Organophosphate pesticides, widely used in agriculture, are effective in pest control but pose environmental and health risks through soil, water, and air contamination. Exposure to these chemicals is linked to adverse human health effects, underscoring the need for environmentally sustainable practices. This study aimed to assess urinary organophosphate metabolites and examine the relationship between GSTM1 and GSTT1 gene polymorphisms with biomarkers of oxidative stress among farmers in Himachal Pradesh exposed to pesticides. We collected urine samples (50 mL) from the exposed group to detect organophosphate metabolites using GC-MS. Blood samples (5 mL) were also obtained for GSTM1 and GSTT1 genotyping and assessment of antioxidant enzyme activities. The results showed decreased enzymatic activity of SOD (2.92 +/- 1.07) and catalase (12.60 +/- 3.15) in the exposed group, with increased MDA levels (4.14 +/- 1.36), compared with the unexposed group (SOD: 7.04 +/- 1.34, catalase: 25.75 +/- 2.20, MDA: 1.15 +/- 0.18). No significant associations (p > .05) were found between GSTM1 or GSTT1 genotypes and SOD, catalase, or MDA activities. The study concluded that prolonged pesticide exposure induces oxidative stress linked to specific genetic variations, suggesting directions for further research into the toxicogenetics of pesticide exposure and its health implications.

The use of plant growth-promoting microorganisms is an effective agricultural practice to improve plant growth, especially under abiotic stress. In this study, the combined impact of three plant growth-promoting bacteria (PGPB) namely Brevibacterium halotolerans (Sd-6), Burkholderia cepacia (Art-7), Bacillus subtilis (Ldr-2) were tested with Trichoderma harzianum (Th) (possessing ACC deaminase producing activity) in Ocimum basilicum L. cv. Saumya to reduce drought-induced damages to the plants under different level of drought stress [i.e. wellwatered (100 %), moderate (60 %), severe (40 %)]. These PGPB strains, along with Th, were found to be tolerant against osmotic stress when tested in growth media containing different concentrations of polyethylene glycol (PEG 8000), and all were found to endure -0.99 MPa water potential. Compared to non-inoculated control, Th+Ldr-2 treatment improved fresh herb weight (62.45 %) and oil content (61.54 %) and higher photosynthetic rate under severe drought. Besides, in relation to control, the above treatment enhanced nutrient uptake, reduced ABA, ACC as well as ethylene levels and increased IAA content in addition to an increase in important constituents of essential oil, indicating better performance in terms of plant growth under drought. Higher RWC, decreased MDA, and reduced antioxidant activities in Th+Ldr-2 treated plants compared to non-inoculated control under drought support the mechanism of the microbes providing tolerance against drought. Colony forming unit of microbes and scanning electron microscopy (SEM) study support the effective colonisation behaviour of Th+Ldr-2, which protects plants against drought stress. A consortium of diverse microbes, found to improve plant growth under drought through increased nutrient uptake, reducing the levels of ACC and ABA, improving the content of IAA, antioxidant enzymes probably reducing the effect of drought stress and improving plant biomass could be a useful tool to reduce drought-induced losses in crop plants.

The current study was conducted on the inhabitants living in the area adjacent to the Hudiara drain using bore water and vegetables adjacent to the Hudiara drain. Toxic heavy metals badly affect human health because of industrial environmental contamination. Particularly hundreds of millions of individuals globally have faced the consequences of consuming water and food tainted with pollutants. Concentrations of heavy metals in human blood were elevated in Hudiara drainings in Lahore city, Pakistan, due to highly polluted industrial effluents. The study determined the health effects of high levels of heavy metals (Cd, Cu, Zn, Fe, Pb, Ni, Hg, Cr) on residents of the Hudiara draining area, including serum MDA, 8-Isoprostane, 8-hydroxyguanosine, and creatinine levels. An absorption spectrophotometer was used to determine heavy metals in wate water, drinking water, soil, plants and human beings blood sampleas and ELISA kits were used to assess the level of 8-hydroxyguanosine, MDA, 8-Isoprostane in plasma serum creatinine level. Waste water samples, irrigation water samples, drinking water samples, Soil samples, Plants samples and blood specimens of adult of different weights and ages were collected from the polluted area of the Hudiara drain (Laloo and Mohanwal), and control samples were obtained from the unpolluted site Sheiikhpura, 60 km away from the site. Toxic heavy metals in blood damage the cell membrane and DNA structures, increasing the 8-hydroxyguanosine, MDA, creatinine, and 8-Isoprostane. Toxic metals contaminated bore water and vegetables, resulting in increased levels of creatinine, MDA, Isoprostane, and 8-hydroxy-2-guanosine in the blood of inhabitants from the adjacent area Hudiara drain compared to the control group. In addition,. This study also investigated heavy metal concentrations in meat and milk samples from buffaloes, cows, and goats. In meat, cow samples showed the highest Cd, Cu, Fe and Mn concentrations. In milk also, cows exhibited elevated Cu and Fe levels compared to goats. The results highlight species-specific variations in heavy metal accumulation, emphasizing the need for targeted monitoring to address potential health risks. The significant difference between the two groups i.e., the control group and the affected group, in all traits of the respondents (weight, age, heavy metal values MDA, 8-Isoprostane, 8-hydroxyguaniosine, and serum creatinine level). Pearson's correlation coefficient was calculated. The study has shown that the level of serum MDA, 8-Isoprostane, 8-hydroxyguaniosine, or creatinine has not significantly correlated with age, so it is independent of age. This study has proved that in Pakistan, the selected area of Lahore in the villages of Laloo and Mohanwal, excess of heavy metals in the human body damages the DNA and increases the level of 8-Isoprostane, MDA, creatinine, and 8-hydroxyguaniosine. As a result, National and international cooperation must take major steps to control exposure to heavy metals.

This paper presents a novel approach to examining the impact of soil settlement and its spatial distribution on infrastructure. The study focuses on a specific road in the Friesland coastal plain in the north of the Netherlands, investigating how the Holocene coastal subsurface architecture influences settlement patterns. Our study underscores the importance of integrating multiple datasets, providing data at varying resolutions. The road segment traverses lithostratigraphical units, which include tidal channel and tidal flat deposits, overlaying an older tidal basin system and intercalated peat beds. Through data assimilation of a settlement model optimized with InSAR measurements, we have identified settlement heterogeneities that can be explained by combining high-resolution variations in lithology with gradual changes in lithostratigraphy. This was accomplished by utilizing a medium-resolution model (MRM) based on borehole descriptions and a high-resolution data (HRD) approach based on cone penetration tests along the road. The HRD method proved more effective in capturing abrupt changes in lithology between channel structures, while the MRM provided a continuous representation of the lithostratigraphic setting of the area. Our study demonstrates that subsurface heterogeneities have significant implications for subsidence along roads. Settlement rates increase from 2-4 mm/year towards 9 mm/year along the road section, resulting in a differential settlement of more than 5 mm/year over a distance of less than a kilometer. This is primarily attributed to variations in lithostratigraphy. Overall, this new innovative approach offers a practical and cost-effective solution for predicting subsidence due to settlement, eliminating the need for expensive laboratory tests. By integrating lithology and lithostratigraphy, more efficient road maintenance and management become possible.