Greece's olive oil production is significantly affected by the olive fruit fly Bactrocera oleae (Diptera: Tephritidae), and its presence is perceived when it is too late to act for damage recovery. In this work, some unexplored entomopathogenic fungi (EPFs) were studied for their efficacy on olive fruit fly pupae in soil samples. Olive grove soil samples were collected to evaluate the effect of EPFs in their natural environment. The parameters that were analyzed to evaluate the performance of EPFs on B. oleae included the adult survival time, pupa hatch time, and the presence of mycelium on B. oleae pupae and dead adults. The efficacy of some EPFs was highlighted by the mycelium present on dead B. oleae adults after treating pupae with fungal isolates on the soil substrate. The results showed that for the soil substrate, external fungal growth was observed in dead adults with A. contaminans, A. keveii, A. flavus P. lilacinum, and T. annesophieae (100%). Remarkably, the lowest male proportion for soil and non-soil substrates was for A. flavus (0.41-0.42) for the first time, for A. keveii (0.36), and for P. citreosulfuratum (0.41) on the soil-only substrate in contrast to the control treatment (0.5 for both substrates). Given the high infestation caused by the olive fruit flies in Greece, the results of the study emphasize to use of incorporating certain EPF-based biopesticides into integrated pest management (IPM) programs.

The heterogeneity of a dense sand specimen in triaxial compression has been revealed in many different studies using tools such as x-ray computed tomography. It has been shown that a significant variation of the soil variables already exists at the initial state and that, if shear banding occurs, all variables localise inside the specimen. To resolve the discrepancy between such observations and the assumption of a homogeneous specimen, which is commonly made in the interpretation of triaxial tests, one could either extract the local soil behaviour rather than the global one or use the initial distribution of the variables as the initial state of a boundary value problem. For both purposes, the size of a representative elementary volume (REV) is determined regarding the void ratio, two contact fabric descriptors, the volumetric and deviatoric strain. The size of the REV is either determined for individual loading states or by considering the evolution of deforming elements throughout the triaxial test. At the final loading state, a REV size of 3.6 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$d_{50}$$\end{document} is identified, which is also the size where the statistical distribution of the variables becomes independent of the element size. The same size is determined for the deforming elements and is therefore used to extract the soil behaviour from the evolving shear band. The local soil behaviour is found to be much simpler than the global one, which suggests that the complexity of the global behaviour mainly results from homogenising the highly different zones inside the specimen.Graphical AbstractExtraction of the soil behaviour inside the evolving shear band with the help of deforming representativeelementary volumes. The volumetric behaviour is represented by the evolution of the void ratio and the evolution ofthe contact fabric anisotropy is closely connected to the stress-strain behaviour. The soil behaviour on the REVscale might form the basis for an alternative approach for the development and calibration of constitutive modelsconsidering the heterogeneity of a soil specimen.