Several slug species are serious pests of agriculture and are difficult to control. One popular control method is the nematode Phasmarhabditis hermaphrodita, which has been used in slug control for > 25 years. However, there are reports of it failing to reduce slug numbers and damage in the field for unknown reasons. This may be due to lack of knowledge about how P. hermaphrodita performs when applied to different soils. We therefore assessed the survival, movement and pathogenicity of P. hermaphrodita infective juveniles (IJs) when added to six different soils (compost with and without peat, clay loam, loam, sandy loam and sandy soil). The soils were either frozen or autoclaved before use to eradicate resident nematodes prior to the experiment. P. hermaphrodita survived best in autoclaved compost without peat and in experiments with frozen soils, compost with and without peat was best. Survival of P. hermaphrodita was similar in other soils. Interestingly, in peat-free compost P. hermaphrodita reproduced prolifically, which may affect the long-term success of the nematode in the field as other life stages, apart from the IJ stage, cannot infect slugs. In infection experiments we found P. hermaphrodita added to compost with peat killed slugs faster than nematodes added to a sandy clay loam or sandy soil. In movement experiments, the nematodes remained within 3 cm of the application point in each soil. In summary, soil type severely affects P. hermaphrodita survival, and the ability to kill slugs; therefore it should be assessed by farmers and gardeners before use.

The European hare ( Lepus europaeus), ), commonly known as the gray hare, belongs to the Leporidae family. Widely distributed throughout Poland, it exhibits higher population density in the eastern regions. However, Europe has witnessed a significant decline in hare populations over the past 50 years, a trend also observed in most Polish areas. Contributing factors include intensified agriculture, habitat loss, urbanization, pollution (water, air, and soil), and climate change, all impacting population dynamics, including reproduction. Increased predation by foxes, raccoons, and birds of prey, along with poaching and hunting-related damage, further affect hare populations. Infectious and parasitic diseases play a crucial role in hare health, often leading to mortality. These animals constantly interact with various pathogens and vectors due to their natural environment. The objective of this study was to assess the health of the European hare population in the Lublin Upland based on macroscopic findings during autopsies. The research involved 85 hares from two hunting districts in the western part of the Lublin Upland. These animals were necropsied, and material was collected for laboratory tests. Macroscopic examinations revealed the presence of several diseases, including brucellosis (6 cases), rodentiosis (11 cases), tularemia (3 cases), echinococcosis (5 cases), and cysticercosis (11 cases). Most of these diseases are classified as dangerous zoonotic infections. The detection of multiple diseases underscores the gray hare's role as a reservoir in the natural environment. These confirmed diseases pose significant risks, often proving fatal for both hares and their predators. As a game species, European hares also pose a threat to hunters who handle infected animals. Moreover, meat from sick hares jeopardizes consumer health and safety. This research holds substantial importance for veterinary medicine and public health, aiding in early diagnosis and effective disease prevention strategies.