In green onion harvesting, the problems of easy dumping and low rate of clean digging can be encountered. In this paper, a kind of harvesting device for digging and pulling green onions, referred to simply as the device, was designed. The device mainly consists of a digging shovel, screen bars, clamping conveyor belt, etc. This paper focuses on the analysis of the model forces of green onions and soil in the two states of the onion digging process without dumping and clamping. The key factors affecting the model state of onions and soil were identified as: screen bar length l(2), screen bar inclination angle beta, and pulling point position x. Based on the discrete element simulation technology of EDEM, the mechanism-crop-soil model was established, and a single-factor simulation test was conducted to determine the range of values for each factor. Taking the advantages of field test and three-factor five-level orthogonal experimental design, the parameter combinations of green onion harvesting operation evaluation indices were optimized, including a pulling point position of 166 mm, screen bar length of 242 mm, and screen bar inclination angle of 14 degrees. As the results of the field test show, the harvester operation was stable without congestion or damage, the harvesting effect of green onions was improved, and the clean digging rate reached 100%, which meets the agronomic requirements for onion harvesting and the expectations of users.
Cadmium (Cd) is a highly toxic agricultural pollutant that inhibits the growth and development of plants. Arbuscular mycorrhizal fungi (AMF) can enhance plant tolerance to Cd, but the regulatory mechanisms in Allium fistulosum (green onion) are unclear. This study used a Cd treatment concentration of 1.5 mg center dot kg-1, which corresponds to the risk control threshold for soil pollution in Chinese agricultural land, to examine the effects and molecular mechanisms of AMF inoculation on the growth and physiology of green onion under Cd stress. AMF formed an effective symbiotic relationship with green onion roots under Cd stress, increased plant biomass, improved root structure and enhanced root vitality. AMF-colonized green onion had reduced Cd content in roots and leaves by 63.00 % and 46.50 %, respectively, with Cd content being higher in the roots than in the leaves. The ameliorative effect of AMF on Cd toxicity was mainly due to a reduction in malondialdehyde content in leaves (30.12 %) and an enhancement of antioxidant enzyme activities (peroxidase, catalase, superoxide dismutase, glutathione reductase and reduced glutathione) that mitigated damage from excessive reactive oxygen species. In addition, AMF induced secretion of easily extractable glomalin soil protein and total glomalin-related soil protein and inhibited the translocation of Cd to the shoots. Transcriptomic and metabolomic correlation analyses revealed that differentially expressed genes and metabolites in AMF-inoculated green onion under Cd stress were predominantly enriched in the phenylpropanoid biosynthesis and phenylalanine metabolism pathways, upregulated the expression of the HCT, PRDX6, HPD, MIF, and HMA3 genes, and accumulation of the phenylalanine, L-tyrosine, and 1-O-sinapoyl-beta-glucose metabolites. Thus, AMF enhance Cd tolerance in green onions by sequestering Cd in roots, restricting its translocation, modulating antioxidant defenses and inducing the expression of genes involved in the phenylpropanoid biosynthesis and phenylalanine metabolism pathways. Collectedly, we for the first time revealed the mechanism of AMF alleviating the toxicity of Cd to green onion, providing a theoretical foundation for the safe production and sustainable cultivation of green onion in Cdcontaminated soils.