Abandoned farmlands are increasing due to socio-economic changes and land marginalization, and they require sustainable land management practices. Biocrusts are a common cover on the topsoil of abandoned farmlands and play an important role in improving soil stability and erosion resistance. The critical functions of biocrusts are known to mostly rely on their biofilaments and extracellular polymeric substances (EPS), but how these components act at microscopic scale is still unknown, while rheological methods are able to provide new insights into biocrust microstructural stability at particle scale. Here, bare soil and two representative types of biocrusts (cyanobacterial and moss crusts) developed on sandy (Ustipsamments) and sandy loam (Haplustepts) soils in abandoned farmlands in the northern Chinese Loess Plateau were collected at a sampling depth of 2 cm. Changes in the rheological properties of the biocrusts were analyzed with respect to their biofilament network and EPS contents to provide possible explanations. The rheological results showed that compared with bare soil, storage and loss moduli were decreased by the biocrusts on sandy soil, but they were increased by the biocrusts on sandy loam soil. Other rheological parameters tau max, gamma L, gamma YP, and Iz of biocrusts on both soils were significantly higher than those of bare soil, showing higher viscoelasticity. And the moss crusts had about 10 times higher rheological property values than the cyanobacterial crusts. Analysis from SEM images showed that the moss crusts had higher biofilament network parameters than the cyanobacterial crusts, including nodes, crosslink density, branches, branching ratio and mesh index, and biofilament density, indicating that the biofilament network structure in the moss crusts was more compact and complex in contrast to the cyanobacterial crusts. Additionally, EPS content of the moss crusts was higher than that of the cyanobacterial crusts on both soils. Overall, the crosslink density, biofilament density, and EPS content of the biocrusts were significantly and positively correlated with their gamma YP and Iz. The interaction between crosslink density and biofilament density contributed 73.2 % of gamma YP, and that between crosslink density and EPS content contributed 84.0 % of Iz. Our findings highlight the biocrusts-induced changes of abandoned farmland soil rheological properties in drylands, and the importance of biocrust biofilament network and EPS in maintaining abandoned farmland soil microstructural stability to resist soil water/wind erosion and degradation, providing a new perspective for sustainable management of abandoned farmlands.

In performance-based design, it is crucial to understand deformation characteristics of geocell layers in soil under footing loads. To explore this, a series of laboratory loading tests were carried out to investigate the influence of varying parameters on the strain levels within the geocell layer in a sandy soil under axial strip footing loading. The results were analyzed in terms of maximum strain levels, strain variation along the geocell layer and the correlation between horizontal and vertical strains. In this study, the maximum observed strain levels for geocellreinforced strip footing systems reached 2.3 % for horizontal (tensile) strain and 1.4 % for vertical (compressive) strain. Furthermore, most strain levels were concentrated within a distance of 1.5 times the footing width from the axis of strip footing. In geocell-reinforced footing systems, the interaction between horizontal and vertical strains becomes a key factor, with the ratio of horizontal to vertical cell wall strains ranging approximately from 1 to 2.5. The outcomes of this study are expected to contribute to the practical applications of geocell-reinforced footing systems.

Intervertebral disc degeneration (IVDD) is a globally prevalent disease, yet achieving dual repair of tissue and function presents significant challenges. Considering reactive oxygen species (ROS) is a primary cause of IVDD, and given the decrease of nucleus pulposus cells (NPCs) and extensive degradation of extracellular matrix (ECM) during IVDD development, the present study, inspired by the seeds-and-soil strategy, has developed NPCsloaded TBA@Gel&Chs hydrogel microspheres. These microspheres serve as exogenous supplements of NPCs and ECM analogs, replenishing seeds and soil for nucleus pulposus repair, and incorporating polyphenol antioxidant components to interrupt the oxidative stress-IVDD cycle, thereby constructing a microsphere system where NPCs and ECM support each other. Experiments proved that TBA@Gel&Chs exhibited significant extra-cellular ROS-scavenging antioxidant capabilities while effectively upregulating intracellular antioxidant proteins expression (Sirt3 and Sod2). This dual-action antioxidant capability effectively protects the vitality and physiological functions of NPCs. The therapeutic effects of microspheres on IVDD were also confirmed in rat models, which was found significantly restore histological structure and mechanical properties of degenerated discs. Additionally, RNA-seq results have provided evidences of antioxidant mechanism by which TBA@Gel&Chs protected NPCs from oxidative stress. Therefore, the NPCs-loaded TBA@Gel&Chs microspheres developed in this study have achieved excellent therapeutic effects, offering a paradigm using antioxidant biomaterials combined with cellular therapy for IVDD treatment.

Zn2+ play an important role in maintaining the normal functioning of living organisms, and excessive or insufficient levels can cause serious health problems. Zn2+ play a vital role in maintaining normal biological functions, and abnormal levels Zn2+ may lead to a range of severe health issues. Therefore, real-time and accurate detection of Zn2+ is critically important. Given the widespread presence of Zn2+ in living organisms and external environments, developing probes suitable for multi-scenario Zn2+ detection is of significant practical value. In this study, a novel probe SSD was synthesized using salicylaldehyde as the precursor, enabling ultra-sensitive Zn2+ detection with a detection limit as low as 9.1 nM. The probe SSD was successfully applied to the detection of Zn2+ in water, soil, and food samples. In addition, an SSD-based Zn2+ smartphone detection platform was developed, which can quickly detect the content of Zn2+ in actual samples. Moreover, due to its excellent optical properties and low toxicity, SSD was able to detect both intracellular and extracellular Zn2+. Most importantly, probe SSD demonstrated the capability to monitor real-time changes in Zn2+ concentrations during cellular oxidative damage, providing valuable insights for research on related physiological diseases.

Buried pipes are subjected to static and dynamic loads depending on their areas of use. To mitigate the risk of damage caused by these effects, various materials and reinforcement methods are utilized. In this study, five buried uPVC pipes designed in accordance with ASTM D2321 standards were reinforced with three different ground improvement materials: Geocell, Geonet, and Geocomposite, and experimentally subjected to dynamic impact loading. Acceleration, velocity, and displacement values were obtained from the experiments. Subsequently, finite element analysis (FEA) was performed using the ABAQUS software to determine stress values and volumetric displacements in the pipes, and the model was validated with a 5-7% error margin. In the final stage of the study, a parametric analysis was conducted by modifying the soil cover height above the pipe and the Geocell thickness in the validated finite element model. The parametric study revealed that the displacement value in the pipe decreased by 78% with an increase in soil cover height, while a 16% reduction was observed with an increase in Geocell thickness. The results demonstrate that the soil improvement techniques examined in this study provide an effective solution for enhancing the impact resistance of buried pipeline systems.

Subsea pipelines in Arctic environments face the risk of damage from ice gouging, where drifting ice keels scour the seabed. To ensure pipeline integrity, burial using methods like ploughs, mechanical trenchers, jetting, or hydraulic dredging is the conventional protection method. Each method has capabilities and limitations, resulting in different trench profiles and backfill characteristics. This study investigates the influence of these trenching methods and their associated trench geometries on pipeline response and seabed failure mechanisms during ice gouging events. Using advanced large deformation finite element (LDFE) analyses with a Coupled Eulerian-Lagrangian (CEL) algorithm, the complex soil behavior, including strain-rate dependency and strainsoftening effects, is modeled. The simulations explicitly incorporate the pipeline, enabling a detailed analysis of its behavior under ice gouging loads. The simulations analyze subgouge soil displacement, pipeline displacement, strains, and ovalization. The findings reveal a direct correlation between increasing trench wall angle and width and the intensification of the backfill removal mechanism. Trench geometry significantly influences the pipeline's horizontal and vertical displacement, while axial displacement and ovalization are less affected. This study emphasizes the crucial role of trenching technique selection and trench shape design in mitigating the risks of ice gouging, highlighting the value of numerical modeling in optimizing pipeline protection strategies in these challenging environments.

In sensitive ecosystems of the Arctic, even slight disruptions may produce serious damage. Therefore, the extent of contamination in such zones should be evaluated. A comparison was made between concentrations of metals in Sanionia uncinata in three areas of the European Arctic: (1) the vicinity of the Polish Polar Station in the SW part of Spitsbergen on Wedel Jarlsberg Land, (2) Longyearbyen (Spitsbergen) influenced by local sources of pollution and (3) Iceland relatively free from local pollution. The tested hypothesis was that S. uncinata from Iceland contains significantly lower concentrations of metals than the same moss from Spitsbergen. The maximum concentrations of metals in the examined moss from Longyearbyen reached values for Cr and Mn higher than those known as harmful for plants and for Ni and Zn values within the harmful ranges with no visible harmful effects. S. uncinata from Iceland contained significantly lower concentrations of Cd, Mn, Pb compared to this species from Spitsbergen. S. uncinata seems to be a useful indicator for metal fallout in the European Arctic. This study presents the effects of local sources of contamination on metal levels in S. uncinata from Longyearbyen, Wedel Jarlsberg Land and Iceland as well as verification of S. uncinata as a suitable bioindicator in this Arctic area. The benefit of the study is a to better understanding contamination problems of Arctic habitats.

Atrazine (ATR) is a widely utilized herbicide that has been demonstrated to exert a multitude of deleterious effects on the environment, particularly with regard to water and soil contamination. Moreover, its disruption of endocrine function and implications for antibiotic resistance underscore the urgent need to prioritize alternative solutions for both ecosystems and human health. Therefore, the objective of this study was to investigate a range of neurotoxic effects associated with atrazine-induced damage in the prefrontal lobe of mice. The results of this study indicate that treatment with ATR in C57BL/6 J mice resulted in cognitive-related behavioral deficits, including anxiety and depression, as well as motor impairments. In vivo analyses demonstrated that ATR exposure resulted in a reduction in neuronal synapse density at the microstructural level, while also compromising prefrontal morphological integrity, nociceptor count, and overall neuronal health within the brain. These findings collectively suggest that synaptic deficits are implicated in ATR-induced behavioral abnormalities observed in these mice. Furthermore, our findings revealed that ATR exposure resulted in elevated TDP-43 expression levels that were ectopically localized within the cytoplasm. This alteration led to impaired functionality of mRNP granules and contributed to the development of abnormal synaptic defects. Conversely, TDP43 has the potential to localize ectopically to mitochondria, where it activates the mitochondrial unfolded protein response (UPRmt), which ultimately results in mitochondrial dysfunction. These findings collectively indicate a strong correlation between TDP-43 dysregulation and the progression of neurodegenerative diseases. Further investigation into the potential neurotoxicity of atrazine may foster heightened awareness, leading to more stringent regulatory measures, research into safer alternatives, and the adoption of sustainable practices, which are essential for safeguarding environmental integrity alongside human health.

Geocells are three-dimensional, interconnected cellular geosynthetics widely used to enhance the overall strength of soils. Their foldable structure can cause variations in pocket shape during installation, depending on the extent of extension. Understanding the impact of these shape variations is essential for optimizing reinforcement efficiency and reducing the associated geocell application costs. The aspect ratio, defined as the ratio of the cell's transverse (welded) axis to the longitudinal (wall summit) axis, is proposed to evaluate the degree of extension of the most commonly utilized honeycomb-shaped geocell. A coupled continuum-discontinuum numerical method was employed to investigate the behavior of honeycomb-shaped geocell reinforced soils across various aspect ratios under confined compressive loading. The simulation results indicate that a geocell with an aspect ratio of 1.0 exhibits optimal reinforcement efficiency, and whereas reinforcement efficiency decreases as the aspect ratio deviates from 1.0 causing pocket geometries to flatten. The superior performance of rounded geocells is attributed to their enhanced ability to promote load-bearing in strong contact subnetworks. This results in denser packing structures, higher contact force anisotropy from a microscopic perspective, and greater confinement capacity against deformation from a macroscopic perspective.

Rare earth elements (REEs) are a type of frequently reported emerging pollutant that affects plant growth. The harm caused by continuous exposure to low-dose REEs has rarely been studied. Quickly, accurately, and noninvasively monitoring the continuous influence of low-dose REEs on plant growth in situ is key to indicating and warning of its harm to plants and ecosystems. In this study, after continuous exposure to low-dose lanthanum [La(III), a REE] for 14 days, invisible damage occurred in leaf cells, and La accumulated continuously in the soybean plants (leaves > stems > roots > pods > seeds), causing potential human health risks. Two proteins [vitronectin-like protein (VN) and arabinogalactan proteins (AGP)] in leaf cells that bound La(III) were selected as biomarkers, and changes in these two proteins were detected by constructing dual-sensors in living leaf cells after continuous exposure to low-dose La(III) for 14 days. The results showed that the electrochemical outputs from leaf cells-the electron transfer resistance Ret(VN) and Ret(AGP)-were related to the damage indices such as MDA, chlorophyll content, electrolyte leakage, cell vitality, fresh and dry weight of leaves, and leaf area. Using this output, two warning intervals of visible damage were obtained: Ret(VN) was 8.53 %-47.22 %, and Ret(AGP) was 12.75 %-51.31 %. This study successfully demonstrated the real-time in situ detection of plant cell biomarker changes and invisible damage under low-dose La(III) exposure, providing methods for early warning monitoring of plant damage caused by low-dose continuous exposure to REEs.