The water and energy in the land surface and lower atmosphere have a strong coupling relationship. Apart from the land surface temperature (Ts) and air temperature (Ta), the land surface-air temperature difference (Ts-Ta) is also an essential parameter reflecting the coupling process. However, the global spatiotemporal variations and influencing factors of Ts-Ta remain not well explored. Here, ERA5-land reanalysis data, GIMMS NDVI data, and elevation data were used to analyze the global spatiotemporal heterogeneity and influencing factors of Ts-Ta. It was found that annual mean Ts-Ta exhibited a decreasing trend from the equator to polar areas. And the annual Ts-Ta increased at 0.009 degrees C/ 10a from 1981 to 2020. The variations of global net radiation mainly determined the spatiotemporal heterogeneity of global Ts-Ta. The different properties of the land surface and near-surface atmosphere were the main factors affecting the Ts-Ta, including soil moisture, vegetation, snow cover, and the water vapor content in the atmosphere. In addition, Ts and Ta also affected each other. These findings are conducive to a better understanding of the land-atmosphere coupling, and it is of great significance to take better measures to adapt the global climate change.