Poly(butylene adipate-co-terephthalate) (PBAT) is a promising polymer with excellent mechanical properties and biodegradability. However, knowledge gaps between its degradation and mineralization processes in soil hampers its environmental impact and application potential. In this study, we elucidated the degradation process of PBAT, starting with the degradation of high-molecular-weight polymers into 30 intermediates, before ultimately mineralized into CO2. Bacteria and fungi drove the degradation and mineralization of these intermediates. We discovered that PBAT was synergistically degraded by combinations of 27 bacterial and fungal biomarkers rather than by single biomarkers dominated by Bacteroidota, Acidobacteriota, and Ascomycota. These combinations of related functional genes perform various functions at every stage of PBAT degradation, including breaking down molecular structures, degrading intermediates, and mineralization. Bacterial biomarkers showed greater diversity than fungal biomarkers in degrading PBAT. Our findings provide useful insights into the degradation of PBAT in soil and a foundation for systematically evaluating and controlling the environmental behavior and safety of PBAT in soil.