Climate warming has impacted the sustainability of freshwater supply in the global water tower unit (WTU) zone. The rainfall infiltration process, a key component of WTUs supply, is affected by freeze-thaw cycles, yet it remains uncertain whether it has undergone corresponding changes. We propose a temperature-mediated infiltration model considering changes in soil water holding, water potential, and hydraulic conductivity due to varying degrees of freezing under negative temperature. Using this model, we calculate the infiltration of 78 WTUs globally from 1980 to 2023. Our results indicate that global WTUs have a multi-year average infiltration of 26 similar to 2359 mm/year. Notably, WTUs in the key latitudinal zone (24 degrees S-42 degrees N) contribute 54 % of the total infiltration volume, showing expanding differences in infiltration characteristics compared to other regions. While rainfall primarily influences infiltration and infiltration capacity, soil temperature and initial soil water content also significantly impact these characteristics. Enhanced infiltration capacity promotes vegetation growth, though the relationship is not linear. Variations in infiltration characteristics threaten the water resource buffering and the stability of downstream living ecological water supply of WTUs. This study provides crucial references for the integrated management of water resources and ecological conservation amid changing infiltration characteristics.