Soil freeze-thaw cycles (FTCs) are common in temperate agricultural ecosystems during the non-growing season and are progressively influenced by climate change. The impact of these cycles on soil microbial communities, crucial for ecosystem functioning, varies under different agricultural management practices. Here, we investigated the dynamic changes in soil microbial communities in a Mollisol during seasonal FTCs and examined the effects of stover mulching and nitrogen fertilization. We revealed distinct responses between bacterial and fungal communities. The dominant bacterial phyla reacted differently to FTCs: for example, Proteobacteria responded opportunistically, Actinobacteria, Acidobacteria, Choroflexi and Gemmatimonadetes responded sensitively, and Saccharibacteria exhibited a tolerance response. In contrast, the fungal community composition remained relatively stable during FTCs, except for a decline in Glomeromycota. Certain bacterial OTUs acted as sensitive indicators of FTCs, forming keystone modules in the network that are closely linked to soil carbon, nitrogen content and potential functions. Additionally, neither stover mulching nor nitrogen fertilization significantly influenced microbial richness, diversity and potential functions. However, over time, more indicator species specific to these agricultural practices began to emerge within the networks and gradually occupied the central positions. Furthermore, our findings suggest that farming practices, by introducing keystone microbes and changing interspecies interactions (even without changing microbial richness and diversity), can enhance microbial community stability against FTC disturbances. Specifically, higher nitrogen input with stover removal promotes fungal stability during soil freezing, while lower nitrogen levels increase bacterial stability during soil thawing. Considering the fungal tolerance to FTCs, we recommend reducing nitrogen input for manipulating bacterial interactions, thereby enhancing overall microbial resilience to seasonal FTCs. In summary, our research reveals that microbial responses to seasonal FTCs are reshaped through land management to support ecosystem functions under environmental stress amid climate change.

Among the most widespread structures for successfully retaining water and checking erosion on the semi-arid portions of China's Loess Plateau, check dams retain silt at slower than projected rates, leading to flood control issues. Meanwhile, the shortage and the uneven distribution of time and space of water resources in semi-arid areas can easily cause droughts and floods, which seriously restricted the rapid development of the socioeconomic. However, some of the high-quality rain and flood resources accumulated in the check dams can be used to alleviate part of the water resources crisis instead of causing flood. With the goal of holistically maximizing a projected check dam array's water resource, ecosystem and socioeconomic benefits, a Check Dam Benefit Maximization Model (CDBMM) was first developed. The CDBMM was first applied to the Si Jiagou Basin, and the model showed the total costs represent 7.07% of the total and rather significant benefits. Water resources benefits accounting for 45.40% of the total benefits, indicating that the water resources benefits were substantial and should be considered as the main influencing factors in the basin's ecosystem-friendly design and construction. Use of the CDBMM in watershed planning will allow a more efficient use of water and soil resources and greatly alleviate water crises in the semi-arid area. It can further provide a reference for both check dam system planning and the system benefits analysis.

BackgroundAntarctica and its unique biodiversity are increasingly at risk from the effects of global climate change and other human influences. A significant recent element underpinning strategies for Antarctic conservation has been the development of a system of Antarctic Conservation Biogeographic Regions (ACBRs). The datasets supporting this classification are, however, dominated by eukaryotic taxa, with contributions from the bacterial domain restricted to Actinomycetota and Cyanobacteriota. Nevertheless, the ice-free areas of the Antarctic continent and the sub-Antarctic islands are dominated in terms of diversity by bacteria. Our study aims to generate a comprehensive phylogenetic dataset of Antarctic bacteria with wide geographical coverage on the continent and sub-Antarctic islands, to investigate whether bacterial diversity and distribution is reflected in the current ACBRs.ResultsSoil bacterial diversity and community composition did not fully conform with the ACBR classification. Although 19% of the variability was explained by this classification, the largest differences in bacterial community composition were between the broader continental and maritime Antarctic regions, where a degree of structural overlapping within continental and maritime bacterial communities was apparent, not fully reflecting the division into separate ACBRs. Strong divergence in soil bacterial community composition was also apparent between the Antarctic/sub-Antarctic islands and the Antarctic mainland. Bacterial communities were partially shaped by bioclimatic conditions, with 28% of dominant genera showing habitat preferences connected to at least one of the bioclimatic variables included in our analyses. These genera were also reported as indicator taxa for the ACBRs.ConclusionsOverall, our data indicate that the current ACBR subdivision of the Antarctic continent does not fully reflect bacterial distribution and diversity in Antarctica. We observed considerable overlap in the structure of soil bacterial communities within the maritime Antarctic region and within the continental Antarctic region. Our results also suggest that bacterial communities might be impacted by regional climatic and other environmental changes. The dataset developed in this study provides a comprehensive baseline that will provide a valuable tool for biodiversity conservation efforts on the continent. Further studies are clearly required, and we emphasize the need for more extensive campaigns to systematically sample and characterize Antarctic and sub-Antarctic soil microbial communities.APsmQ8MphSAgg4BzZyqdNTVideo AbstractConclusionsOverall, our data indicate that the current ACBR subdivision of the Antarctic continent does not fully reflect bacterial distribution and diversity in Antarctica. We observed considerable overlap in the structure of soil bacterial communities within the maritime Antarctic region and within the continental Antarctic region. Our results also suggest that bacterial communities might be impacted by regional climatic and other environmental changes. The dataset developed in this study provides a comprehensive baseline that will provide a valuable tool for biodiversity conservation efforts on the continent. Further studies are clearly required, and we emphasize the need for more extensive campaigns to systematically sample and characterize Antarctic and sub-Antarctic soil microbial communities.APsmQ8MphSAgg4BzZyqdNTVideo Abstract

Soil supports life by serving as a living, breathing fabric that connects the atmosphere to the Earth's crust. The study of soil science and pedology, or the study of soil in the natural environment, spans scales, disciplines, and societies worldwide. Soil science continues to grow and evolve as a field given advancements in analytical tools, capabilities, and a growing emphasis on integrating research across disciplines. A pressing need exists to more strongly incorporate the study of soil, and soil scientists, into research networks, initiatives, and collaborations. This review presents three research areas focused on questions of central interest to scientists, students, and government agencies alike: 1) How do the properties of soil influence the selection of habitat and survival by organisms, especially threatened and endangered species struggling in the face of climate change and habitat loss during the Anthropocene? 2) How do we disentangle the heterogeneity of abiotic and biotic processes that transform minerals and release life-supporting nutrients to soil, especially at the nano-to microscale where mineral-water-microbe interactions occur? and 3) How can soil science advance the search for life and habitable environments on Mars and beyond-from distinguishing biosignatures to better utilizing terrestrial analogs on Earth for planetary exploration? This review also highlights the tools, resources, and expertise that soil scientists bring to interdisciplinary teams focused on questions centered belowground, whether the research areas involve conservation organizations, industry, the classroom, or government agencies working to resolve global chal-lenges and sustain a future for all.

Under conditions of ongoing climate warming and increasing anthropogenic impact on land resources, the use of moisture-saving soil-protecting technologies becomes especially important. Snow cover is of great importance for agriculture, as it changes radiation and thermal balance of underlying surface, protecting soil from cooling and winter crops from freezing, accumulates winter precipitation, being the most important source of increasing soil moisture reserves in arid and subarid zones in spring. Winter precipitation accounts for up to 30% of the annual norm. Soil moisture reserves formed with their help take up to 42% in total water consumption for grain crop yield formation during wet summer and up to 75% during dry summer. This article aims to study the effect of different methods of snow retention and snow cover height on the yield of grain crops. An effective method of snow retention is leaving high stubble after harvesting winter and spring crops. Leaving stubble bushes with a height of 0.35-0.40 m and a width of 1.5 m every 4.5 m provides accumulation of a solid snow cover in steppe areas with a height of 0.30-0.35 m, which increases the yield of wheat. Waders provide a more uniform distribution of snow cover than forest strips. Climate change contributes to the fact that snow retention becomes an urgent problem not only in the dry steppe, but also to the north - even in the forest-steppe. Creation of snow retarders was done on Vetelny state farm, located in Balashovsky district in the western part of Saratov region, in the steppe zone, where chernozem soils prevail in the soil cover. In the autumn period, snow barriers were installed, and in the winter period, their effect on snow accumulation was studied. The study of the effect of snow barriers on soil moisture accumulation during the growing season of winter wheat was compared in the zones of dry steppe, steppe and forest-steppe. It was revealed that during regrowth of winter wheat the least amount of productive moisture stocks in 0-1.0 m soil layer was contained in dry steppe 1377 m(3).ha(-1), the highest in forest-steppe zone up to 1841 m(3).ha(-1). Snow retention increased the amount of moisture in the soil in the dry steppe, steppe and foreststeppe zone by 251, 151, 115-131 m(3).ha(-1), or 18, 10, 6-8%, respectively. Thus, rational use of winter precipitation is a significant reserve of agricultural landscape productivity increase, especially in dry-steppe areas.

Part 1 of this review synthesizes recent research on status and climate vulnerability of freshwater and saltwater wetlands, and their contribution to addressing climate change (carbon cycle, adaptation, resilience). Peatlands and vegetated coastal wetlands are among the most carbon rich sinks on the planet sequestering approximately as much carbon as do global forest ecosystems. Estimates of the consequences of rising temperature on current wetland carbon storage and future carbon sequestration potential are summarized. We also demonstrate the need to prevent drying of wetlands and thawing of permafrost by disturbances and rising temperatures to protect wetland carbon stores and climate adaptation/resiliency ecosystem services. Preventing further wetland loss is found to be important in limiting future emissions to meet climate goals, but is seldom considered. In Part 2, the paper explores the policy and management realm from international to national, subnational and local levels to identify strategies and policies reflecting an integrated understanding of both wetland and climate change science. Specific recommendations are made to capture synergies between wetlands and carbon cycle management, adaptation and resiliency to further enable researchers, policy makers and practitioners to protect wetland carbon and climate adaptation/resiliency ecosystem services.