Soil salinization threatens sustainable agriculture, necessitating innovative restoration strategies. Suaeda salsa (L.) Pall., a halophyte with exceptional salt tolerance and vivid pigmentation, serves as an ideal model for salinity adaptation. This study integrates physiological and transcriptomic analyses to reveal how high salinity (400 mmolL(-)1 NaCl) upregulates 4,5-DOPA dioxygenase after 30 days of salt stress, promoting betacyanin accumulation to mitigate oxidative damage. Compared to the control, betacyanin content in the 200 mmolL(-)1 and 400 mmolL(-)1 NaCl groups increased to 1.975-fold and 3.675-fold, respectively, while chlorophyll a content decreased by 45.78% and 69.88%, chlorophyll b by 11.45% and 28.24%, and total chlorophyll by 30.28% and 53.06%. This trade-off in pigment allocation highlights the plant's adaptive strategy under salinity stress. The photosynthetic characteristics of S. salsa confirm that its photoprotective mechanisms are significantly enhanced under 400 mmolL(-)1 NaCl. At the molecular level, betacyanin biosynthesis alleviates oxidative stress, while transcriptional regulation of photosystem I (PSI) and photosystem II (PSII) genes-such as PsbY, PsaO, PsbM, and PsbW-partially restores photosynthetic activity. Stabilization of the electron transport chain by upregulated genes like PetA and PetH further enhances photosynthetic resilience. These findings highlight the synergy between betacyanin production and photosynthetic regulation in enhancing salinity resilience, providing insights for soil restoration and salt-tolerant crop breeding.

A pot-controlled watering approach was employed to reveal the effect of soil water stress on photosynthetic physiology of Paspalum notatum Flugge under special climatic conditions in arid-hot valley region. Four treatments were set up: control (CK), low stress (LS), moderate stress (MS), and high stress (HS). Physiological measurements were taken to assess indices such as absolute plant height, canopy area, leaf area, leaf water content, and leaf water potential. Additionally, photosynthetic parameters were measured, including net photosynthetic rate, intercellular CO2 concentration, stomatal conductance and chlorophyll fluorescence. The results indicate that under water stress, as the duration of stress increases, the growth of Paspalum notatum Flugge was inhibited, the water available in the body of Paspalum notatum Flugge gradually decreased. Photosynthesis was inhibited and PS II reaction center was disrupted to some extent. To improve water retention, Paspalum notatum Flugge initiated self-protective mechanisms, diminishing leaf water potential and enhancing ability to absorb water from the soil. In the meantime, Paspalum notatum Flugge adjusted to adversity by reducing the stomatal aperture to inhibit water loss, lowering Tr, and increasing WUE. The experiment showed that after rehydration, damaged photosynthetic apparatus of Paspalum notatum Flugge retained a certain self-recovery capability. This phenomenon suggests the reversible deactivation of the photosynthetic apparatus in response to water stress.

To address water scarcity and soil damage in the Hexi Oasis irrigation area of China, a study was conducted on regulating water and nitrogen levels for soybean growth under film drip irrigation over two growing seasons (2020 and 2021). Two irrigation levels were tested: mild deficit (W1, 60-70% of field water capacity, FC) and full irrigation (W2, 70-80% of FC), along with three nitrogen levels: low (N1, 60 kgha-1), medium (N2, 120 kgha-1), and high (N3, 180 kgha-1). The control treatment was no nitrogen with full irrigation (W2N0), totaling seven treatments. Results showed that during both growing seasons, soybean plant height reached its peak at the tympanic ripening stage, while the leaf area index (LAI), net photosynthesis rate (Pn), and transpiration rate (Tr) decreased at the tympanic ripening stage. The highest values for the plant height, LAI, Pn, Tr, yield, and the cost-benefit ratio were observed under the W2N2 treatment, significantly outperforming the W2N0 in all aspects (p < 0.05). Over the two-year period, the plant height and LAI were notably higher by 22.86% and 7.09%, respectively, in the W2N2 treatment compared to the W1N1. Full irrigation under N1 and N2 conditions resulted in an enhanced soybean Pn and Tr. However, under N3 conditions, a deficit-tuned irrigation treatment led to a 15.71% increase in the Pn and a 13.34% increase in the Tr on a two-year average. The W2N2 treatment had the highest yield, with a significant 4.93% increase over the W1N3 treatment on a two-year average. The highest rate of change in yield was observed in W1. The two-year cost-benefit ratio and unilateral water benefit reached their peak values in W2N2 and W1N2, respectively. Water use efficiency (WUE) was lower in N1 but significantly increased by 21.83% on a two-year average in W1N3 compared to W1N2. Additionally, W1 had a 14.21% higher WUE than W2 over two years. N3 had the lowest partial factor productivity of nitrogen, which increased by 17.78% on a two-year average in W2N1 compared to W1N1. All nine indicators related to yield formation and water-nitrogen use efficiency showed a positive correlation (p < 0.05) in this study. The highest composite scores were achieved with the W2N2 treatment in both years using the entropy weight and TOPSIS method. Overall, the W2N2 treatment provides a water and nitrogen combination that enhances soybean water and fertilizer efficiency, making it a promising option for high-yield soybean cultivation with water and nitrogen conservation in the Hexi Oasis irrigation area of China. This study offers valuable insights for achieving efficient soybean production while saving water and reducing nitrogen use.

The increasing use of herbicides in intelligent agricultural production is driven by the time-consuming nature of manual weeding, as well as its ephemeral effectiveness. However, herbicides like butachlor degrade slowly and can be washed away by rainwater, ultimately flowing into the farm ponds and posing risks to aquatic plants. To identify and recommend superior restoration strategies that effectively address the challenges posed by butachlor, we investigated the impacts of butachlor on the growth and physiology of four common aquatic plants (i.e., Hydrilla verticillata, Ceratophyllum demersum, Potamogeton maackianus, and Myriophyllum aquaticum) and their potential role in mitigating environmental damage by reducing residual herbicide levels. Our findings indicated that M. aquaticum was tolerant to butachlor, exhibiting higher growth rates than other species when exposed to various butachlor concentrations. However, the concentration of butachlor negatively impacted the growth of H. verticillata, C. demersum, and P. maackianus, with higher concentrations leading to more significant inhibitory effects. After a 15-day experimental period, aquatic plants reduced the butachlor residuals in culture mediums across concentrations of 0.5 mg/L, 1 mg/L, and 2 mg/L compared to non-plant controls. Our findings classified P. maackianus as butachlor-sensitive and M. aquaticum as butachlor-tolerant species. This investigation represents novel research aimed at elucidating the contrasting effects of different concentrations of butachlor on four common aquatic species in the agricultural multi-pond system.