Four undescribed cytochalasins (1-4), three undescribed orsellinic acid derivatives (5-7) and two known metabolites including methyl lecanorate (8) and methyl orsellinate (9) were isolated from the solid-state cultivation of a soil-derived fungus Trichocladium asperum SQ2-3 collected in Qinghai-Tibet Plateau. Their structures were elucidated by analysis of NMR (1D and 2D) and mass spectrometry data. The absolute configurations of 1- 7 were assigned by a combination of the modified Mosher's method, microscale derivatization and Mo2(OAc)4-induced circular dichroism experiment. Compounds 1 , 2 , 3 and 6 showed significant cytotoxicity against HL-60, A3494, SMMC-7721, MDA-MB-231 and SW480 cell lines with IC50 values ranging from 4.74 to 15.84 mu M, respectively. Meanwhile, compound 1 could obviously damage mitochondrial membrane potential and induce G2/M cell cycle arrest in A549 cells.

This study explored morphological, physiological, molecular, and epigenetic responses of tomatoes (Solanum lycopersicum) to soil contamination with polyethylene nanoplastics (PENP; 0.01, 0.1, and 1 gkg-1 soil). The PENP pollution led to severe changes in plant morphogenesis. The PENP treatments were associated with decreased plant biomass, reduced internode length, delayed flowering, and prolonged fruit ripening. Abnormal inflorescences, flowers, and fruits observed in the PENP-exposed seedlings support genetic changes and meristem dysfunction. Exposure of seedlings to PENP increased H2O2 accumulation and damaged membranes, implying oxidative stress. The PENP treatments induced activities of catalase (EC1.11.1.6), peroxidase (EC1.11.1.7), and phenylalanine ammonia-lyase (EC4.3.1.24) enzymes. Soil contamination with PENP also decreased the net photosynthesis, maximum photosystem efficiency, stomatal conductance, and transpiration rate. The nanopollutant upregulated the expression of the histone deacetylase (HDA3) gene and R2R3MYB transcription factor. However, the AP2a gene was down-regulated in response to the PENP treatment. Besides, EPNP epigenetically contributed to changes in DNA methylation. The concentrations of proline, soluble phenols, and flavonoids also displayed an upward trend in response to the applied PENP treatments. The long-term exposure of seedlings to PENP influenced fruit biomass, firmness, ascorbate, lycopene, and flavonoid content. These findings raise concerns about the hazardous aspects of PENP to agricultural ecosystems and food security.

The widespread reliance on single-use plastics (SUPs) has fostered a global throwaway culture, especially in the food packaging industry, where convenience and low cost have driven their adoption, posing serious environmental threats, particularly to marine ecosystems and biodiversity. Edible and ecofriendly packaging made from millet, specifically sorghum (Sorghum bicolor (L.) Moench), is a promising solution to mitigate SUP consumption and promote sustainability. This study explores the development of edible sorghum bowls, enhanced through roasting and incorporating 3 g of hibiscus and rose flower powders. The standardized sorghum bowl was analyzed for nutritional value; optical, technological, functional, and mechanical properties; and shelf life, and the results were discussed. The bowls, 18.5 g of average weight, dimensions of 10.2 cm, and a thickness of 3 mm, were baked in a unique bowl-shaped mold at 80 degrees C for 7 min. Enhancing the bowls with flower powder improved their optical properties and nutrient content. The addition of flower powder also increased phytochemical levels, according to qualitative analysis, while roasting sorghum reduced tannin and phytic acid content. The IC50 values revealed that hibiscus (47.74 mg/mL) and rose (39.87 mg/mL) enrichment boosted antioxidant activity. Sensory evaluations favored roasted bowls across all attributes, while Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analyzer (TGA) analyses confirmed significant structural changes. The enhanced bowls exhibited greater hardness and hold hot or cold snacks for 90 min without compromising structural integrity. Additionally, these bowls demonstrated an extended shelf life, low microbial count (1 x 101CFU/g), reduced toxicity (3%-10% mortality in brine shrimp assays), and complete biodegradation within 15 days in wet soil. These findings indicate that sorghum-based edible bowls present a nutritious, viable, less toxic alternative to SUPs, appealing to a broad demographic, especially in the food and tourism sector, and contributing to environmental conservation by reducing plastic waste and suitable for wide consumption.

Certain Aspergillus spp. release harmful byproducts known as aflatoxins. These carcinogenic toxins contaminate crops, such as groundnut, maize, and rice. This contamination poses a significant health risk and economic burden. Current control methods have limitations. This review explores the potential of silver nanoparticles (AgNPs) as a novel strategy to mitigate aflatoxin contamination (AC). The review highlights the advantages of AgNPs, such as (1) antimicrobial properties against Aspergillus flavus and Aspergillus parasiticus, the aflatoxin producers; (2) effectiveness at concentrations that do not inhibit fungal growth, potentially reducing aflatoxin production; and (3) potential for eco-friendly synthesis using plant extracts. The review also discusses the potential drawbacks of AgNPs viz. (a) environmental concerns regarding accumulation and impact on beneficial soil microbes; and (b) cytotoxicity towards various organisms, requiring further research on safety. Studies suggest AgNPs can inhibit aflatoxin synthesis by disrupting the transcription of aflatoxin biosynthesis genes, damaging the fungal cell membrane and causing leakage of cellular components, and interfering with the secondary metabolism pathway. The review concludes that AgNPs offer a promising approach for aflatoxin control. However, further research is needed to address cytotoxicity concerns and optimise their safe and effective application in agricultural settings.

Global water scarcity entailed the use of treated wastewater (TWW) in agriculture, however, this water can vehiculate numerous pollutants into soil and further crops such as microplastics (MPs). To date, few studies had quantified the accumulation of MPs in soils and earthworms after irrigation with TWW as well as their toxicological effects. Hence, the main objective of the present work is to evaluate the toxicity of MPs using Lumbricus sp. earthworms collected from TWW irrigated soils with an increasing gradient of time (5 years, 16 years and 24 years). MPs determination in soil, as well as in earthworms were performed. The intestinal mucus was quantified, and cytotoxicity (Lysosomal membrane stability (LMS), Catalase (CAT) and glutathione-S-Transferase (GST) activities), neurotoxicity (Acetylcholinesterase activity (AChE)) and genotoxicity (Micronuclei frequency (MNi)) biomarker were assessed. Our results revealed that the use of TWW rendered MPs accumulation in earthworms' tissues and induce alteration on the intestinal mucus. An important cytotoxicity time-depending was observed being associated with an increase on genotoxicity. Overall, the present investigation highlights the ecotoxicological risk associated with the use of TWWs as an important driver of MPs and consequently measures are necessary to reduce MPs in wastewater treatment plans to improve this non-conventional water quality.

Chromium (Cr) poses a high ecological risk, however the toxic mechanisms of Cr in different valence states to soil organisms at cellular and molecular levels are not exactly. In this study, the Eisenia fetida coelomocytes and Cu/ Zn-superoxide dismutase (Cu/Zn-SOD) were chosen as the target subjects to investigate the effects and mechanisms of cellular toxicity induced by Cr(VI) and Cr(III). Results indicated that Cr(VI) and Cr(III) significantly reduced the coelomocytes viability. The level of reactive oxygen species (ROS) was markedly increased after Cr (VI) exposure, which finally reduced antioxidant defense abilities, and induced lipid peroxidation and cellular membrane damage in earthworm coelomocytes. However, Cr(III) induced lower levels of oxidative stress and cellular damage with respect to Cr(VI). From a molecular perspective, the binding of both Cr(VI) and Cr(III) with Cu/Zn-SOD resulted in protein backbone loosening and reduced beta-Sheet content. The Cu/Zn-SOD showed fluorescence enhancement with Cr(III), whereas Cr(VI) had no obvious effect. The activity of Cu/Zn-SOD continued to decrease with the exposure of Cr. Molecular docking indicated that Cr(III) interacted more readily with the active center of Cu/Zn-SOD. Our results illustrate that oxidative stress induced by Cr(VI) and Cr (III) plays an important role in the cytotoxic differences of Eisenia fetida coelomocytes and the binding of Cr with Cu/Zn-SOD can also affect the normal structures and functions of antioxidant defense-associated protein.

The microplastics and organic additives formed in routine use of plastic takeaway food containers may pose significant health risks. Thus, we collected plastic containers made of polystyrene, polypropylene, polyethylene terephthalate, polylactic acid and simulated two thermal usages, including hot water (I) and microwave treatments (M). Nile Red fluorescence staining was developed to improve accurate counting of microplastics with the aid of TEM and DLS analysis. The quantity of MPs released from thermal treatments was determined ranging from 285.7 thousand items/cm(2) to 681.5 thousand items/cm(2) in containers loaded with hot water with the following order: IPS>IPP>IPET>IPLA, while microwave treatment showed lower values ranging from 171.9 thousand items/cm(2) to 301.6 thousand items/cm(2). In vitro toxicity test using human intestinal epithelial Caco-2 cells indicated decrease of cell viability in raw leachate, resuspended MPs and supernatants, which might further lead to cell membrane rupture, ROS production, and decreased mitochondrial membrane potential. Moreover, the leachate inhibited the expression of key genes in the electron transport chain (ETC) process, disrupted energy metabolism. For the first time, we isolate the actually released microplastics and organic substances for in vitro toxicity testing, and demonstrate their potential impacts to human intestine.

Contact herbicides are widely used if rapid weeds eradication is required despite of a number of inherent disadvantages (transfer to water and soil, damage of non-target plants, promotion of resistant weeds expansion, etc.). Supramolecular chemistry can solve the problems associated with herbicide degradation and spreading, as well as suppress their harmful effects on humans and the environment. Pillar[n]arene derivatives are of special interest among other macrocyclic platforms due to their ability to implement various substrates in the macrocycle cavity. However, most of the works devoted to the interaction of pillararenes with pesticides considers binding of paraquat and its derivatives. In this work, water soluble derivatives of pillar[5]arene containing Ltryptophan residues have been proposed for binding a range of herbicides including paraquat dichloride, pyridate, 3-(methylphosphinico)propionic acid, and glufosinate-ammonium. The ester derivative of pillar[5]arene was found to be able to bind the above species. The betaine derivative showed selective and efficient interaction with pyridate (logKa = 4.02) and paraquat (logKa = 3.17). The effect of the charge of the pillar[5]arene substituent on the toxicity of the macrocyclic platform towards A549 and LEK cell lines was demonstrated. Introduction of carboxylate functions to form betaine fragments compensated for the positive charge of the macrocycle substituent and decreased its toxicity by three orders of magnitude for A549 cell line (167.0 mu M), and by two orders of magnitude for LEK cells (56.0 mu M) compared to ester derivative of pillar[5]arene (3.1 and 3.6 mu M respectively). The results obtained confirmed the prospects of the use of amino acid derivatized pillar[5] arenes in the development of new approaches to the removal of the herbicides from the environment that are demanded both in agriculture and aquaculture.

The human health risk assessment through the dermal exposure of metal (loid)s in dust from low latitude and high geological background plateau cities was largely unknown. In this study, the road dust samples were harvested from a typical low-latitude plateau provincial capital city Kunming, Southwest China. The total concentration and dermal bioaccessibility of heavy metal (loid)s in road dust were determined, and their health risks as well as cytotoxicity on human skin keratinocytes were also assessed. The average concentrations of As (28.5 mg/kg), Cd (2.65 mg/kg), Mn (671 mg/kg), and Zn (511 mg/kg) exceeded the soil background values. Arsenic had the highest bioaccessibility after 2 h (3.79%), 8 h (4.24%), and 24 h (16.6%) extraction. The dermal pathway when bioaccessibility is considered has a higher hazard quotient than the conventional method using total metal(loid)s in the dust. In addition, toxicological verification suggested that the dust extracts suppressed the cell viability, increased the reactive oxygen species (ROS) level and DNA damage, and eventually activated the mitochondria-mediated apoptosis pathway, evidenced by the upregulation of Caspase-3/9, Bax, and Bak-1. Cadmium was positively correlated with the mRNA expression of Bax . Taken together, our data indicated that both dermal bioaccessibility and cytotoxicity should be considered for accurate human skin health risk assessment of heavy metal(loid)s in road dust, which may provide new insight for accurate human health risk assessment and environmental management.

Nowadays, animal manure composting constitutes a sustainable alternative for farmers to enhance the level of nutrients within soils and achieve a good productivity. However, pollutants may be present in manures. This study focuses on the detection of environmental microplastics (EMPs) into composts, as well as on the assessment of their potential toxicity on the earthworm Eisenia andrei . To these aims, animals were exposed to two types of compost, namely bovine (cow) and ovine (sheep) manure, besides to their mixture, for 7 and 14 days. The presence and characterization of EMPs was evaluated in all the tested composts, as well as in tissues of the exposed earthworms. The impact of the tested composts was assessed by a multi -biomarker approach including cytotoxic (lysosomal membrane stability, LMS), genotoxic (micronuclei frequency, MNi), biochemical (activity of catalase, CAT, and glutathione- S -transferase, GST; content of malondialdehyde, MDA), and neurotoxic (activity of acetylcholinesterase, AChE) responses in earthworms. Results indicated the presence of high levels of EMPs in all the tested composts, especially in the sheep manure (2273.14 +/- 200.89 items/kg) in comparison to the cow manure (1628.82 +/- 175.23 items/kg), with the size <1.22 mu m as the most abundant EMPs. A time -dependent decrease in LMS and AChE was noted in exposed earthworms, as well as a concomitant increase in DNA damages (MNi) after 7 and 14 days of exposure. Also, a severe oxidative stress was recorded in animals treated with the different types of compost through an increase in CAT and GST activities, and LPO levels, especially after 14 days of exposure. Therefore, it is necessary to carefully consider these findings for agricultural good practices in terms of plastic mitigation in compost usage, in order to prevent any risk for environment health.