Deinococcus species, noted for their exceptional resistance to DNA-damaging environmental stresses, have piqued scientists' interest for decades. This study dives into the complex mechanisms underpinning radiation resistance in the Deinococcus genus. We have examined the genomes of 82 Deinococcus species and classified radiation-resistance proteins manually into five unique curated categories: DNA repair, oxidative stress defense, Ddr and Ppr proteins, regulatory proteins, and miscellaneous resistance components. This classification reveals important information about the various molecular mechanisms used by these extremophiles which have been less explored so far. We also investigated the presence or lack of these proteins in the context of phylogenetic relationships, core, and pan-genomes, which offered light on the evolutionary dynamics of radiation resistance. This comprehensive study provides a deeper understanding of the genetic underpinnings of radiation resistance in the Deinococcus genus, with potential implications for understanding similar mechanisms in other organisms using an interactomics approach. Finally, this study reveals the complexities of radiation resistance mechanisms, providing a comprehensive understanding of the genetic components that allow Deinococcus species to flourish under harsh environments. The findings add to our understanding of the larger spectrum of stress adaption techniques in bacteria and may have applications in sectors ranging from biotechnology to environmental research.

为研究埋地二氧化碳管道周围土壤温度场的分布规律，基于齐鲁石化-胜利油田百万吨级CCUS示范项目二氧化碳输送管道，建立管道周围土壤温度场数值模型，采用Fluent软件对二氧化碳三种输送相态（低压液相、高压液相、超临界）下的典型工况土壤温度场进行计算。在低压液相输送的基础上分析管道周围土壤冻结范围的季节变化规律及不同埋深、不同运行温度对管道周围土壤冻结范围的影响，研究结果表明：随着埋深增大，管道周围多年冻土范围增大，季节性冻土范围受影响较小；而随着起点温度的升高，管道周围多年冻土和季节性冻土范围都减小，当温度升高至0℃时将不再形成冻土。在高压液相输送和超临界输送的基础上，以小麦为例分析管道运行温度变化对地表植被的影响，研究结果表明：为使土壤温度能满足沿线农作物生长需求，二氧化碳高压液相输送时，其管输介质温度不宜低于5℃，超临界输送时不宜高于50℃。

为研究埋地二氧化碳管道周围土壤温度场的分布规律，基于齐鲁石化-胜利油田百万吨级CCUS示范项目二氧化碳输送管道，建立管道周围土壤温度场数值模型，采用Fluent软件对二氧化碳三种输送相态（低压液相、高压液相、超临界）下的典型工况土壤温度场进行计算。在低压液相输送的基础上分析管道周围土壤冻结范围的季节变化规律及不同埋深、不同运行温度对管道周围土壤冻结范围的影响，研究结果表明：随着埋深增大，管道周围多年冻土范围增大，季节性冻土范围受影响较小；而随着起点温度的升高，管道周围多年冻土和季节性冻土范围都减小，当温度升高至0℃时将不再形成冻土。在高压液相输送和超临界输送的基础上，以小麦为例分析管道运行温度变化对地表植被的影响，研究结果表明：为使土壤温度能满足沿线农作物生长需求，二氧化碳高压液相输送时，其管输介质温度不宜低于5℃，超临界输送时不宜高于50℃。

Strain Y74(T)was an isolate from the sandy soil in the town of Huatugou, Qinghai-Tibet Plateau, China. An analysis of this strain's phenotypic, chemotaxonomic, and genomic characteristics established the relationship of the isolate with the genusPlanococcus. Strain Y74(T)was able to grow between 4 and 42 degrees C (with an optimum temperature of 28 degrees C) at pH values of 6-8.5 and in 0%-7% (w/v) NaCl. The dominant quinones were MK-8 and MK-7. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, and an unknown phospholipid. The majority of the fatty acid content was anteiso-C-15:0(28.8%) followed by C-16:1 omega 7c alcohol (20.9%) and iso-C-14:0(13.4%). The 16S rRNA gene sequence similarity analysis demonstrated a stable branch formed by strain Y74(T)andPlanococcus halotoleransSCU63(T)(99.66%). The digital DNA-DNA hybridization between these two strains was 57.2%. The G + C content in the DNA of Y74(T)was 44.5 mol%. In addition, the morphological, physiological, and chemotaxonomic pattern clearly differentiated the isolates from their known relatives. In conclusion, the strain Y74(T)(=JCM 32826(T) = CICC24461(T)) represents a novel member of the genusPlanococcus,for which the namePlanococcus antioxidanssp. nov. is proposed. Strain Y74(T)was found to have potent antioxidant activity via its hydrogen peroxide tolerance and its 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity. The DPPH radical-scavenging activity was determined to be 40.2 +/- 0.7%. The genomic analysis indicated that six peroxidases genes, one superoxide dismutase gene, and one dprA (DNA-protecting protein) are present in the genome of Y74(T).