Integrating cover crops and bionematicides presents a sustainable approach to managing plant-parasitic nematodes (PPN) in organic vegetable production systems. The integration of sunn hemp, Crotalaria juncea ('Crescent sun') and sorghum-sudangrass, Sorghum bicolor x S. sudanense ('Sweet Six BMR') with bionematicides was evaluated in two locations in central (Gulf Coast Research and Educational Centre-GCREC) and south (Fort Lauderdale Research and Educational Centre-FLREC) Florida for the effectiveness of PPN suppression. Field experiments were conducted with establishing cover crops in each location 3 months before planting organic zucchini on plastic beds equipped with a drip application system used to inject three commercial bionematicides (thyme oil, neem oil and azadirachtin) and the broth culture of Xenorhabdus bovienii bacteria associated with Steinernema feltiae. Cover cropping with sunn hemp and sorghum-sudangrass significantly reduced population densities of root-knot nematodes (Meloidogyne spp.) at GCREC, whereas only sunn hemp reduced the root-knot nematode population at FLREC. Galling severity on zucchini roots caused by Meloidogyne spp. was significantly lower in azadirachtin and neem oil applications integrated with sunn hemp. The impact of integrating cover crops with bionematicides on other PPN, such as Mesocriconema spp., Nanidorus minor and Hoplolaimus spp., varied among the treatments at both locations. Integrating cover crops with bionematicide applications provided additional control options for zucchini, but the efficacy of different bionematicides depended on the nematode species present in the soil and the cover crop species used. These findings underscore the importance of adaptive nematode management, where control strategies are customised to target the specific nematode populations causing economic damage in each field.

Herbicides are important for weed control but can severely impact ecosystems, causing soil and water contamination, biodiversity loss, and harm to non-target organisms. Tebuthiuron, widely used in sugarcane cultivation, is highly soluble and persistent, posing significant environmental risks. Microbial inoculation has emerged as a sustainable strategy to mitigate such damage. This study investigated the phytoremediation potential of Mucuna pruriens and Canavalia ensiformis in tebuthiuron-contaminated soils, enhanced by fungal and bacterial inoculants. Crotalaria juncea served as a bioindicator plant, and Lactuca sativa was used in ecotoxicological bioassays. During a 140-day greenhouse experiment from September 2021 to March 2022, M. pruriens showed faster growth than C. ensiformis in uncontaminated soils but was more affected by tebuthiuron. Bacterial inoculants improved M. pruriens growth under stress, while fungal inoculants mitigated tebuthiuron's effects on C. ensiformis. C. juncea exhibited high sensitivity to tebuthiuron but grew beyond 100 cm with bacterial inoculants. Ecotoxicological assays showed that bacterial bioaugmentation significantly reduced soil toxicity. Natural attenuation further decreased tebuthiuron toxicity, and prior cultivation of M. pruriens enhanced soil detoxification. This integrated approach combining phytoremediation and bioaugmentation offers a sustainable method to degrade tebuthiuron, foster safer agriculture, and reduce environmental and health risks.