This study aimed to evaluate the synergistic effects of zinc sulfate and Pseudomonas spp. in terms of mitigating drought stress in maize (Zea mays L.) by analyzing physiological, biochemical, and morphological responses under field conditions. A two-year (2018-2019) field experiment investigated two irrigation levels (optimal and moderate stress) and twelve treatment combinations of zinc sulfate application methods (without fertilizer, soil, foliar, and seed priming) with zinc-solubilizing bacteria (no bacteria, Pseudomonas fluorescens, and Pseudomonas aeruginosa). Drought stress significantly reduced chlorophyll content, increased oxidative damage, and impaired membrane stability, leading to a 42.4% increase in electrolyte leakage and a 10.9% reduction in leaf area index. However, the combined application of zinc sulfate and P. fluorescens, and P. aeruginosa mitigated these effects, with seed priming showing the most significant improvements. Specifically, seed priming with zinc sulfate and P. fluorescens increased catalase activity by 76% under non-stress conditions and 24% under drought stress. Principal component analysis revealed that treatments combining zinc sulfate and P. fluorescens, and P. aeruginosa were strongly associated with improved chlorophyll content, carotenoid content, and grain yield while also enhancing osmotic adjustment and antioxidant enzyme activity. These findings highlight the potential of the use of zinc sulfate and P. fluorescens as well as P. aeruginosa as sustainable strategies for enhancing maize drought tolerance, mainly through seed priming and soil application methods.
The literature shows that a circular economy can benefit some sectors such as the construction industry. This sector demands huge amounts of raw materials and produces waste when buildings and structures are demolished. This paper explores the possibility of manufacturing at industrial scale paving blocks using different types of construction and demolition wastes as aggregates, without modifying the commonly used industrial conditions. A total of four different recycled aggregates were used in this research. Both natural and recycled aggregates have been characterized. The dosages were optimized (three different formulations). Prefabricated tests have been carried out on the products manufactured in industrial plants and the evolution of mechanical properties over time has been analysed. The results obtained were analysed statistically by applying the principal component analysis (PCA) method. To ensure the security of the elements manufactured, the ionic leaching of the materials used as recycled aggregate and of the elements produced has been tested. The main implications of this research on the construction industry show that the majority of recycled aggregates used could replace 25% of the natural aggregate in manufactured precast concrete, that the properties of the aggregates should be taken into account in the different standards and that all paving blocks manufactured in this study can be considered environmentally safe (no risk of leaching) according to the Netherland Soil Quality Decree. Therefore, it is evident that it is possible to manufacture on an industrial scale paving blocks with mixed recycled aggregates, concrete and ceramic in nature, both with the fine and coarse fractions that meet the requirements of its reference standard UNE-EN 1338 and the Netherland Soil Quality Decree that evaluates environmental risks due to leaching.