An analytical methodology was developed for the first time in this work enabling the simultaneous enantiomeric separation of the fungicide fenpropidin and its acid metabolite by Capillary Electrophoresis. A dual cyclodextrin system consisting of 4 % (w/v) captisol with 10 mM methyl-beta-cyclodextrin was employed in a 100 mM sodium acetate buffer at pH 4.0. Optimal experimental conditions (temperature 25 degrees C, separation voltage -25 kV, and hydrodynamic injection of 50 mbar x 10 s) allowed the simultaneous separation of the four enantiomers in <10.7 min with resolutions of 3.1 (fenpropidin) and 3.2 (its acid metabolite). Analytical characteristics of the method were evaluated and found adequate for the quantification of both chiral compounds with a linearity range from 0.75 to 70 mg L-1, good accuracy (trueness included 100 % recovery, precision with RSD<6 %), and limits of detection and quantification of 0.25 and 0.75 mg L-1, respectively, for the four enantiomers. No significant differences were found between the concentrations determined and labelled of fenpropidin in a commercial agrochemical formulation. The stability over time (0-42 days) of fenpropidin enantiomers using the commercial agrochemical formulation was evaluated in two sugar beet soils, revealing to be stable at any time in one sample, while in the other a decrease of 45 % was observed after 42 days. Individual and combined toxicity of fenpropidin and its metabolite was determined for the first time for marine organism Vibrio fischeri, demonstrating higher damage caused by parent compound. Synergistics and antagonists' interactions were observed at low and high effects levels of contaminants.

The bank protection measures of waterways shall become more environmentally friendly in the future including the use of plants instead of stones. The low levels of protection provided by plants in the early phase after planting requires a process-based understanding of soil-wave-interaction. One process that is considered essential is liquefaction where the soil undergoes a phase-change from solid-like to fluid-like behaviour which could reduce the safety of the system. The aim of this publication is to analyse the results of column experiments on wave-induced soil liquefaction and to develop a numerical model which is able to describe the entire process from the pre-liquefaction phase to the following reconsolidation in order to support the analysis of liquefaction experiments. Numerical simulations of the column experiments were done using a fully coupled hydro-mechanical model implemented in the open-source software FEniCS. A permeability model derived from granular rheology allows the simulation of dilute as well as dense suspensions and sedimented soil skeletons. The results of the simulations show a good agreement with the experimental data. Theoretical limits in the liquefied state are captured without the common modelling segmentation into pre-and post-liquefaction phase. Due to the modular structure of the implementation, the constitutive setting can be adjusted to incorporate more complex formulations in order to study the influence of wall friction and non-linearity in soil behaviour.

Cadmium (Cd) pollution leads to reduced crop yields and poses a threat to human health, making it an important environmental and agricultural safety issue. Selenium [Se(V)] has been shown to alleviate Cd stress in plants; however, the mechanisms underlying Se-mediated protection against Cd toxicity remain largely unclear. In this study, we investigated the physiological and molecular mechanisms of Se(W)-alleviated Cd toxicity in strawberry plants through physio-biochemical and transcriptomic analyses. Our results showed that foliar spraying with Se (IV) increased photosynthetic efficiency, reduced Cd-induced oxidative damage by enhancing antioxidant enzyme activities and soluble sugar contents, thereby improving Cd stress tolerance. Transcriptomic profiling revealed 477 common differentially accumulated transcripts (DATs), predominantly enriched in transporter activity, oxidoreductase function, and antioxidant-related processes. Notably, seven key genes involved in Cd efflux, chelation, secondary metabolite transport and nutrient uptake (FvPCR9-like, FvCBP-like, FvWATI-like, FvMOT1, FvY1476gO214O, FvNR12.1 and FvZIP8) exhibited opposite expression patterns under Se(W) and Cd treatments. Supplementation with Se(IV) also modulated phytohormone signaling, nitrogen metabolism and carbon metabolism pathways, providing a multi-dimensional approach to mitigating Cd-induced physiological disruptions. This study provides novel insights into Se(IV)-mediated Cd stress adaptation, and offers promising strategies for developing low-Cd-accumulating crops, addressing critical environmental and agricultural challenges associated with heavy metal contamination.

Post-grouted shafts (PGDS) and stiffened deep cement mixed (SDCM) shafts reinforce the surrounding soils with cement to enhance the bearing capacity of shaft foundations, and their applications are becoming increasingly widespread. Field tests involving two post-grouted shafts and two stiffened deep cement mixing shafts were conducted at the bridge foundations projects, analyzing the vertical bearing performance of the shafts with cement-stabilized soil enhancement. Additionally, numerical simulations were performed to establish calculation models for single shaft and groups of drilled shafts, PGDS, and SDCM shafts, enabling a comparative analysis of their bearing capacity performance within the identical strata. The results indicate that the post- grouted shaft demonstrated significant bearing deformation capacity, as confirmed by field tests. After grouting, the ultimate bearing capacities of DS1 and DS2 improved by 124.5 % and 110.9 %, respectively. In both single and group modeling shaft foundations, the post-grouted shafts demonstrated the highest bearing deformation characteristics, followed by the identical- size stiffened deep cement mixed shaft, while the long-core SDCM shafts and the ungrouted shafts exhibited the weakest performance. Due to interaction effects among group shafts, the total bearing capacity of the group shafts is not simply the sum of the individual shafts. Specifically, the reduction factor for group shaft capacity ranges from 0.68 to 0.79 at the Baoying Large Bridge site, while at the Yangkou Canal Bridge site, it varies from 0.66 to 0.85. The findings of this study provide valuable insights for practical engineering applications.

Throughout history, plant diseases have posed significant challenges to agricultural progress, driven by both abiotic and biotic factors. Abiotic factors include wind, salt damage, freezing, girdling roots and compacted soil, while biotic factors encompass bacteria, nematodes, fungi and viruses. Plants have evolved diverse defense strategies to counter pathogen attacks, one of which involves chitinases, a subset of pathogenesis-related proteins. Chitinases are hydrolytic enzymes that degrade chitin, a high-molecular-weight linear polymer of N-acetylD-glucosamine, which is a crucial component of fungal cell walls and septa. These enzymes are produced by a wide range of organisms, including plants, animals, insects, fungi and microorganisms. In plants, chitinases are strongly expressed under pathogenic stress, primarily targeting fungal pathogens by breaking down their cell walls. They also contribute to cell wall remodeling and degradation during growth and defense processes. Numerous studies have demonstrated that the antifungal activity of chitinases is influenced by the chitin concentration and surface microstructure of different fungal species. Research has highlighted their role in protecting plants like mango, cucumber, rye, tomato, grapevine and other plants from various fungal diseases. These findings underscore the critical role of chitinases in plant defense mechanisms, showcasing their importance in mitigating fungal infections and supporting plant health.

This study investigates the potential of green-fabricated manganese dioxide (MnO2) nanoparticles (NPs) to mitigate chromium (Cr) toxicity in wheat, presenting a novel approach to enhancing ion homeostasis and physiological resilience under Cr stress. Chromium contamination in agricultural soils is a significant concern, severely impacting crop productivity and disrupting the physiological homeostasis of wheat. Chromium exposure compromises nutrient uptake, induces oxidative stress, and impairs plant growth and yield. This study explored the use of green-fabricated MnO2NPs to mitigate Cr-induced oxidative stress in two bread wheat cultivars, Borlaug-16 and SKD-1. Seed nano-priming with MnO2NPs (100, 250, and 500 mg kg-1) was applied, followed by Cr (100 mg kg-1) exposure, and key physiological, biochemical, and ionomic responses were evaluated. Manganese dioxide nanoparticles significantly reduced Cr uptake and improved ion transport. In Borlaug-16, NP250 enhanced seedling height by 74 %, while NP100 reduced H2O2and TBARS by 60.28 % and 50.17 %, respectively, indicating improved oxidative stress tolerance. SKD-1 exhibited greater Cr stress tolerance, with NP250 improving root length by 31.03 % and relative water content by 56.66 %, supporting better water retention. Additionally, MnO2NP treatments boosted antioxidant enzyme activities, increasing APX and GPX by up to 12.47 %, and restored root and leaf anatomy, reversing Cr-induced structural damage. Furthermore, MnO2NPs enhanced the uptake of essential nutrients such as calcium, potassium, and magnesium, while restricting Cr translocation, improving overall nutrient efficiency. These findings emphasize the potential of MnO2NPs as an eco-friendly strategy for enhancing crop resilience and promoting sustainable agriculture in Cr-contaminated soils.

Fusarium graminearum poses a major threat to barley production worldwide. While seed priming is a promising strategy to enhance plant defense, the use of unconventional priming agents remains underexplored. This study investigates the protective effects of pre-infection camel urine seed priming on barley seedlings challenged with Fusarium graminearum, focusing on growth, disease resistance, oxidative stress, and defense-related responses. Barley grains were primed with camel urine and grown in both Fusarium-infested and uninfested soils. Fusarium infection initially triggered a sharp increase in oxidative stress markers reflecting an early oxidative burst commonly associated with defense signaling. However, in hydro-primed seedlings, this response persisted, leading to sustained oxidative damage and growth suppression. In contrast, camel urine priming modulated the oxidative burst effectively, initially permitting H2O2 accumulation for defense activation, followed by a rapid decline, resulting in an 84.53 % reduction in disease severity and maintenance of seedling growth under infection. This was accompanied by enhanced antioxidant defenses, as indicated by significantly increased activities of antioxidant enzymes, and a 145 % increase in total antioxidant capacity compared to control. Camel urine priming also showed a reduction in shikimic acid levels under infection, suggesting increased metabolic flux toward the phenylpropanoid pathway. Thus, phenylalanine ammonia-lyase activity, phenolic compounds, and flavonoids were significantly elevated. Antifungal enzymes, beta-glucanase and chitinase, also remained high in camel urine-primed seedlings, in contrast to their sharp decline in hydro-primed controls. These findings highlight camel urine priming as a promising, sustainable approach for managing Fusarium in barley.

Profenofos is one of the most widely used toxic organophosphate insecticides used in crop fields against various insect pests. The pesticide may spread into the environment through various sources, such as air, water, soil, etc. Therefore, there is a high risk of ingestion for animals and aquatic fauna. The present study aimed to assess the profenofos-induced hepatotoxicity, genotoxicity, and haematological abnormalities of the freshwater fish Channa punctatus, which can indicate the probable threat of this pesticide to other species, including humans. Freshwater fish Channa punctatus were exposed to two sub-lethal concentrations of Profenofos (0.078 and 0.157 mg/L). After 7 days of consecutive exposure, changes in haematological parameters, hepatic tissue histology, and genotoxic effects were evaluated. The total count of red blood cells (RBCs), white blood cells (WBCs), and haemoglobin (Hb) % was done. Along with the histological changes in hepatic tissues, genotoxic studies were also carried out. The study showed that profenofos induces different changes in the liver's haematology and histology and nuclear abnormalities in the erythrocytes of treated fish. The results indicate significant differences in RBC and Hb%, whereas marked elevations in WBC count were recorded. The histopathological study of the liver in the treated fish revealed some substantial changes like cell damage, distorted cell shape, vacuolations, etc. Some significant genotoxic effects of profenofos in the erythrocytes were observed, such as the induction of micronuclei, lobed nuclei, irregular-shaped nuclei, notched nuclei, and distorted nuclei. The results were statistically significant at the p < 0.05 level. The study explores the toxic effects of pesticides on the overall health of the fish species. Moreover, the study tried to focus on making decisions about using a tolerable prescribed dose of chemicals to minimize the risk of pesticides. People will also learn that the contamination of the insecticide profenofos is harmful to the aquatic ecosystem, and therefore indiscriminate measures should be avoided.

Cadmium (Cd) contamination in agricultural soils poses a serious threat to crop productivity and food security, necessitating effective mitigation strategies. This study investigates the role of silicon nanoparticles (SiNPs) in alleviating Cd-induced stress in maize (Zea mays L.) under controlled greenhouse conditions. Sterilized maize seeds were sown in sand-filled pots and treated with varying SiNP concentrations (0%, 0.75%, 1.5%, 3%, and 6%) with or without Cd (30 ppm). Physiological, biochemical, and antioxidant parameters were analyzed to assess plant responses. Results demonstrated that SiNPs significantly enhanced photosynthetic pigment concentrations, with chlorophyll-a, chlorophyll-b, and carotenoids increasing by 45%, 35%, and 50%, respectively, in the 6% SiNP + 30 ppm Cd treatment. Biochemical analyses revealed improved osmotic adjustment, as indicated by higher soluble protein (6.52 mg/g FW) and proline (314.43 mu mol/g FW) levels. Antioxidant enzyme activities, including superoxide dismutase, catalase, and ascorbate peroxidase, were markedly higher in SiNP-treated plants, mitigating oxidative damage. Additionally, SiNPs reduced Cd accumulation in plant tissues, suggesting a protective role in limiting metal toxicity. These findings highlight SiNPs as a promising approach for enhancing maize resilience against Cd stress, with potential applications in sustainable agriculture for improving crop health in contaminated soils.

Soil-borne pathogens can severely reduce vegetable crop output and quality. A disease complex may develop when many soil-borne pathogens attack a crop simultaneously, which can cause more damage. The soil-borne fungus Fusarium oxysporum (Fo) and the nematode Meloidogyne incognita (Mi) significantly reduce global tomato (Solanum lycopersicum L.) yields. After a soil-borne pathogenic infection, plants undergo numerous changes. Therefore, we conducted the present study to examine the impact of soil-borne pathogens Fo and Mi on the growth, physiology, biochemical, and root morphology of tomato cultivars Zhongza 09 (ZZ09) and Gailiang Maofen 802 (GLMFA and GLMFB) at 10, 20, and 30 days after-inoculation (DAI). The present study revealed that combined infections adversely damaged plant growth, photosynthetic pigmentation, gas exchange, biochemistry, and root morphology. The plant growth reduction in GLMFA and GLMFB was greater than in ZZ09. The chlorophyll content and photosynthetic indices declined dramatically; however, ZZ09 declined less than GLMFA and GLMFB plants. In GLMFA and GLMFB plants, the combined infection of Fo and Mi lowered plant-defense-related antioxidant activity compared to their single infection or control. ZZ09's antioxidants were greatly up-regulated, indicating pathogen tolerance. ZZ09 had significantly lower gall and wilt disease indices than GLMFA and GLMFB. Moreover, the microscopic examination of roots showed that Fo and Mi infection damaged GLMFA and GLMFB more than ZZ09 plants. Thus, combined infection induced severe root damage, reduced plant growth, reduced antioxidants, and increased reactive oxygen species (ROS) production compared to single inoculation. However, the ZZ09 cultivar exhibited significantly stronger tolerance to combined infection.