Integration of breeding innovations and epigenetic modifications offers the potential to boost productivity and promote sustainable agricultural practices, particularly in tomato production, which accounts for 16 % of global vegetable production. They are susceptible to various stress factors, Both abiotic (light, temperature, water, humidity, nutrients) and biotic (pests, diseases), which can impact fruit quality and reduce yield quantity by 50-70 %leading to food insecurity and economic losses. Climatic factors impact the traditional farming of tomatoes in the open field; innovative technologies aim to tackle the adverse effects of both abiotic and biotic stress factors. It highlights advancements in crop productivity and stress tolerance, including increased phytochemicals biosynthesis, improved water use efficiency, and soil salinity tolerance. However, challenges like photooxidative damage and downregulation of anthocyanin biosynthetic genes persist. This review provides highlights of promising technologies to mitigate the impact of stress factors on open field tomato production, highlighting both qualitative and quantitative losses. Besides sustainable systematic solutions, such as agroforestry systems, the advantages of using beneficial microbial endophytes, nanomaterials, and exogenous phytohormones in agriculture are discussed.

Large amounts of chemical fertilizers are still used to suppress pathogens and boost agricultural productivity and food generation. However, their use can cause harmful environmental imbalance. Furthermore, plants typically absorb limited amounts of the nutrients provided by chemical fertilizers. Recent studies are recommending the use of microbiota present in the soil in different formulations, considering that several microorganisms are found in nature in association with plants in a symbiotic, antagonistic, or synergistic way. This ecological alternative is positive because no undesirable significant alterations occur in the environment while stimulating plant nutrition development and protection against damage caused by control pathogens. Therefore, this review presents a comprehensive discussion regarding endophytic and rhizospheric microorganisms and their interaction with plants, including signaling and bio-control processes concerning the plant's defense against pathogenic spread. A discussion is provided about the importance of these bioinputs as a microbial resource that promotes plant development and their sustainable protection methods aiming to increase resilience in the agricultural system. In modern agriculture, the manipulation of bioinputs through Rhizobium contributes to reducing the effects of greenhouse gases by managing nitrogen runoff and decreasing nitrous oxide. Additionally, mycorrhizal fungi extend their root systems, providing plants with greater access to water and nutrients.

Heavy metal (HM) pollution in agricultural soils threatens plant growth and food security, underscoring the urgency for sustainable and eco-friendly solutions. This study investigates the potential of endophytic fungi, Fusarium proliferatum SL3 and Aspergillus terreus MGRF2, in mitigating nickel (Ni) and cadmium (Cd) stress in Solanum lycopersicum (tomato). These fungi were evaluated for their plant growth-promoting traits, including the production of indole-3-acetic acid (IAA) and siderophores, offering a sustainable strategy for alleviating HM toxicity. Inoculation with SL3 and MGRF2 significantly reduced metal accumulation in plant tissues by enhancing metal immobilization and modifying root architecture. Microscopic analysis revealed that fungi protected root epidermal cells from Ni- and Cd-induced damage, preserving cellular integrity and preventing plasmolysis. Fungal-treated plants exhibited improved growth and biomass, with SL3 demonstrating superior Cd stress mitigation and MGRF2 excelling under Ni stress. Photosynthetic pigment levels, including chlorophyll-a and carotenoids, were restored, highlighting the role of fungi in maintaining photosynthetic efficiency. Antioxidant activity was also modulated, as reduced glutathione (GSH) levels and increased flavonoid production were observed, contributing to enhanced oxidative stress management. Hormonal profiling revealed that fungal inoculation balanced stress-induced hormonal disruptions, with lower abscisic acid (ABA) levels and improved salicylic acid (SA) and gibberellic acid (GA) pathways. These changes facilitated better stress adaptation, enhanced nutrient uptake, and improved physiological performance. qRT-PCR analysis further revealed differential gene expression patterns, while antioxidant enzyme activity strengthened the plants' defense against HMinduced oxidative damage. Multivariate analyses highlighted shoot and root traits as critical indicators of resilience, with fungal inoculation driving substantial improvements. These findings demonstrate the potential of SL3 and MGRF2 as eco-friendly bioinoculants, offering a sustainable and cost-effective approach to reducing HMs toxicity in contaminated soils while enhancing crop productivity. This work highlights the promising role of plant-microbe interactions in advancing sustainable agriculture and addressing the challenges posed by heavy metal pollution.

Salt accumulation can degrade soil properties, decrease its productivity, and harm its ecological functions. Introducing salt-tolerant plant species associated with arbuscular mycorrhizal fungi (AMF) can act as an effective biological method for restoring salinized soils. AMF colonize plant roots and improve their nutrient acquisition capacity. However, there is limited knowledge on how AMF affects the production of signaling molecules, e.g., abscisic acid (ABA), salicylic acid (SA), and jasmonic acid (JA), related to plant-microbe interactions under salinity. Here, we assess the potential benefits of the AMF Rhizophagus intraradices in enhancing plant growth and nutrient uptake in addition to modulating stress hormone signaling levels (ABA, SA, and JA) of the facultative halophyte Sulla carnosa under saline conditions. Plants were grown in pots filled with soil and irrigated with 200 mM NaCl for 1 month. AMF symbiosis substantially increased the shoot dry weight (+107%), root dry weight (+67%), photosynthetic pigment content (chlorophyll a, chlorophyll b, and carotenoids), and nutrient uptake (C, N, P, K, Cu, and Zn) while significantly limiting the increase in the shoot Na+ concentration and H2O2 content caused by salinity stress. Mycorrhizal symbiosis significantly enhanced the root and shoot SA levels by 450% and 32%, respectively, compared to the stressed non-inoculated plants, potentially contributing to enhanced systemic resistance and osmotic adjustment under saline conditions. Salt stress increased the shoot ABA content, especially in R. intraradices-inoculated plants (113% higher than in stressed non-mycorrhizal plants). These findings confirm that AMF mitigated the adverse effects of salinity on S. carnosa by increasing the SA and ABA levels and reducing oxidative damage.

Drought is a serious environmental challenge that reduces the productivity of valuable crops, including wheat. Brassinosteroids (BRs) is a group of phytohormones that have been used to enhance wheat drought tolerance. Wheat cultivars with different adaptation strategies could have their own specific drought tolerance mechanisms, and could react differently to treatment with growth regulators. In this work, the effect of seed pretreatment with 0.4 mu M 24-epibrassinolide (EBR) was investigated in two wheat (Triticum aestivum L.) cultivars contrasting in drought behavior, tolerant Ekada 70 (cv. E70) and sensitive Zauralskaya Zhemchuzhina (cv. ZZh), in early ontogenesis under dehydration (PEG-6000) or soil drought conditions. EBR pretreatment mitigated the stress-induced inhibition of seedling emergence and growth, as well as membrane damage in cv.E70 but not in ZZh. An enzyme-linked immunosorbent assay (ELISA) revealed substantial changes in hormonal balance associated with ABA accumulation and a drop in the levels of IAA and cytokinins (CKs) in drought-subjected seedlings of both cultivars, especially ZZh. EBR-pretreatment reduced drought-induced hormone imbalance in cv. E70, while it did not have the same effect on ZZh. EBR-induced changes in the content of wheat germ agglutinin (WGA) belonging to the protective proteins in E70 seedlings suggest its contribution to EBR-dependent adaptive responses. The absence of a detectable protective effect of EBR on the ZZh cultivar may be associated with its insensitivity to pre-sowing EBR treatment.

The present study uncovers the impacts of pesticide-thiamethoxam (TMX- 750 mg L- 1 ) and salicylic acid (SA- 0.01, 0.1 and 1 mM) in Brassica juncea L. TMX poisoning exacerbates the nuclear and membrane damage, whereas an increment in the oxidative stress markers like hydrogen peroxide (H2O2), superoxide anions (O2- ) and malondialdehyde (MDA) contents has been observed. The significance of phytohormone SA in mitigating TMX toxicity by enhancing the growth, and antioxidant capacities of B. juncea seedlings is not well documented. Salicylic acid priming to these TMX-exposed seedlings maximizes the germination potential by 34%, and root, shoot length by 86.9% and 41.5%, whereas, minimizing the levels of oxidative stress indicators such as H2O2 by 34.8%, O 2- by 26.9% and amounts of MDA by 45.6% and EL (electrolyte leakage) contents by 22.7% under 1 mM of SA. Also, an increment in the activity of enzymatic antioxidants like superoxide dismutase (SOD), ascorbate peroxidase (APOX), glutathione peroxidase (GPOX), dehydroascorbate reductase (DHAR), glutathione reductase (GR), peroxidase (POD), and catalase (CAT) by 122.1%, 186%, 39%, 82.61%, 40.02%, 75.6% and 59.5% was observed when TMX exposed seeds were supplemented with the highest SA (1 mM) concentration. Whereas, an upregulation in the gene expressions of enzymatic antioxidants was assessed as well as a swift decrease in the RBOH1 (respiratory burst oxidase1) gene expression was observed under the subsequent SA supplementation. Thus, the results effectively address the ameliorative potentials of exogenously applied SA in order to maximize the growth and development, by mediating osmotic adjustments, and antioxidant potentials in B. juncea L.

Soil salinization is a severe environmental issue limiting the growth and yield of crops worldwide. Subsurface drip irrigation with micro-nano bubble hydrogen water (SDH) is an innovative way to realize the role of hydrogen gas (H2) in improving plant resistance to salt stress in practical agricultural productions. Nonetheless, limited information is available on how SDH affects the plant salt tolerance performance. Especially, the underlying physiological respond, hormone-regulated and soil microbial-mediated mechanisms have not been reported so far. In this study, the effects of SDH on lettuce (Lactuca sativa L.) growth, photosynthesis, root development, antioxidant system, phytohormone, and soil microbial community were investigated under normal and salt stress conditions. The results showed that, with salt stress, SDH significantly enhanced the lettuce fresh weight, photosynthesis activity, and root growth. The leaf antioxidant enzyme activities increased and reactive oxygen species (ROS) content decreased to reduce the oxidative damage. The decreased malondialdehyde (MDA) content indicated a low membrane lipid peroxidation responsible for cellular damage. SDH also helped to maintain osmotic homeostasis, which was reflected by the increased soluble protein (SP) content. Reduced Na+/ K+ ratio and ROS did not trigger excessive production of stress response hormones abscisic acid (ABA) and jasmonic acid (JA), which alleviated the mediated growth inhibition effects. SDH enriched the abundance of the plant growth-promoting rhizobacteria (PGPR) in the soil, such as Arthrobacter and Pseudomonas. That might be the reason for explaining the increase in hormone indoleacetic acid (IAA) in lettuce and 1-aminocyclopropane-1carboxylate (ACC) deaminase activity in the soil, which was beneficial for inhibiting ethylene production and promoting plant growth. Under the normal condition, variations of physiological and growth indicators as affected by SDH were similar to those under the salt stress condition, except for root development. High concentration of dissolved hydrogen gas in water might expel the oxygen. The induced soil anoxic environment limited oxygen diffusion, in turn inhibited root respiration and growth. The effect of hydrogen concentration on the plant tolerance against salt stress under different salt content could be further studied.

Soil salinization severely restricts the growth and development of crops globally, especially in the northwest Loess Plateau, where apples constitute a pillar industry. Nanomaterials, leveraging their unique properties, can facilitate the transport of nutrients to crops, thereby enhancing plant growth and development under stress conditions. To investigate the effects of nano zinc oxide (ZnO NP) on the growth and physiological characteristics of apple self-rooted rootstock M9-T337 seedlings under saline alkali stress, one-year-old M9-T337 seedlings were used as experimental materials and ZnO NPs were used as donors for pot experiment. Six treatments were set up: CK (normal growth), SA (saline alkali stress,100 mmol/L NaCl + NaHCO3), T1 (saline alkali stress + 50 mg/L ZnO NPs), T2 (saline alkali stress + 100 mg/L ZnO NPs), T3 (saline alkali stress + 150 mg/L ZnO NPs) and T4 (saline alkali stress + 200 mg/L ZnO NPs). The results were found to show that saline alkali stress could significantly inhibit the growth and development of M9-T337 seedlings, reduce photosynthetic characteristics, and cause ion accumulation to trigger osmotic regulation system, endogenous hormone and antioxidant system imbalances. However, the biomass, plant height, stem diameter, total leaf area and leaf perimeter of M9-T337 seedlings were significantly increased after ZnO NP treatment. Specifically speaking, ZnO NPs can improve the photosynthetic capacity of M9-T337 by increasing the content of photosynthetic pigment, regulating photosynthetic intensity and chlorophyll fluorescence parameters. ZnO NPs can balance the osmotic adjustment system by increasing the contents of soluble protein (SP), soluble sugar (SS), proline (Pro) and starch, and can also enhance the activities of enzymatic (SOD, POD, and CAT) and non-enzymatic antioxidant enzymes (APX, AAO, GR, and MDHAR) to enhance the scavenging ability of reactive oxygen species (H2O2, O2 center dot-), ultimately reducing oxidative damage; ZnO NPs promoted the growth of M9-T337 seedlings under saline alkali stress by synergistically responding to auxin (IAA), gibberellin (GA3), zeatin (ZT) and abscisic acid (ABA). Additionally, the Na+/K+ ratio was reduced by upregulating the expression of Na+ transporter genes (MdCAX5, MdCHX15, MdSOS1, and MdALT1) and downregulating the expression of K+ transporter genes (MdSKOR and MdNHX4). After comprehensive analysis of principal components and correlation, T3 (150 mg/L ZnO NPs) treatment possessed the best mitigation effect. In summary, 150 mg/L ZnO NPs(T3) can effectively maintain the hormone balance, osmotic balance and ion balance of plant cells by promoting the photosynthetic capacity of M9-T337 seedlings, and enhance the antioxidant defense mechanism, thereby improving the saline alkaline tolerance of M9-T337 seedlings.

To feed the nearly 10 billion people by the year 2050, agricultural activities and yield must be enhanced substantially, maintaining soil health and overpowering the expected adverse effects of climate change. High soil salinity is one of the major concerns in future farming, as salinity is a prominent abiotic stress that significantly impacts plants inhabiting arid and semiarid environments worldwide. The increasing levels of soil salinity are proving detrimental to agriculture, the general productivity of the ecosystem, and the economy at large. Excessive salt accumulation in plants leads to an osmotic imbalance, resulting in a decrease in photosynthesis, formation of reactive oxygen species, DNA damage, hormonal instability, and decreased water and mineral uptake. To mitigate the adverse impacts of salt stress, along with diverse physiological mechanisms, plants have developed symbiotic associations with endophytic microorganisms that reside within the plant tissues and help the plants in many ways. Endophytes have been found to alleviate the effects of salinity stress by diverse mechanisms-synthesis of osmolytes, and antioxidant enzymes such as catalase, superoxide dismutase, and peroxidase; synthesis and modulation of phytohormones such as ethylene, indole-3-acetic acid, gibberellin, abscisic acid, etc.; promotion of siderophore production and exopolysaccharide formation; carrying out nitrogen fixation, and increasing phosphate solubilization. In this review, the effects of salinity stress on plants, and the mechanisms by which endophytic microorganisms help the plants to withstand such stress are discussed at length. The application of tailored endophytic microbial consortia holds the key to future food security through sustainable agriculture.

Climate change not only leads to high temperatures, droughts, floods, storms and declining soil quality, but it also affects the spread and mutation of pests and diseases, which directly influences plant growth and constitutes a new challenge to food security. Numerous hormones like auxin, ethylene and melatonin, regulate plant growth and development as well as their resistance to environmental stresses. To mitigate the impact of diverse biotic and abiotic stressors on crops, single or multiple phytohormones in combination have been applied. Melatonin is a multifunctional signaling molecule engaged in the development and stress response of plants. In the current review, we discuss the synthesis and action of melatonin, as well as its utilization for plant resistance to different stresses from the perspective of practical application. Simultaneously, we elucidate the regulatory effects and complex mechanisms of melatonin and other plant hormones on the growth of plants, explore the practical applications of melatonin in combination with other phytohormones in crops. This will aid in the planning of management strategies to protect plants from damage caused by environmental stress.