Over the past decades, anthropogenic disturbance of geological structures has been significantly documented in Slovakia, mainly driven by the national economy's demand for mining resources. Among these resources, brown coal, primarily mined in the Upper Nitra coal basin in the Prievidza district (Slovakia), has been essential. Mining activities around town of Handlov & aacute;, and villages of Ko & scaron;, C & iacute;ge & lcaron; and Sebedra & zcaron;ie, particularly at the C & iacute;ge & lcaron; coal mine, have induced several geological defects. These defects, characterised by large cracks and local landslides, disrupt the hydrogeological conditions, significantly impacting the soil water regime stability of the forest ecosystems in these damaged areas. This study investigates the variability and dynamics of the soil water potential in a mining-affected site (Ra & ccaron;kov laz) compared to an intact reference area (& Ccaron;ertove chodn & iacute;ky) between 2020 and 2022. Our findings suggest that mining activities could have substantial implications for the soil water regime and, consequently, the ecological stability of forest ecosystems.

Biotic and abiotic stresses have emerged as major constraints to agricultural production, causing irreversible adverse impacts on agricultural production systems and thus posing a threat to food security. In this study, a new strain of Bacillus subtilis DNYB-S1 was isolated from soil contaminated with Fusarium wilt. It was found that artificially synthetic flora (YJ-1) [Enterobacter sp. DNB-S2 and Rhodococcus pyridinovorans DNHP-S2, DNYB-S1] could effectively mitigate both biotic (Fusarium wilt) and abiotic (phthalates) sources of stresses, with the inhibition rate of YJ-1 resistant to wilt being 71.25% and synergistic degradation of 500 mg/L PAEs was 91.23%. The adaptive difference of YJ-1 was 0.59 and the ecological niche overlap value was -0.05 as determined by Lotka-Volterra modeling. These results indicate that YJ-1 has good ecological stability. The major degradation intermediates included 2-ethylhexyl benzoate (EHBA), phthalic acid (PA), diisobutyl phthalate (DIBP), and butyl benzoate, suggesting that YJ-1 can provide a more efficient pathway for PAEs degradation. In addition, there was metabolic mutualism among the strains that will selectively utilize the provided carbon source (some metabolites of PAEs) for growth. The pot experiment showed that YJ-1 with cucumber reduced the incidence of cucumber wilt by 45.31%. YJ-1 could reduce the concentration of PAEs (DBP: DEHP = 1:1) in soil species from 30 mg/kg to 4.26 mg/kg within 35 d, with a degradation efficiency of 85.81%. Meanwhile, the concentration of PAEs in cucumber was reduced to 0.01 mg/kg, indicating that YJ-1 is directly involved in the degradation of soil PAEs and the enhancement of plant immunity. In conclusion, this study provides a new perspective for the development of customized microbiomes for phytoremediation under combined biotic-abiotic stresses in agricultural production processes.