Heavy metal contamination increases plant susceptibility to both biotic and abiotic stresses. However, the comprehensive impact of heavy metal pollution on plant hydraulics, which is crucial for plant productivity, and the interaction between heavy metal stress and environmental factors on plant health are not yet fully understood. In this study, we investigated the effects of cadmium exposure on plant-water relations and hydraulics of Solanum lycopersicum L., cultivar Piccadilly. Particular attention was given to leaf hydraulic conductance (KL) in response to cadmium pollution and dehydration. Cadmium exposure exhibited negligible impacts on tomato productivity but resulted in significant differences in pressure-volume derived traits. Leaves and roots of Cd-treated plants showed reduced wall stiffness compared to control samples. However, Cd-treated leaves had a less negative turgor loss point (Psi tlp), whereas Cd-treated roots exhibited more negative Psi tlp values due to lower osmotic potential at full turgor compared to control samples. Leaves and root cells of Cd-treated plants showed higher values of saturated water content compared to control plants, along with a distinct mineral profile between the two experimental groups. Despite similar leaf water potential thresholds for 50% and 80% loss of KL in control and cadmium-treated leaves, plants grown in cadmium-polluted soil showed higher leaf cell damages even under well watered conditions. This, in turn, affected the plant ability to recover from drought upon rehydration by compromising cell rehydration ability. Overall, the present findings suggest that under conditions of low water availability, cadmium pollution increases the risk of leaf hydraulic failure.

center dot Stomatal closure under high VPDL (leaf to air vapour pressure deficit) is a primary means by which plants prevent large excursions in transpiration rate and leaf water potential (Psi(leaf)) that could lead to tissue damage. Yet, the drivers of this response remain controversial. Changes in Psi(leaf) appear to drive stomatal VPDL response, but many argue that dynamic changes in soil-to-leaf hydraulic conductance (Ks-l) make an important contribution to this response pathway, even in well-hydrated soils. center dot Here, we examined whether the regulation of whole plant stomatal conductance (g(c)) in response to typical changes in daytime VPDL is influenced by dynamic changes in Ks-l. We use well-watered plants of two species with contrasting ecological and physiological features: the herbaceous Arabidopsis thaliana (ecotype Columbia-0) and the dry forest conifer Callitris rhomboidea. center dot The dynamics of Ks-l and g(c) were continuously monitored by combining concurrent in situ measurements of Psi(leaf) using an open optical dendrometer and whole plant transpiration using a balance. Large changes in VPDL were imposed to induce stomatal closure and observe the impact on Ks-l. center dot In both species, g(c) was observed to decline substantially as VPDL increased, while Ks-l remained stable. Our finding suggests that stomatal regulation of transpiration is not contingent on a decrease in Ks-l. Static Ks-l provides a much simpler explanation for transpiration control in hydrated plants and enables simplified modelling and new methods for monitoring plant water use in the field.

Water stress can trigger acclimation responses and damage plants. The aim of this study was to evaluate the integrative responses of cotton hydraulic conductance, leaf photosynthesis, and carbon metabolism to short-term drought and subsequent rewatering. A water-controlled pot experiment was conducted in 2020, with soil water drying continuing for one day (D1), two days (D2), and three days (D3) after it reached 40% +/- 5% of the soil water holding capacity at the blooming stage of cotton, and the soil was then rewatered to the soil water holding capacity. We investigated how the stem hydraulic conductance, gas exchange, and biochemical traits of cotton were affected by imposed drought stress and subsequent rewatering. The hydraulic characteristics of cotton in the D2 and D3 treatments evolved with damage, complete closure of stomatal conductance, and complete deterioration of photosynthesis, in addition to severe floating changes in the carbon metabolism affected by drought. The leaves' functional characteristics after rewatering cannot be completely recovered to full-irrigation levels, and the recovery extent was strongly linked to the duration. Consequently, it is considered desirable to maintain normal physiological activity during the cotton reproductive period, and the drought episode can be sustained for 1 day in a long-term perspective when the soil water content is depleted to 40% +/- 5% of the soil water holding capacity. These results can provide in-depth ideas for better understanding the hydraulic and physiological responses of cotton to drought episodes and rewatering, and they can help drought-affected cotton to cope with future climate change.