environmental stress. We hypothesized that MICP-treated soil could support biocrust establishment by moderating soil disturbance and improving water retention to mitigate frequent wet-dry cycles. To verify this hypothesis, we prepared cyanobacterial biocrusts (Oscillatoria tenuis) on bare soil and on MICP-treated soil (Sporosarcina pasteurii) and cultivated them for 40 days under high- and low-frequency rainfall. We also simulated disturbance at zero, half, or equal (0, 75, and 150 kJ) the intensity of field conditions during the cultivation. Generalized linear modeling revealed that cyanobacterial biocrust with MICP treatment had high wind erosion resistance but had low indicators of biocrust growth. We also found that MICP treatment facilitated the reduction in chlorophyll content by frequent rainfall and that MICP treatment and physical disturbance had no clear interacting effects on biocrust properties. In summary, our study found MICP treatment could hinder rather than support the cyanobacterial biocrust establishment under the frequent watering and heavy disturbance. Our finding suggests that the appropriate combination of rehabilitation techniques depends on the environmental characteristics of the target area.

Biological soil crusts (BSCs) play a fundamental role in desert ecosystems by stabilizing soil, cycling nutrients, and retaining moisture. However, the assembly processes governing bacterial communities within BSCs remain largely unknown. This study aimed to reveal the spatiotemporal variations in the bacterial community diversity, co-occurrence patterns, and ecological assembly processes of BSCs and their underlying soils across different desert and seasonal conditions. We systematically analyzed the spatial differences in the bacterial diversity, co-occurrence networks, and community assembly processes of BSCs and their underlying soils using samples collected at various soil depths from different BSC types in different deserts. We discovered that BSC type and soil depth were the primary factors driving bacterial community assembly, while seasonal effects were weaker and more indirect, and mainly regulated community dynamics through changes in resource availability and environmental conditions. The underlying soils of moss- and lichen-BSCs exhibited higher bacterial diversity and richness than those of algae BSCs. In contrast, cyano-BSCs exhibited a lower diversity, but Cyanobacteria demonstrated the highest photosynthetic function. Among the different deserts, the community assembly of samples from the eastern Inner Mongolia deserts was largely influenced by environmental selection, whereas stochastic processes were more prominent in the central and western desert regions. A beta-nearest taxon index (beta NTI) analysis indicated that stochastic processes were dominant in surface BSC samples, while environmental selection played a stronger role in deeper layers. A co-occurrence network analysis revealed that surface BSC samples had a high degree of network connectivity, with those from moss- and lichen-BSCs being particularly high, and they also exhibited high modularity and local clustering that promoted the functional stability of the microbial communities. This study revealed the integrated effects of soil depth, BSC type, desert type, and resource availability on microbial community assembly in desert ecosystems. These findings provide a theoretical basis for the microbial management of BSCs and scientific insights to support restoration strategies in desert ecosystems.

Drylands are limited by water and nutrients and exposed to high solar radiation, which result in sparse vegetation cover, soil erosion, and subsequent land degradation. Land degradation affects human wellbeing, causing health and environmental problems, migrations and increasing socio-economic instability worldwide. The restoration of degraded drylands by induced biocrusts has recently gained increased scientific interest. However, harsh environmental conditions can slow down biocrust development. Thus, it is necessary to investigate and develop methods for the mitigation of harsh environmental factors. This survey and assessment reviews studies on environmental barriers to biocrust development and technological achievements in the acceleration of artificially induced biocrust development through the mitigation of harsh environmental conditions. Climatic conditions, and soil and inoculum properties have been identified as major factors that influence the acceleration of biocrust development and which should be considered when dryland restoration is planned. Activities such as watering, shading, soil stabilization and fertilization, as well as further measures for the survival of the cyanobacterial inoculum have promoted biocrust establishment. The restoration of degraded substrates requires the alignment of amelioration techniques with environmental conditions and inoculum requirements. This study has also identified the need for further optimization of watering and shading technologies, better understanding of the importance of soil properties in biocrust growth, as well as further studies on the most appropriate inoculum type and techniques for mass cultivation and application at field scale. The proposal of a multifunctional solution is proposed that could contribute to the restoration of land and cleaner air and water, by providing an inoculum and suitable microsite environmental conditions for the accelerated establishment of viable biocrusts leading to further development, survival, and to the succession to higher organisms under a wide range of environmental conditions.

The study explores the aftermath of a wastewater reservoir failure in a phosphate fertilizer industry, resulting in the release of acidic water containing phosphorus and sulfate compounds into the Ashalim stream's Nature Reserve in the Judean desert, which affected the soil surface biological crusts (biocrusts) layer. The study aims to examine contamination effects on biocrusts over 3 years at two research sites along the stream, compare effects between contaminated sites, assess rehabilitation treatments, and examine their impact on soil characteristics. Hypotheses suggest significant damage to biocrusts due to acidic water flow, requiring human intervention for accelerated restoration. The results indicate adverse effects on biocrust properties, risking its key role in the desert ecosystem. The biocrust layer covering the stream's ground surface suffered significant physical, chemical, and biological damage due to exposure to industrial process effluents. However, soil enrichment treatments, including biocrust components and organic material, show promising effects on biocrust recovery.

The Qinghai-Tibet Plateau is now experiencing ecological degradation risks as a result of climate change and human activities. The alpine grassland ecology in permafrost zones is fragile and susceptible to deterioration due to its high altitude, low temperature, and limited oxygen, which complicates the repair of damaged land. Biological soil crusts (BSCs) are crucial for land restoration in plateau regions because they can thrive in harsh conditions and have environmentally beneficial traits. Inoculated biological soil crust (IBSC) has shown success in low-altitude desert regions, but may not be easily duplicated to the plateau environment. Therefore, it is essential to do a comprehensive and multifaceted analysis of the basic theoretical comprehension and practical application of BSCs on the Tibetan Plateau. This review article aims to provide a brief summary of the ecological significance and the mechanisms related to the creation, growth, and progression of BSCs. It discusses the techniques used for cultivating BSCs in laboratories and using them in the field, focusing on the Qinghai-Tibet Plateau circumstance. We thoroughly discussed the potential and the required paths for further studies. This study may be used as a basis for selecting suitable microbial strains and accompanying supplemental actions for implementing IBSCs in the Qinghai-Tibet Plateau.

Spring, especially the freeze-thaw season, is considered the key period for the growth and carbon sequestration of desert mosses. It is not clear how the change in environment water and temperature affects the physiological characteristics of desert mosses in freeze-thaw season. In this study, the effects of water and freeze-thaw cycles on the physiological characteristics of Syntrichia caninervis were assessed by manipulating the increase or removal of 65% snow and changes in the freeze-thaw cycles. The results showed that the changes in snow depth, freeze-thaw cycles, and their interaction significantly affected the plant water content, osmoregulatory substances content, antioxidant substance, and antioxidant enzyme activities. The contents of free proline, soluble sugar, ascorbic acid (AsA), reduced glutathione (GSH), and malondialdehyde (MDA), and superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities increased significantly with the decrease in snow depth and freeze-thaw cycles. POD and free proline were the most sensitive to the snow depth and freeze-thaw cycles, while SOD and CAT were the least sensitive. Therefore, compared with the increase in freeze-thaw cycles, the reduction in freeze-thaw cycles weakened the physiological sensitivity of S. caninervis to snow depth changes.