Legumes are a vital component of agriculture, providing essential nutrients to both humans and soil through their ability to fix atmospheric nitrogen. However, the production of legume crops is often hindered by various biotic and abiotic stresses, limiting their yield and nutritional quality of crops by damaging plant tissues, which can result in lower protein content, reduced levels of essential vitamins and minerals, and compromised seed quality. This review discusses the recent advancements in technologies that are revolutionizing the field of legume crop improvement. Genetic engineering has played a pivotal role enhancing legume productivity. Through the introduction of genes encoding for enzymes involved in nitrogen fixation, leading to higher yields and reducing the reliance on synthetic fertilizers. Additionally, the incorporation of genes conferring disease and pest resistance has significantly reduced the need for chemical pesticides, making legume cultivation more sustainable and environmentally friendly. Genome editing technologies, such as CRISPR-Cas9, have opened new avenues for precision breeding in legumes. Marker-assisted selection and genomic selection are other powerful tools that have accelerated the breeding process. These techniques have significantly reduced time and resources required to develop new legume varieties. Finally, advancement technologies for legume crop improvement are aid and enhancing the sustainability, productivity, and nutritional quality of legume crops.

This study was carried out to evaluate the interaction between terrestrial food crop plants and microplastics (MPs) with a focus on understanding their uptake, effects on growth, physiological, biochemical, and yield characteristics of two different cultivars of Solanum tuberosum L. i.e., Variety-1, Astrix (AL-4) and Variety-2, Harmes (WA-4). Polyethylene (PE), polystyrene (PS), and polypropylene (PP) spheres of size 5 mu m were applied to the soil at concentrations of 0 %, 1 %, and 5 %. Morphological parameters, including seed germination rate, shoot and root lengths, leaf area, and fresh and dry biomass of plants, got reduced significantly with the increase in MP concentration. PS MPs caused the most negative impact, particularly at 5 %, leading to the greatest decline in growth and Na, Mg, Zn, Cu, Ni, and Mn nutrient content. The highest DPPH scavenging activity was observed in the 5 % PS MPs treatment with approximately a 45.34 % increase from the control, indicating its potential to enhance antioxidant activity in response to stress caused by PS MPs. Both reducing and non-reducing sugar contents and total proteins were also decreased significantly. Vitamin C content exhibited a significant increase in response to MPs, with the highest levels recorded under 5 % PS MPs treatments. This suggests an adaptive antioxidant response to mitigate oxidative damage induced by MPs. SEM analysis revealed tissue infiltration of MP particles in shoots, leaves, and tubers of both varieties. Among MPs, PS had the most detrimental effects, followed by PP and PE, with higher concentrations increasing the negative impact.

Contact Lens (CLs) are often disposed of via toilet or sinks, ending up in the wastewater treatment plants(WWTPs). Millions of CLs enter WWTPs worldwide each year in macro and micro sizes. Despite WWTPs'ability to remove solids, CLs can persist and potentially contaminate watercourses and soils. This study evaluates whether different CLs degrade in WWTP aeration tanks. Six daily CLs (Nelfilcon A,Delefilcon A, Nesofilcon A, Stenfilcon A, Narafilcon A, Somofilcon A) and four monthly CLs (Lotrafilcon B,Comfilcon A, Senofilcon A, and Samfilcon A) were immersed in aeration tanks for twelve weeks. Theirphysical and chemical properties, including water content (WC), refractive index (RI), chemical prop-erties (Fourier Transform Infrared Spectroscopy), and mechanical properties were assessed. Results show that all CLs maintained their physical appearance after 12 weeks. Neither Nelfilcon A norNarafilcon A exhibited significant changes in WC and RI, (p>0.05, Tukey test), while other daily lensesshowed variations in at least one parameter. Among monthly CLs, only Senofilcon A showed significant differences in both WC (p0.05 Tukey test). However, Somofilcon A displayed significant changes in stress at break (p<0.0001,Tukey test), and Elongation at Break (p<0.05, Tukey test). No changes were found in the chemicalstructure of any CLs suggesting that twelve weeks in WWTP aeration tanks is insufficient for CLsdegradation. Thesefindings highlight CLs as a potential emerging pollutant, emphasizing their persis-tence in sludge or migration into watercourses and soils (c) 2025 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. Thisis an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Soil organisms are key to plant growth and ecosystem functions. Earthworms (EWs) enhance soil and indirectly affect plant growth, while their cutaneous excreta (CEx) contain bioactive compounds capable of eliciting plant responses. However, their role in plant immunity is still not well understood. We hypothesized that EWs and their CEx enhance plant defense against foliar pathogens by activating induced resistance. To test this, we evaluated the effect of Eisenia fetida and their CEx on Solanum lycopersicum (tomato), focusing on growth, physiology, and defense response against Botrytis cinerea. Plants were exposed to EWs, CEx, or water (control), followed by B. cinerea infection after two weeks. Gene expression of defense markers was assessed at 24 and 48 h post-inoculation (hpi), while physiological parameters and disease severity were evaluated at 72 hpi. EWs increased shoot biomass compared to CEx, while both treatments reduced root dry weight, suggesting a possible shift in resource allocation. CEx significantly reduced B. cinerea-induced leaf damage and showed a trend for flavonoid accumulation, a known marker of induced resistance. Both treatments, EWs and CEx, activated the jasmonic acid (JA) signaling pathway, with CEx specifically upregulating genes involved in fungal pathogen defense, sustaining their expression over time. The present study offers, for the first time, clear evidence that EW derived CEx can induce resistance by stimulating plant defense responses. Further biochemical, transcriptomic, and metabolomic analyses are needed to confirm indirect results, along with field validation. Nonetheless, the findings underscore the crucial role of soil biodiversity in enhancing crop resilience.

CONTEXT: Policy issues in most nations include adapting primary agricultural production to reduce greenhouse gas (GHG) emissions. Commitments have been established through multi-lateral agreements targeting GHG emission reductions to abate climate change impacts. In response to policy initiatives targeted at industries such as agriculture, producers are adopting innovative production methods and technologies to provide environmental services and mitigate emissions. GHG emissions arising from livestock production contribute to a damaging narrative surrounding agriculture, particularly beef production. OBJECTIVE: The purpose of this study is three-fold, quantifying (a) net emissions,2 (b) changes in practice, and (c) economic outcomes attributed to the forage production facet of cow-calf production. METHODS: The Saskatchewan Forage Production Survey was developed to gather forage management practices data, placing emphasis on land use and land management changes. Canada's whole-farm assessment model, Holos, was applied as a carbon accounting framework to derive the net emissions of the forage production cycle. RESULTS AND CONCLUSIONS: Results indicate carbon sequestration increased between the periods of 1991-94 and 2016-19. Gross emissions decreased to a larger degree and net emission results for the forage production facet of the Saskatchewan cow calf sector are -0.123 Mg CO2e/ha/yr in 2016-19. SIGNIFICANCE: Recommendations include the renewal of forage rejuvenation funding programs that may improve forage yields and carbon sequestration potential. Further, the expansion of term conservation easement programs to include non-native forage lands is recommended to incentivize the retention of forage land.

Periphyton-based biofertilizer have a high potential for soil remediation, particularly for controlling soil salinization. This global environmental problem leads to low soil utilization and insufficient crop yields. Efficient and sustainable methods of managing saline soils are needed to reduce salinization and improve soil fertility and crop quality. Traditional methods such as physical mulching and chemical amendments, while improving soil conditions, exhibit limited effectiveness and may damage soil structure. This study aims to evaluate the feasibility of algae-based fertilizers in remediating saline-alkali soils and improving crop performance. The review delves into the and application prospects of algae-based fertilizers, highlighting their potential from both sustainable development and economic perspectives. It further advocates integrating other emerging technologies with the production and application of algae-based fertilizers to address the increasingly severe challenges posed by degraded soil resources and environmental instability. The review found that algal fertilizers are more environmentally friendly than traditional chemical fertilizers but are not inferior in function. This approach offers more efficient and sustainable solutions for managing saline-alkaline soils and effectively achieves sus-tainable agricultural production. Furthermore, it is necessary to conduct experimental research and monitoring evaluations of algal fertilizers to formulate scientific and rational fertilization plans to meet the increasingly serious challenges facing soil resources and unstable environments. The findings of this study will provide theoretical and technical support for using algae biofertilizers for soil remediation, improving crop quality and sequestering carbon.

The hilly and mountainous regions of China are characterized by unique features such as small plots of land, steep slopes, fragmented fields, and high soil viscosity, which result in a decline in the efficiency of conventional agricultural machinery, or even render its use impractical. To address this issue, this study developed a micro universal chassis adapted to hilly terrains. First, a four-wheel-drive multifunctional electric micro chassis was designed, considering the terrain characteristics of hilly regions and the agronomic requirements of maizesoybean strip intercropping. Second, the kinematics of the chassis were modeled and analyzed to determine optimal posture control strategies, and a fuzzy RBF neural network-based PID control algorithm was designed to enable dynamic adjustment of the chassis. Then, extensive testing was conducted on the prototype chassis, including straight-line driving tests, steering tests, climbing tests, and passability tests, which demonstrated its excellent operational performance. The straight-line driving tests showed an average lateral deviation of 30 mm and a maximum deviation of 60 mm, while the in-situ steering tests recorded a deviation of 20 mm. Finally, the prototype was applied to field weeding operations, where results indicated that its performance, including travel speed, weeding efficiency, and seedling damage rate, significantly outperformed existing traditional models. The findings suggest that the designed multifunctional micro universal chassis is highly effective for use in hilly and mountainous regions, with superior performance particularly under intercropping systems.

It has not been known how immune responses in soil invertebrates occur against microplastics (MPs). This study aims to investigate the effects of MPs on endocytosis, including phagocytosis and pinocytosis, of immune cells of soil invertebrates in the soil ecosystem in the process of bacterial infection. We employed polystyrene micro- plastics (similar to 1 mu m PS MPs) to treat earthworm Eisenia andrei during the infection of Escherichia coli for in vitro (1, 5, 10, and 50 mg/L) and in vivo (1, 10, and 1000 mg/kg dry soil) assays. The results of in vitro migration assay revealed that MPs caused inhibitory effects on the phagocytosis, pinocytosis and oxidative stress in coelomocytes. Soil bioassay also confirmed that endocytosis of coelomocytes and mitochondrial damages in the intestinal epithelium were significantly altered in the polluted soil with MPs. Thus, MPs induced adverse effects to inhibit bacterial endocytosis, which may disturb the immune system of soil invertebrates. This study is the first report on the inhibition of phagocytosis in the soil invertebrates by MPs. These findings contribute to understanding the response of soil invertebrates, which play important roles in the soil food web with cellular level towards microplastic pollution in soil.

The unique optical properties of microplastic particles have a significant impact on atmospheric radiative forcing. Based on the generalized multi-particle Mie theory, this paper presents a comparative study of the extinction properties and absorption properties of single-component and mixed aerosol clusters composed of microplastics, dust, and black carbon in different structural forms and particle sizes. The results show that the structure, particle size, mixing arrangement, and orientation of aerosol particles containing microplastics will directly affect their optical properties. As the incident wavelength increases, significant differences are observed in the extinction and absorption cross-sections of microplastic and dust particle chains with different structures, although they exhibit similar trends. However, black carbon particle chains show a distinct variation pattern. In the mixed particle chains with different particle sizes, as the incident wavelength increases, the extinction and absorption cross-sections are significantly larger than those of the particle chains with the same particle size, indicating that the particle size has a remarkable influence on their optical properties. The different mixing forms and orientations of aerosol clusters also significantly affect their extinction and absorption cross-sections. These findings provide a new theoretical perspective for environmental optics and remote sensing monitoring of aerosols.

The wheat powdery mildew (WPM) is one of the most severe crop diseases worldwide, affecting wheat growth and causing yield losses. The WPM was a bottom-up disease that caused the loss of cell integrity, leaf wilting, and canopy structure damage with these symptoms altering the crop's functional traits (CFT) and canopy spectra. The unmanned aerial vehicle (UAV)-based hyperspectral analysis became a mainstream method for WPM detection. However, the CFT changes experienced by infected wheats, the relationship between CFT and canopy spectra, and their role in WPM detection remained unclear, which might blur the understanding for the WPM infection. Therefore, this study aimed to propose a new method that considered the role of CFT for detecting WPM and estimating disease severity. The UAV hyperspectral data used in this study were collected from the Plant Protection Institute's research demonstration base, Xinxiang city, China, covering a broad range of WPM severity (0-85 %) from 2022 to 2024. The potential of eight CFT [leaf structure parameter (N), leaf area index (LAI), chlorophyll a + b content (Cab), carotenoids (Car), Car/Cab, anthocyanins (Ant), canopy chlorophyll content (CCC) and average leaf angle (Deg)] obtained from a hybrid method combining a radiative transfer model and random forest (RF) and fifty-five narrow-band hyperspectral indices (NHI) was explored in WPM detection. Results indicated that N, Cab, Ant, Car, LAI, and CCC showed a decreasing trend with increasing disease severity, while Deg and Car/Cab exhibited the opposite pattern. There were marked differences between healthy samples and the two higher infection levels (moderate and severe infection) for Cab, Car, LAI, Deg, CCC, and Car/Cab. N and Ant only showed significant differences between the healthy samples and the highest infection level (severe infection). As Cab, Car, and Ant decreased, the spectral reflectance in the visible light region increased. The decrease in N and LAI was accompanied by a reduction in reflectance across the entire spectral range and the near-infrared area, which was exactly the opposite of Deg. The introduction of CFT greatly improved the accuracy of the WPM severity estimation model with R2 of 0.92. Features related to photosynthesis, pigment content, and canopy structure played a decisive role in estimating WPM severity. Also, results found that the feature importance showed a remarkable interchange as increasing disease levels. Using features that described canopy structure changes, such as optimized soil adjusted vegetation index, LAI, visible atmospherically resistant indices, and CCC, the mild infection stage of this disease was most easily distinguished from healthy samples. In contrast, most severe impacts of WPM were best characterized by features related to photosynthesis (e.g., photochemical reflectance index 515) and pigment content (e.g., normalized phaeophytinization index). This study help deepen the understanding of symptoms and spectral responses caused by WPM infection.