Phenotyping yam (Dioscorea spp.) germplasm for resistance to parasitic nematodes is hampered by the lack of an efficient screening method. In this study, we developed a new method using rooted yam vine cuttings and yam plantlets generated from semi-autotrophic hydroponics (SAHs) propagation for phenotyping yam genotypes for nematode resistance. The method was evaluated using 26 genotypes of D. rotundata for their reaction to Scutellonema bradys and four root-knot nematode species, Meloidogyne arenaria, M. enterolobii, M. incognita, and M. javanica. Yam plantlets established in nursery bags filled with steam-sterilized soil were used for screening against single nematode species. Plants were inoculated four weeks after planting and assessed for nematode damage eight weeks later. A severity rating scale was used to classify genotypes as resistant, tolerant, or susceptible determine based on the nematode feeding damage on tubers and the rate of nematode multiplication in the roots of inoculated plants. The results demonstrated putative resistance and tolerance against S. bradys in 58% of the genotypes and 88%, 65%, 65%, and 58% against M. arenaria, M. javanica, M. incognita, and M. enterolobii, respectively. The method is rapid, flexible, and seasonally independent, permitting year-round screening under controlled conditions. This method increases the throughput and speed of phenotyping and improves the selection process.

The significance of quantifying the interaction of other non-dust particles with solar radiation cannot be overemphasized. This paper presents the radiative forcing aerosol effects of some non-dust particles over four different climatic zones of West Africa. Aerosol radiative effects on solar radiation require accurate analysis of optical and radiative properties. Radiative forcing was determined by anthropogenic, dust, marine, and non-dust aerosols governed by their size distribution and concentration. A consistent increase in daily AOD values was observed with decreasing angstrom exponent. Results showed that high negative forcing was experienced in the Savanna and Guinea zones which can be attributed to the addition of black carbon and organic matter aerosols to the heavily deposited dust in the atmosphere. Non-dust and anthropogenic aerosols were found to be major contributors to the high atmospheric absorption. The result also shows that the observed variations in the aerosol properties indicate an increase in the surface cooling in the early days of February. Therefore, a larger quantity of anthropogenic and non-dust aerosols, apart from the predominant dust, could cause and boost the radiative forcing of aerosols over West Africa.

The strategic location of the AERONET site in Ilorin, Nigeria, makes it possible to obtain information on several aerosol types and their radiative effects. The strong reversal of wind direction occasioned by the movement of the ITCZ during the West Africa Monsoon (WAM) plays a major role in the variability of aerosol nature at this site, which is confirmed by aerosol optical depth (AOD) (675 nm) and angstrom ngstrom exponent (AE) (440-870 nm) values with 1st and 99th percentile values of 0.08 and 2.16, and 0.11 and 1.47, respectively. The direct radiative forcing (DRF) and radiative forcing efficiency (RFE) of aerosol, as retrieved from the AERONET sun-photometer measurements, are estimated using radiative transfer calculations for the periods of 2005-2009 and 2011-2015. The DRF and RFE of the dominant aerosol classes-desert dust (DD), biomass burning (BB), urban (UB) and gas flaring (GF)-have been estimated. The median (+/- standard deviation) values of the DRF at the top of the atmosphere (TOA) for the DD, BB, UB and GF aerosol classes are -27.5 +/- 13.2 Wm(-2), -27.1 +/- 8.3 Wm(-2), -11.5 +/- 13.2 Wm(-2) and -9.6 +/- 8.0 Wm(-2), respectively, while those of the RFE are -26.2 +/- 4.1 Wm(-2) delta(-1), -35.2 +/- 4.6 Wm(-2) delta(-1), -31.0 8.4 Wm(-2) delta(-1) and -37.0 +/- 10.3 Wm(-2) delta(-1), respectively. Arguably due to its high SSA and assymetric values, the DD aerosol class shows the largest DRF but the smallest RFE. Its smallest AOD notwithstanding, the GF class can cause greater perturbation of the earth-atmosphere system in the sub-region both directly and indirectly, possibly due to the presence of black carbon and other co-emitted aerosol and the ageing of the GF aerosols. This study presents the first estimate of DRF for aerosols of gas flaring origin and shows that its radiative potential can be similar in magnitude to that of biomass burning and urban aerosol in West Africa.

This study assesses the direct and indirect effects of natural and anthropogenic aerosols (e.g., black carbon and sulfate) over West and Central Africa during the West African monsoon (WAM) period (June-July-August). We investigate the impacts of aerosols on the amount of cloudiness, the influences on the precipitation efficiency of clouds, and the associated radiative forcing (direct and indirect). Our study includes the implementation of three new formulations of auto-conversion parameterization [namely, the Beheng (BH), Tripoli and Cotton (TC) and Liu and Daum (R6) schemes] in RegCM4.4.1, besides the default model's auto-conversion scheme (Kessler). Among the new schemes, BH reduces the precipitation wet bias by more than 50% over West Africa and achieves a bias reduction of around 25% over Central Africa. Results from detailed sensitivity experiments suggest a significant path forward in terms of addressing the long-standing issue of the characteristic wet bias in RegCM. In terms of aerosol-induced radiative forcing, the impact of the various schemes is found to vary considerably (ranging from -5 to -25 W m(-2)).