Climate change events significantly impact the food production chain by damaging crops in their most fragile phenological states. Furthermore, increasing human population and excess food waste present agricultural systems with the challenge of closing the yield gap and securing food demands in the future as well as protect the soil health and biodiversity. Biostimulants are a novel alternative in agriculture that can effectively use inputs, enhance crop resilience to abiotic stresses and improve food quality. Additionally, biostimulants offer a promising and eco-friendly solution for reducing the use of chemical fertilizers, as they have the potential to increase crop nutrient use efficiency and yield. Because of their effects on plant growth, a wide range of products can be marketed as biostimulants. Presented in this review is an overview of recent literature on the use of plant growth-promoting microbes and microalgae-derived extracts obtained from either waste streams or recycled substrates. Starting from their source material, extraction technologies and application modalities, a view of their factors shaping the composition and activity of biostimulants is provided to elucidate a mechanistic model of action which leads to increased stress resilience in crops. This work further sets out to understand if the biostimulants can be used to transform waste into a valuable product that can accelerate the transition to sustainable agriculture.This article is part of the theme issue 'Crops under stress: can we mitigate the impacts of climate change on agriculture and launch the 'Resilience Revolution'?'.

Fungal diseases caused by Fusarium spp. significantly threaten food security and sustainable agriculture. One of the traditional strategies for eradicating Fusarium spp. incidents is the use of chemical and synthetic fungicides. The excessive use of these products generates environmental damage and has negative effects on crop yield. It puts plants in stressful conditions, kills the natural soil microbiome, and makes phytopathogenic fungi resistant. Finally, it also causes health problems in farmers. This drives the search for and selection of natural alternatives, such as bio-fungicides. Among natural products, algae and cyanobacteria are promising sources of antifungal bio-compounds. These organisms can synthesize different bioactive molecules, such as fatty acids, phenolic acids, and some volatile organic compounds with antifungal activity, which can damage the fungal cell membrane that surrounds the hyphae and spores, either by solubilization or by making them porous and disrupted. Research in this area is still developing, but significant progress has been made in the identification of the compounds with potential for controlling this important pathogen. Therefore, this review focuses on the knowledge about the mechanisms of action of the fatty acids from macroalgae, microalgae, and cyanobacteria as principal biomolecules with antifungal activity, as well as on the benefits and challenges of applying these natural metabolites against Fusarium spp. to achieve sustainable agriculture.

Salvia miltiorrhiza , a widely used medicinal herb renowned for its properties in promoting blood circulation, removing blood stasis and alleviating pain, is currently facing quality degradation due to excessive heavy metal levels, posing a threat to medication safety. In order to investigate the effects of microbial inoculant, microalgae and biochar on the growth of Salvia miltiorrhiza under copper (Cu) stress, as well as its Cu absorption, antioxidant activity, active component contents and rhizosphere microbial community, a pot experiment was conducted. Salvia miltiorrhiza plants were cultivated in the soil containing 400 mg/kg of Cu for six months and treated with microbial inoculant, microalgae and biochar, either individually or in combination. Almost all soil amendment treatments led to an increase in root biomass. Notably, co -application of microbial inoculant and microalgae had the optimal effect with a 63.07 % increase compared to the group treated solely with Cu. Moreover, when microbial inoculant was applied alone or in combination with microalgae, the Cu content in plant roots was reduced by 19.29 % and 25.37 %, respectively, whereas other treatments failed to show a decreasing trend. Intriguingly, Cu stress increased the active component contents in plant roots, and they could also be enhanced beyond non -stress levels when microbial inoculant and microalgae were applied together or in combination with biochar. Analyses of plant antioxidant activity, soil properties and rhizosphere microorganisms indicated that these amendments may alleviate Cu stress by enhancing peroxidase activity, facilitating plant nutrient absorption, and enriching beneficial microorganisms capable of promoting plant growth and mitigating heavy metal induced damage. This study suggests that the combined application of microbial inoculant and microalgae can reduce Cu levels in Salvia miltiorrhiza while enhancing its quality under Cu stress.

Heavy metal contamination negatively affects plants and animals in water as well as soils. Some microalgae can remove heavy metal contaminants from wastewater. The aim of this study was to screen green microalgae (GM) to identify those that tolerate high concentrations of toxic heavy metals in water as possible candidates for phytoremediation. Analyses of the tolerance, physiological parameters, ultrastructure, and transcriptomes of GM under Cd/Pb treatments were conducted. Compared with the other GM, Chlorella pyrenoidosa showed stronger tolerance to high concentrations of Cd/Pb. The reduced glutathione content and peroxidase activity were higher in C. pyrenoidosa than those in the other GM. Ultrastructural observations showed that, compared with other GM, C. pyrenoidosa had less damage to the cell surface and interior under Cd/Pb toxicity. Transcriptome analyses indicated that the peroxisome and sulfur metabolism pathways were enriched with differentially expressed genes under Cd/Pb treatments, and that CpSAT, CpSBP, CpKAT2, Cp2HPCL, CpACOX, CpACOX2, and CpACOX4, all of which encode antioxidant enzymes, were up-regulated under Cd/Pb treatments. These results show that C. pyrenoidosa has potential applications in the remediation of polluted water, and indicate that antioxidant enzymes contribute to Cd/Pb detoxification. These findings will be useful for producing algal strains for the purpose of bioremediation in water contamination.