As the basic units of soil structure, soil aggregate is essential for maintaining soil stability. Intensified freeze-thaw cycles have deeply affected the size distribution and stability of aggregate under global warming. To date, it is still lacking about the effects of freeze-thaw cycles on aggregate in the permafrost regions of the Qinghai-Tibetan Plateau (QTP). Therefore, we investigated the effects of diurnal and seasonal freeze-thaw processes on soil aggregate. Our results showed that the durations of thawing and freezing periods in the 0-10 cm layer were longer than in the 10-20 cm layer, while the opposite results were observed during completely thawed and frozen periods. Freeze-thaw strength was greater in the 0-10 cm layer than that in the 10-20 cm layer. The diurnal freeze-thaw cycles have no significant effect on the size distribution and stability of aggregate. However, 0.25 mm) and reduced aggregate stability. Our study has scientific guidance for evaluating the effects of freeze-thaw cycles on soil steucture and provides a theoretical basis for further exploration on soil and water conservation in the permafrost regions of the QTP.

Air temperatures are rising and the winter snowpack is getting thinner in many high-latitude and high-elevation ecosystems around the globe. Past studies show that soil warming accelerates microbial metabolism and stimulates soil carbon (C) and nitrogen (N) cycling. Conversely, winter snow removal to simulate loss of snow cover leads to increased soil freezing and reductions in soil microbial biomass, exoenzyme activity, and N cycling. The Climate Change Across Seasons Experiment (CCASE), located at Hubbard Brook Experimental Forest, NH (USA) is designed to evaluate the combined effects of growing season soil warming and an increased frequency of winter soil freeze-thaw cycles on a northern forest ecosystem. Soils were collected from CCASE over two years (2014 and 2015) and extractable C and N pool sizes, as well as microbial biomass, exoenzymes, and potential net N mineralization and microbial respiration were measured. Soil warming alone did not stimulate microbial activity at any sampling time. Extractable amino acid N and organic C, proteolytic and acid phosphatase activity, and microbial respiration were reduced by the combination of warming in the growing season and winter soil freeze-thaw cycles during the period following snowmelt through tree leaf out in spring. The declines in microbial activity also coincided with an 85% decline in microbial biomass N at that time. Growing season warming and winter soil freeze-thaw cycles also resulted in a two-fold reduction in phenol oxidase activity and a 20% reduction in peroxidase activity and these declines persisted throughout the snow-free time of the year. The results from this study suggest that positive feedbacks between warming and rates of soil C and N cycling over the next 100 years will be partially mitigated by an increased frequency of winter soil freeze-thaw cycles, which decrease microbial biomass and rates of soil microbial activity.