Drought stress negatively affects cotton pollen fertility, which in turn leads to a decrease in seed number per boll and boll weight. Exogenous melatonin application significantly enhances pollen fertility under drought conditions, while the specific underlying mechanisms remain unclear. A pot experiment was conducted using a cultivar Yuzaomian 9110 under two moisture treatments (soil relative water content at 75 +/- 5 % and 45 +/- 5 %) with two melatonin concentration (0 and 200 mu M) to investigate the effects of exogenous melatonin on the structural traits and physiological metabolism of cotton anthers and its' relationships with pollen fertility. Results demonstrated the significant impact of drought on anthers development and metabolism, with damage to the anther tapetum and decreased starch and adenosine triphosphate (ATP) contents, subsequently resulting in reduced pollen germination rate, seed number per boll and boll weight. Melatonin application in water-deficit anthers up-regulated the expression of sucrose transporter protein (GhSWEET55) and phosphate sucrose synthetase, promoting sucrose import and synthesis, respectively. However, it also increased sucrose synthase and acid convertase, accelerating sucrose decomposition and reducing its content. Additionally, melatonin application promoted starch accumulation in water-deficit anthers by enhancing activities of adenosine diphosphate glucose pyrophosphorylase and soluble starch synthase, meaning that potential energy storage was increased, which facilitated the formation of pollen fertility. Although melatonin application reduced the expression of pyruvate kinase (GhPK) and glucose 6-phosphogluconate dehydrogenase (GhG6PD) genes in water-deficit anthers, it upregulated hexokinase (GhHXK) and citrate synthase (GhCIT) expression, enhancing ATP content, and ultimately pollen fertility, seed number and boll weight under drought. In summary, exogenous melatonin preserved cotton pollen fertility under drought stress by regulating carbohydrate and energy metabolism, especially enhancing starch and ATP accumulation in anthers.

The root, a key organ for sensing the soil environment, is easily damaged by environmental stresses such as low soil temperature. Although the exact mechanism is unknown, exogenous sucrose can mitigate the oxidative damage to the root caused by low temperatures in the root zone. In this study, we examined how exogenous sucrose affected the transcriptome and physiology of Malus baccata Borkh. seedling roots at sub-low root-zone temperature (LRT). The exogenous sucrose treatment was more effective than other treatments in mitigating LRT stress injury. This was achieved by decreasing reactive oxygen species (O 2 .- and H2O2) 2 O 2 ) and malondialdehyde content, increasing the activities of antioxidant enzymes (SOD, POD, CAT, APX, GR, and MDHAR), increasing AsA and GSH content, and increasing soluble sugar content. Transcriptome analysis revealed that alpha-linolenic acid metabolism, fatty acid biosynthesis, phenylpropane biosynthesis, and glycolysis/gluconeogenesis were the primary areas of enrichment for the differentially expressed genes identified under the LRT treatment. Exogenous sucrose may enhance the tolerance of Malus baccata Borkh. to LRT by regulating the expression of differentially expressed genes ( GST, LOX, SS, PFK, ADH, , and 4CL) ) related to the antioxidant system, carbohydrate metabolism, alpha-linolenic acid metabolism, and phenylpropane biosynthesis pathways. These results offer a foundation for additional investigation into the molecular mechanism underlying the modulation of the root response to low temperature by exogenous sucrose.

Soil salinity poses a significant threat to agricultural productivity, impacting the growth and yield of wheat (Triticum aestivum L.) plants. This study investigates the potential of melatonin (MT; 100 mu M) and hydrogen sulfide (H2S; 200 mu M sodium hydrosulfide, NaHS) to confer the tolerance of wheat plants to 100 mM NaCl. Salinity stress induced the outburst of reactive oxygen species (ROS) resulting in damage to the chloroplast structure, growth, photosynthesis, and yield. Application of either MT or NaHS augmented the activity of antioxidant enzymes, superoxide dismutase, ascorbate peroxidase, glutathione reductase, and reduced glutathione (GSH) levels, upregulated the expression of Na+ transport genes (SOS1, SOS2, SOS3, NHX1), resulting in mitigation of salinity stress. Thus, improved stomatal behavior, gas-exchange parameters, and maintenance of chloroplast structure resulted in enhanced activity of the Calvin cycle enzymes and overall enhancement of growth, photosynthetic, and yield performance of plants under salinity stress. The use of DL-propargylglycine (PAG, an inhibitor of hydrogen sulfide biosynthesis) and p-chlorophenyl alanine (p-CPA, an inhibitor of melatonin biosynthesis) to plants under salt stress showed the comparative necessity of MT and H2S in mitigation of salinity stress. In the presence of PAG, more pronounced detrimental effects were observed than in the presence of p-CPA, emphasizing that MT was involved in mitigating salinity through various potential pathways, one of which was through H2S.