Studying permafrost changes under different (e.g., glacial/interglacial) and changing (e.g., current various scenarios) climates can potentially advance our understanding of permafrost's responses to climate change and further enable informed policy making for mitigating impacts from permafrost changes. Despite existing studies generally focusing on permafrost change during certain periods, here, we have synthetically examined the changes of the Northern Hemisphere near-surface permafrost during the six periods (Last Glacial Maximum (LGM, similar to 21 ka), mid-Holocene (MH, similar to 6 ka), preindustrial (PI, ca 1850), future 1.5 degrees C and 2.0 degrees C global warming periods, and end of the 21st century), using the surface frost index (SFI) model and outputs of six climate models. Simulated climate anomalies plus present-day observed climatology are used to drive the SFI model in this study. This helps correct systematic biases in permafrost change simulations.The results show that multi-model ensemble extent of present-day near-surface permafrost in the Northern Hemisphere agree well with the observations, with an area bias of 0.27x106 km2 in area (1.8% of the total observed area). Minor deviations (1.55x106 km2) in the simulated present-day permafrost extents across the climate models indicate a low inter-model diversity. In response to changes in annual mean surface air temperature of -10.3 +/- 2.3 degrees C (LGM), +0.1 +/- 0.5 degrees C (MH), +2.6 +/- 0.7 degrees C (1.5 degrees C global warming, RCP4.5), +3.6 +/- 1.0 degrees C (2.0 degrees C global warming, RCP4.5), and +5.0 +/- 1.3 degrees C (end of the 21st century, RCP4.5) in present-day permafrost regions relative to the PI, the changes in near-surface permafrost area are +33%+/- 30% (LGM), -13%+/- 6% (MH), -25%+/- 8% (1.5 degrees C warming, RCP4.5), -35% +/- 10% (2.0 degrees C warming, RCP4.5), and -55%+/- 12% (end of the 21st century, RCP4.5), respectively. From the LGM to the future, near-surface permafrost extent substantially decreases, underlining its high sensitivity to climate change and implying its potentially profound impacts in a warming future.

The use of various sustainable materials and cement is a frequent and successful strategy for stabilizing problematic soil. The current research discusses the potential use of discarded millet husk ash (MHA) and cement (C) as subgrade ingredients to improve the geotechnical qualities of soil (S). MHA and cement are mixed in different proportions and the engineering characteristics of the stabilized soil are studied. The study involves examining fundamental properties, such as specific gravity and Atterberg's limits, as well as engineering properties, including Unconfined Compressive Strength (UCS) and California Bearing Ratio (CBR) tests. These evaluations are conducted to assess the feasibility of using the MHA-cement blend as a construction material. Additionally, FTIR & SEM analysis shows the addition of MHA-cement blend effectively couples with the soil. The test findings demonstrate that adding MHA to soil lead to decreased liquid limits and plasticity indices. The maximum dry density (MDD) was observed to decrease when MHA was mixed with soil. When 8% cement was incorporated to the S:MHA (84.5:7.5) combination, the UCS value rose even higher reaching 1600.1 kPa. The S:MHA:C arrangement in the ratio of 84.5:7.5:8 had the greatest California bearing ratio (CBR). Fourier transform infrared spectroscopy (FTIR) elucidated the various types of bond formations present within the soil composite and deeper peaks depicted greater presence of cementitious compounds after curing period. SEM analysis exhibited a greater density of N-A-S-H and C-A-S-H gels in comparison to natural soil samples. The findings suggest that the MHA-cement blend can effectively enhance the geotechnical properties of problematic soils, while addressing issues of agricultural waste management. This research contributes to several Sustainable Development Goals (SDGs), including SDG 9 (Industry, Innovation, and Infrastructure) by promoting innovative construction materials.

Despite its proven high toxicity, unsymmetrical dimethylhydrazine (UDMH) continues to be used in rocket technology and some other areas of human activity. In this work, the ability of plant-bacterial consortia to reduce the genotoxicity of UDMH and its incomplete oxidation products was investigated. Genotoxicity was assessed using a specific lux-biosensor, Escherichia coli MG1655 pAlkA-lux, which emits stronger light when cellular DNA is alkylated. For microbiological biodegradation, the Bacillus subtilis KK1112 strain was isolated from the soil via a two-stage selection process for resistance to high UDMH concentrations exceeding 5000 MAC. This strain's ability to biodegrade UDMH was demonstrated, as treatment of UDMH-polluted medium with KK1112 resulted in reduced DNA alkylation. A synergistic reduction in the DNA-alkylating potency of UDMH oxidation products was studied under the combined application of bacteria KK1112 and plant seedlings: Bromus inermis Leyss, Medicago varia Mart. and Phleum pratense L. The greatest effect was achieved when bacteria were used in combination with B. inermis. KK1112 cells accelerated seedling development and mitigated UDMH-induced growth inhibition. The findings suggest that the consortium of KK1112 and B. inermis has a great potential for remediation of UDMH-polluted soils in arid climatic zones.

The present study proposes a rapid visual screening methodology for multi-hazard vulnerability assessment (termed as MH-RVS) of reinforced concrete (RC) buildings in the Indian Himalayan region considering earthquakes, debris flow, debris flood, and soil subsidence. An extensive field survey of 1200 buildings was conducted in three hill towns situated in the Northwestern Indian Himalayan region to identify prevalent multi-hazard vulnerability attributes. The presented MH-RVS methodology is statistically developed based on the information obtained from the current field survey and existing post-hazard reconnaissance studies. The proposed methodology effectively addresses the concern of underpredicting the expected damage states of RC buildings situated in hilly regions subjected to multi-hazard scenarios when they are assessed using RVS methodologies of seismic vulnerability assessment. Further, a simplified MH-RVS form is developed to collect field data and conveniently segregate the RC buildings based on their expected damage state under multi-hazard scenarios involving earthquakes, debris flow, debris flood, and soil subsidence. Stakeholders and decision-makers can use the proposed MH-RVS methodology to assess the perceived vulnerability of RC buildings in the Indian Himalayan region and devise timely strategies for structural strengthening and risk mitigation.

Soil salinization is one of the major environmental factors that restrict plant growth and development. Zeaxanthin epoxidase (ZEP) functions in ABA biosynthesis and the xanthophyll cycle and has a vital role in plant responses to various environmental stresses. It was found by quantitative real-time PCR (qRT-PCR) that MhZEP responded to saline-alkali stress and showed the highest expression at 48 h of saline-alkali stress, which was 14.53-fold of 0 h. The MhZEP gene was cloned from the apple rootstock begonia (Malus halliana Koehne) and its protein physicochemical properties were analyzed. Subsequently, the functional characterization of MhZEP (ID: 103403091) was further investigated in Arabidopsis thaliana. The MhZEP contained a complete open reading frame with a length of 1998 bp, and encoded 665 amino acids with an isoelectric point of 7.18. Phylogenetic tree analysis showed that MhZEP was the most homologous and closely related to Glycine max. Compared with wild-type, transgenic plants grew better under saline-alkali stress and the MhZEP-OE line showed higher chlorophyll content, carotenoid content, enzyme activities (POD, SOD, CAT and APX) and K+ content, whereas they had lower chlorosis and Na(+ )content than the wild type (WT), which indicated that they had strong resistance to stress. The expression levels of saline-alkali stress-related genes in A. thaliana MhZEP-OE were examined by qRT-PCR, and it was found that the MhZEP improved the tolerance of A. thaliana to saline-alkali stress tolerance by regulating the expression of carotenoid synthesis genes (MhPSY, MhZDS, MhLYCB and MhVDE) and ABA biosynthesis genes (MhNCED5, MhABI1 and MhCYP707A2). And the potassium-sodium ratio in the cytoplasm was increased to maintain ionic homeostasis by modulating the expression of Na+ transporter genes (MhCHX15 and MhSOS1) and K+ transporter genes (MhHKT1;1, MhNHX1 and MhSKOR1). Moreover, the expression of H+-ATPase genes (MhAHA2 and MhAHA8) was increased to reduce the oxidative damage caused by saline-alkali stress. In summary, MhZEP acted as an essential role in plant resistance to saline-alkali stress, which lays the foundation for further studies on its function in apple.

Background Salt is an important factor that affects crop productivity. Plant hexokinases (HXKs) are key enzymes in the glycolytic pathway and sugar signaling transduction pathways of plants. In previous studies, we identified and confirmed the roles of GmHXK2 in salt tolerance. Results In this study, we analyzed the tissue-specific expression of GmHXK2 at different growth stages throughout the plant's life cycle. The results showed that GmHXK2 was expressed significantly in all tissues at vegetative stages, including germination and seedling. However, no expression was detected in the pods, and there was little expression in flowers during the later mature period. Arabidopsis plants overexpressing the GmHXK2 (OE) had more lateral roots. The OE seedlings also produced higher levels of auxin and ascorbic acid (AsA). Additionally, the expression levels of genes PMM, YUC4/YUC6/YUC8, and PIN/LAX1,LAX3, which are involved respectively in the synthesis of AsA and auxin, as well as polar auxin transport, were upregulated in OE plants. This upregulation occurred specifically under exogenous glucose treatment. AtHKT1, AtSOS1, and AtNHX1 were up-regulated in OE plants under salt stress, suggesting that GmHXK2 may modulate salt tolerance by maintaining ion balance within the cells and alleviating damage caused by salt stress. Additionally, we further confirmed the interaction between GmHXK2 and the protein GmPMM through yeast two-hybridization and bimolecular fluorescence complementation assays, respectively. Conclusion The expression of GmHXK2 gene in plants is organ-specific and developmental stage specific. GmHXK2 not only regulates the synthesis of AsA and the synthesis and distribution of auxin, but also promotes root elongation and induces lateral root formation, potentially enhancing soil water absorption. This study reveals the crosstalk between sugar signaling and hormone signaling in plants, where GmHXK2 acts as a glucose sensor through its interaction with GmPMM, and sheds light on the molecular mechanism by which GmHXK2 gene is involved in salt tolerance in plants.

基于含孔隙固体多孔介质理论，建立了二维层状饱和冻土地基的计算模型，研究了简谐荷载作用下层状饱和冻土地基的动力响应问题。首先，通过Helmholtz定理和Fourier积分变换，采用传递矩阵法，结合边界及层间连续条件推导获得了层状饱和冻土地基的刚度矩阵，得到了层状饱和冻土地基在频域中的动力响应解答。然后，通过快速傅里叶变换得到了层状饱和冻土中各相位移和应力的数值解。与已有文献进行对比后，通过数值算例分别分析讨论了上软下硬和上硬下软两种典型层状地基中表层土剪切模量、表层土温度、表层土孔隙率和荷载频率对动力响应的影响规律。研究结果表明：在上软下硬和上硬下软两种地基情况下，竖向位移和孔隙水所分担的应力幅值随着表层土剪切模量的增大而减小，随着表层土温度、表层土孔隙率和荷载频率的增大而增大；层状饱和冻土地基中软硬土层的排列次序对竖向位移和孔隙水所分担的应力影响显著。

基于含孔隙固体多孔介质理论，建立了二维层状饱和冻土地基的计算模型，研究了简谐荷载作用下层状饱和冻土地基的动力响应问题。首先，通过Helmholtz定理和Fourier积分变换，采用传递矩阵法，结合边界及层间连续条件推导获得了层状饱和冻土地基的刚度矩阵，得到了层状饱和冻土地基在频域中的动力响应解答。然后，通过快速傅里叶变换得到了层状饱和冻土中各相位移和应力的数值解。与已有文献进行对比后，通过数值算例分别分析讨论了上软下硬和上硬下软两种典型层状地基中表层土剪切模量、表层土温度、表层土孔隙率和荷载频率对动力响应的影响规律。研究结果表明：在上软下硬和上硬下软两种地基情况下，竖向位移和孔隙水所分担的应力幅值随着表层土剪切模量的增大而减小，随着表层土温度、表层土孔隙率和荷载频率的增大而增大；层状饱和冻土地基中软硬土层的排列次序对竖向位移和孔隙水所分担的应力影响显著。

基于含孔隙固体多孔介质理论，建立了二维层状饱和冻土地基的计算模型，研究了简谐荷载作用下层状饱和冻土地基的动力响应问题。首先，通过Helmholtz定理和Fourier积分变换，采用传递矩阵法，结合边界及层间连续条件推导获得了层状饱和冻土地基的刚度矩阵，得到了层状饱和冻土地基在频域中的动力响应解答。然后，通过快速傅里叶变换得到了层状饱和冻土中各相位移和应力的数值解。与已有文献进行对比后，通过数值算例分别分析讨论了上软下硬和上硬下软两种典型层状地基中表层土剪切模量、表层土温度、表层土孔隙率和荷载频率对动力响应的影响规律。研究结果表明：在上软下硬和上硬下软两种地基情况下，竖向位移和孔隙水所分担的应力幅值随着表层土剪切模量的增大而减小，随着表层土温度、表层土孔隙率和荷载频率的增大而增大；层状饱和冻土地基中软硬土层的排列次序对竖向位移和孔隙水所分担的应力影响显著。

With a Saturnian magnetopause average stand-off distance of about 21 planetary radii, Titan spends most of its time inside the rotating magnetosphere of its parent planet. However, when Saturn's magnetosphere is compressed due to high solar wind dynamic pressure, Titan can cross Saturn's magnetopause in the subsolar region of its orbit and therefore to interact with the shocked solar wind plasma in Saturn's magnetosheath. This situation has been observed during the T32 flyby of the Cassini spacecraft on 13 June 2007. Until a few minutes before closest approach, Titan had been located inside the Saturnian magnetosphere. During the flyby, Titan encountered a sudden change in the direction and magnitude of the ambient magnetic field. The density of the ambient plasma also increased dramatically during the pass. Thus, the moon's exosphere and ionosphere were exposed to a sudden change in the upstream plasma conditions. The resulting reconfiguration of Titan's plasma tail has been studied in real-time by using a three-dimensional, multi-species hybrid simulation model. The hybrid approximation treats the electrons of the plasma as a massless, charge-neutralizing fluid, while ion dynamics are described by a kinetic approach. In the simulations, the magnetopause crossing is modeled by a sudden change of the upstream magnetic field vector as well as a modification of the upstream plasma composition. We present real-time simulation results, illustrating how Titan's induced magnetotail is reconfigured due to magnetic reconnection. The simulations allow to determine a characteristic time scale for the erosion of the original magnetic draping pattern that commences after Titan has crossed Saturn's magnetopause. Besides, the influence of the plasma composition in the magnetosheath on the reconfiguration process is discussed in detail. The question of whether the magnetopause crossing is likely to yield a detachment of Titan's exospheric tail from the satellite is investigated as well.