This study investigates the role of arbuscular mycorrhizal fungi (AMF) in the metabolism of hexabromocyclododecane (HBCD) and its ecological effects in contaminated environments. We focused on the symbiotic relationships between Iris pseudacorus L. and AMF (Rhizophagus irregularis) under HBCD exposure. Our results show that HBCD induces oxidative damage, which hinders plant growth. However, AMF significantly enhance the plant's antioxidant defenses, reducing oxidative damage and supporting better growth of I. pseudacorus. HBCD biodegradation patterns showed beta- > gamma- > alpha-HBCD, with AMF playing a key role in stabilizing rhizosphere microbial communities, particularly promoting Proteobacteria and potential bacterial degraders like Aeromonas and Trichococcus, which contributed to HBCD removal. Additionally, AMF appear to upregulate genes such as cypD_E, GST, dehH, dehA, dehM, Em3.8.1.2, and ligB, which are involved in debromination and hydroxylation reactions. This research highlights AMF's potential to enhance the phytoremediation of HBCD, providing valuable insights for environmental remediation strategies.

Tire wear particles (TWPs) attract attention because of their harmful impact on the soil ecosystem. Nevertheless, there is limited understanding regarding how aging affects the toxicity of TWPs to soil microorganisms. Herein, a microcosm experiment was performed to compare the toxicity of pristine and UV-aged TWPs on the soil microbial community. After 28 days operation, more holes and cracks appeared on the surface of the UV-aged TWPs compared with the pristine TWPs. The diversity and community structure of soil microorganisms changed under the pristine and UV-aged TWPs exposure, with the UV-aged TWPs significantly altered nirK-type soil denitrifying bacteria. Streptomyces played an important role in connecting the nirK-type bacterial community and promoting the denitrification process under the UV-aged TWPs exposure. The soil microorganisms further promoted the membrane transport of metabolites to resist the toxic effects of UV-aged TWPs by up-regulating the ATP-binding cassette (ABC) transporters, which consumed lots of energy and led to interference in energy metabolism. Furthermore, UV-aged TWPs further stimulated the accumulation of reactive oxygen species (ROS), stimulated the soil microorganisms to secrete more extracellular polymers substances (EPS) and activated the antioxidant defense system against oxidative damage caused by UV-aged TWPs, however, the activation of SOS response in turn increased the risk of antibiotic resistance genes (ARGs) transmission.