Svalbards permafrost is thawing as a direct consequence of climate change. In the Low Arctic, vegetation has been shown to slow down and reduce the active layer thaw, yet it is unknown whether this also applies to High Arctic regions like Svalbard where vegetation is smaller, sparser, and thus likely less able to insulate the soil. Therefore, it remains unknown which components of High Arctic vegetation impact active layer thaw and at which temporal scale this insulation could be effective. Such knowledge is necessary to predict and understand future changes in active layer in a changing Arctic. In this study we used frost tubes placed in study grids located in Svalbard with known vegetation composition, to monitor the progression of active layer thaw and analyze the relationship between vegetation composition, vegetation structure and snow conditions, and active layer thaw early in summer. We found that moss thickness, shrub and forb height, and vascular vegetation cover delayed soil thaw immediately after snow melt. These insulating effects attenuated as thaw progressed, until no effect on thaw depth was present after 8 weeks. High Arctic mosses are expected to decline due to climate change, which could lead to a loss in insulating capacity, potentially accelerating early summer active layer thaw. This may have important repercussions for a wide range of ecosystem functions such as plant phenology and decomposition processes. Temperatures are rising in the Arctic, causing increased thaw of the layer of soil located above the permanently frozen ground. In Low Arctic regions vegetation cools the soil, which reduces the thawing. So far, we do not know whether the small plants growing in the High Arctic may be able to slow or reduce thaw. We measured soil thaw throughout the summer in High Arctic Svalbard in locations where vegetation composition is known. We also measured thickness of the moss layer, height of plants and snow depth. We found that moss thickness was the strongest factor in insulating the soil. Also the cover of plants, height of shrubs and forbs, and height of grass-like plants slowed soil thaw in the early summer. The insulating effects became less over time and no effects were found 8 weeks after onset of thaw. As climate change is causing changes in the Arctic vegetation, mosses and small shrubs are expected to decrease. As we found these to be the most important factors in insulating the soil, a future decrease in mosses and small shrubs may cause accelerated soil thaw at the start of summer. High Arctic vegetation slows active layer thaw in early summer after snow melt Mosses show a stronger negative relation with thaw depth than vascular vegetation Factors influencing active layer thaw change over time in early summer

Extensive, detailed information on the spatial distribution of active layer thickness (ALT) in northern Alaska and how it evolves over time could greatly aid efforts to assess the effects of climate change on the region and also help to quantify greenhouse gas emissions generated due to permafrost thaw. For this reason, we have been developing high-resolution maps of ALT throughout northern Alaska. The maps are produced by upscaling from high-resolution swaths of estimated ALT retrieved from airborne P-band synthetic aperture radar (SAR) images collected for three different years. The upscaling was accomplished by using hundreds of thousands of randomly selected samples from the SAR-derived swaths of ALT to train a machine learning regression algorithm supported by numerous spatial data layers. In order to validate the maps, thousands of randomly selected samples of SAR-derived ALT were excluded from the training in order to serve as validation pixels; error performance calculations relative to these samples yielded root-mean-square errors (RMSEs) of 7.5-9.1 cm, with bias errors of magnitude under 0.1 cm. The maps were also compared to ALT measurements collected at a number of in situ test sites; error performance relative to the site measurements yielded RMSEs of approximately 11-12 cm and bias of 2.7-6.5 cm. These data are being used to investigate regional patterns and underlying physical controls affecting permafrost degradation in the tundra biome.

Current climatic models and projections forecast further increases in air temperature in the Arctic in the future. Therefore, new knowledge concerning the variability of air and soil temperature as well as its dependence on environmental factors is urgently needed to better understand and forecast the consequences of climate warming in the Arctic region. The main aims of this study were: 1) to determine the variability of the topsoil temperature for the one-year time scale under different types of tundra vegetation in the Adventdalen Valley in the central part of Spitsbergen (Svalbard, High Arctic), and 2) to determine the impact of different types of tundra vegetation on the topsoil temperature on a microscale in this area. The obtained results indicate that wet sites covered with marsh and moss tundra vegetation exhibited a significantly higher mean annual topsoil temperature (-0.7 degrees C and -1.2 degrees C, respectively) than sites featuring bare ground (-1.5 degrees C), Arctic meadow (-2.1 degrees C), and heath tundra vegetation (-2.4 degrees C). The mean topsoil temperature during the summer season was the lowest at sites covered with Arctic meadow and heath tundra vegetation (4.9 degrees C and 5.7 degrees C, respectively), and highest at the site with bare ground (6.9 degrees C). The topsoil temperature was highly and significantly correlated with the air temperature (correlation coefficient between 0.86 and 0.92); however, the correlation was clearly higher during the autumn and summer seasons (correlation coefficient between 0.76 and 0.89; p < 0.001) in comparison with the winter season (correlation coefficient between 0.67 and 0.89; p < 0.001) and especially the spring season (correlation coefficient between 0.62 and 0.71; p < 0.001). Freeze-thaw events were substantially less frequent in the topsoil (up to 16 events) compared to the atmosphere (at least 80 events), and their number was clearly different even at the microscale (from 4 to 16 events). Local microtopography, snow cover, tundra vegetation type, soil properties, and soil moisture were responsible for variances in topsoil temperature and number of freeze-thaw events. The obtained results indicated that the topsoil temperature is significantly different, even at the microscale, and further research is needed in other Arctic terrestrial environments to better understand and forecast the consequences of climate warming in this region of the world.

Despite the importance of soil and surface waters freezing in permafrost landscapes, the behaviour of dissolved organic carbon (DOC), nutrients and metals during periodic freeze-thaw cycles (FTC) remains poorly known. The on-going climate warming is likely to increase the frequency of FTC in continental aquatic settings, which could modify the chemical composition of waters. In this study, we conducted 9 repetitive cycles of overnight freezing (similar to 20 degrees C) and 5 h thawing (4 degrees C) in the laboratory using representative 0.22 mu m-filtered waters from NE European permafrost peatland: leachates of vegetation and soil, and natural surface waters (depression, thermokarst lake and river). Only minor (10%). The leachates and the depression water were enriched in trace elements, whereas the thermokarst lake and the river demonstrated a decrease in concentration of Fe (similar to 39 and similar to 94%, respectively), Al (similar to 9 and similar to 85%), and Mn (similar to 10 and similar to 79%) during FTC. Overall, the observations demonstrated an increase in aliphatic low molecular weight organic matter (OM), and the precipitation of Fe, Al hydroxides and organo-mineral particles. Therefore, enhanced of frequency of FTC can favour the release of metals and toxicants from acidic OM-rich surface waters and maintain stable OM-metalscolloids in large lakes and rivers, thus regulating aquatic transport of DOC and metals from soils to the Arctic Ocean. (C) 2021 Elsevier Ltd. All rights reserved.

Soil organic matter (SOM) is related to vegetation, soil bacteria, and soil properties; however, not many studies link all these parameters simultaneously, particularly in tundra ecosystems vulnerable to climate change. Our aim was to describe the relationships between vegetation, bacteria, soil properties, and SOM composition in moist acidic tundra by integrating physical, chemical, and molecular methods. A total of 70 soil samples were collected at two different depths from 36 spots systematically arranged over an area of about 300 m x 50 m. Pyrolysis-gas chromatography/mass spectrometry and pyrosequencing of the 16S rRNA gene were used to identify the molecular compositions of the SOM and bacterial community, respectively. Vegetation and soil physicochemical properties were also measured. The sampling sites were grouped into three, based on their SOM compositions: Sphagnum moss-derived SOM, lipid-rich materials, and aromatic-rich materials. Our results show that SOM composition is spatially structured and linked to microtopography; however, the vegetation, soil properties, and bacterial community composition did not show overall spatial structuring. Simultaneously, soil properties and bacterial community composition were the main factors explaining SOM compositional variation, while vegetation had a residual effect. Verrucomicrobia and Acidobacteria were related to polysaccharides, and Chloroflexi was linked to aromatic compounds. These relationships were consistent across different hierarchical levels. Our results suggest that SOM composition at a local scale is closely linked with soil factors and the bacterial community. Comprehensive observation of ecosystem components is recommended to understand the in-situ function of bacteria and the fate of SOM in the moist acidic tundra. (C) 2021 Elsevier B.V. All rights reserved.

Ground surface temperature (GST) and active layer thickness (ALT) are key indicators of climate change (CC) in permafrost regions, with their relationships with climate and vegetation being crucial for the understanding of future climate change scenarios, as well as of CC feedbacks on the carbon cycle and water balance. Antarctic ice free-areas host simplified ecosystems with vegetation dominated by mosses and lichens, and an almost negligible anthropogenic impact, providing a good template of ecosystem responses to CC. At three different Antarctic Conservation Biogeographical Regions (ACBR) sites in Antarctica located between 74 degrees and 60 degrees S, we compared barren ground and moss vegetated sites to understand and quantify the effects of climate (air temperature and incoming radiation) and of vegetation on GST and ALT. Our data show that incoming radiation is the most important driver of summer GST at the southernmost site, while in the other sites air temperature is the main driver of GST. Our data indicate that there is a decoupling between ALT and summer GST, because the highest GST values correspond with the thinnest ALT. Moreover, our data confirm the importance of the buffering effect of moss vegetation on GST in Antarctica. The intensity of the effect of moss cover on GST and ALT mainly depends on the species-specific moss water retention capacity and on their structure. These results highlight that the correct assessment of the moss type and of its water retention can be of great importance in the accurate modelling of ALT variation and its feedback on CC.

Calcareous spring fens are among the rarest and most endangered wetland types worldwide. The majority of these ecosystems can be found at high latitudes, where they are affected by above average rates of climate change. Particularly winter temperatures are increasing, which results in decreased snow cover. As snow provides an insulating layer that protects ecosystems from subzero temperatures, its decrease is likely to induce stress to plants. To investigate the sensitivity of the bryophyte community - key to the functioning of calcareous spring fens - to changing climatic conditions, we studied the annual variation in ecophysiology of two dominant bryophytes: Campylium stellatum and Scorpidium scorpioides. Further, a snow removal experiment was used to simulate the effect of changing winter conditions. In both species, we observed lowest efficiency of photosystem II (Fv/Fm) in spring, indicating physiological stress, and highest chlorophyll-a, -b and carotenoid concentrations in autumn. Snowremoval exacerbated physiological stress in bryophytes. Consequently Fv/Fm, pigment concentrations and chlorophyll to carotenoids ratios declined, while chlorophyll-a to -b ratios increased. Moreover, these effects of winter climate change cascaded to the growing season. C. stellatum, a low hummock inhabitor, suffered more from snow removal (annual mean decline in Fv/Fm 7.7% and 30.0% in chlorophyll-a) than S. scorpioides, a hollow species (declines 5.4% and 14.5%, respectively). Taken together, our results indicate that spring fen bryophytes are negatively impacted by winter climate change, as a result of longer frost periods and increased numbers of freeze-thaw cycles in combination with higher light intensity and dehydration. (c) 2019 Elsevier B.V. All rights reserved.

Snow strongly affects ecosystem functioning in alpine environments with potential carry-over effects outside of snow periods. However, it is unclear whether changes in snow cover affect microbial community functioning in summer. In a field experiment, we tested whether manipulation of snow cover affected the functional capabilities of the microbial community either directly, or indirectly through concomitant changes in the vegetation. While 23 years of differential snow depth and persistence fundamentally changed the vegetation composition, the microbial community's ability to catabolize a range of carbon compounds was not altered. Instead, soil moisture content was the key driver of carbon catabolism by the microbial community.

Mosses strongly affect water and heat fluxes due their high water holding capacity and the provision of insulation. A land surface model (the coupled hydrological and biogeochemical process model, CHANGE) was used to quantitatively assess the influence of moss cover on soil temperature (T-SOIL), active layer thickness (ALT), and ecosystem carbon balance. The CHANGE model was coupled with a moss process module, enabling the explicit representation of heat, water, and carbon exchange in the atmosphere-vegetation-moss-soil system. The model was applied to a tundra site in northeastern Siberia over the period of 1980-2013. The results were validated with in situ observations and indicated a high level of insulation by the moss, resulting in warmer winter and cooler summer T-SOIL and smaller ALT. The sensitivities of T-SOIL and ALT to moss coverage and thickness were examined using model experiments. An increase in moss thickness lowered the summer T-SOIL by 0.9-2.1 degrees C and reduced ALT by 9-20cm compared with a moss-free experiment. The moss-induced cooler T-SOIL in the root zone could limit the productivity of vegetation by reducing water availability to plant roots due to the presence of ice. This limitation increased with increasing moss layer thickness and coverage. The productivity of the moss itself increased with thickness, partially offsetting the reduction in vegetation productivity. Our modeling study suggests that the moss layer has a significant impact on T-SOIL, ALT, and carbon balance in the Arctic tundra and may play an important role in future Arctic warming.

In the boreal and subarctic zone, the moss and peat interactions with rainwater and snowmelt water in shallow surface ponds control the delivery of dissolved organic matter (DOM) and metal to the rivers and further to the Arctic Ocean. The transformation of peat and moss leachate by common aquatic microorganisms and the effect of temperature on DOM mineralization by heterotrophs remain poorly known that does not allow predicting the response of boreal aquatic system to ongoing climate change. We used experimental approach to quantify the impact of boreal aquatic bacteria P. reactans, and two culturable bacteria extracted from a thaw lake of the permafrost zone (Bolshezemelskaya tundra, NE Europe): Iodobacter sp. and cyanobacterial associate dominated by order Chroococcales (Synechococcus sp). The interaction of these bacterial cultures with nutrient-free peat and moss leachate was performed in order to (1) quantify the impact of temperature (4, 25 and 45 A degrees C) on peat leachate processing by heterotrophs; (2) compare the effect of heterotrophic bacteria and cyanobacterial associate on moss and peat leachate chemical composition, and (3) quantify the DOC and metal concentration change during cyanobacterial growth on leachate from frozen and thawed peat horizon and moss biomass. The efficiency of peat DOM processing by two heterotrophs was not modified by temperature rise from 4 to 45 A degrees C. The DOC concentration decreased by a factor of 1.6 during 3 days of moss leachate reaction with Iodobacters sp. or cyanobacterial associate at 25 A degrees C. The SUVA(245) increased twofold suggesting an uptake of non-aromatic DOM by both microorganisms. The growth of cyanobacteria was absent on peat leachate but highly pronounced on moss leachate. This growth produced tenfold decrease in P concentration, a factor of 1.5-2.0 decrease in DOC, a factor of 4 and 100 decrease in Fe and Mn concentration, respectively. Adsorption of organic and organo-mineral colloids on bacterial cell surface was more important factor of element removal from organic leachates compared to intracellular assimilation and/or Fe oxyhydroxide precipitation. Overall, we demonstrate highly conservative behavior of peat leachate compared to moss leachate in the presence of culturable aquatic bacteria, a lack of any impact of heterotrophs on peat leachate and their weak impact on moss leachate. A very weak temperature impact on DOM processing by heterotrophs and lack of difference in the biodegradability of DOM from thawed and frozen peat horizons contradict the current paradigm that the warming of frozen OM and its leaching to inland waters will greatly affect microbial production and C cycle. Strong decrease in concentration of P, Fe and Mn in the moss leachate in the presence of cyanobacterial associate has straightforward application for understanding the development of thermokarst lakes and suggests that, in addition to P, Fe and Mn may become limiting micronutrients for phytoplankton bloom in thermokarst lakes.