Expansive soil, characterized by significant swelling-shrinkage behavior, is prone to cracking under wet-dry cycles, severely compromising engineering stability. This study combines experimental and molecular dynamics (MD) simulation approaches to systematically investigate the improvement effects and micromechanisms of polyvinyl alcohol (PVA) on expansive soil. First, direct shear tests were conducted to analyze the effects of PVA content (0 %-4 %) and moisture content (30 %-50 %) on the shear strength, cohesive force, and internal friction angle of modified soil. Results show that PVA significantly enhances soil cohesive force, with optimal improvement achieved at 3 % PVA content. Second, wet-dry cycle experiments revealed that PVA effectively suppresses crack propagation by improving tensile strength and water retention. Finally, molecular dynamics simulations uncovered the distribution of PVA between montmorillonite (MMT) layers and its influence on interfacial friction behavior. The simulations demonstrated that PVA forms hydrogen bonding networks, enhancing interlayer interactions and frictional resistance. The improved mechanical performance of PVAmodified soil is attributed to both nanoscale bonding effects and macroscale structural reinforcement. This study provides theoretical insights and technical support for expansive soil stabilization.

Nanoplastics (NPs) and zinc (Zn), both widespread in soil environments, present considerable risks to soil biota. While NPs persist environmentally and act as vectors for heavy metals like Zn, their combined toxicity, especially in soil invertebrates, remains poorly understood. This study evaluates the individual and combined effects of Zn and NPs on earthworm coelomocytes and explores their interactions with Cu/Zn-superoxide dismutase (SOD), an antioxidant enzyme. Molecular docking revealed that NPs bind near the active site of SOD through pi-cation interactions with lysine residues, further stabilized by neighboring hydrophobic amino acids. Viability assays indicated that NPs alone (20 mg/L) had negligible impact (94.54 %, p > 0.05), Zn alone (300 mg/L) reduced viability to 80.02 %, while co-exposure reduced it further to 73.16 %. Elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) levels were elevated to 186 % and 173 % under co-exposure, alongside greater antioxidant enzyme disruption, point to synergistic toxicity. Dynamic light scattering and zeta potential (From -13 to -7 mV) analyses revealed larger particle sizes in the combined system, indicative of enhanced protein interactions. Conformational changes in SOD, such as alpha-helix loss and altered fluorescence, further support structural disruption. These findings demonstrate that co-exposure to NPs and Zn intensifies cellular and protein-level toxicity via integrated physical and biochemical mechanisms, providing critical insight into the ecological risks posed by such co-contaminants in soil environments.

Friction characteristics are critical mechanical properties of clay, playing a pivotal role in the structural stability of cohesive soils. In this study, molecular dynamics simulations were employed to investigate the shear behavior of undrained montmorillonite (MMT) nanopores with varying surface charges and interlayer cations (Na+, K+, Ca2+), subjected to different normal loads and sliding velocities. Consistent with previous findings, our results confirm that shear stress increases with normal load. However, the normal load-shear stress curves reveal two distinct linear regions, indicating segmented friction behavior. Remarkably, the friction coefficient declines sharply beyond a critical pressure point, ranging from 5 to 7.5 GPa, while cohesion follows an inverse trend. The elevated friction coefficient at lower pressures is attributed to the enhanced formation of hydrogen bonds and concomitant changes in density distribution. Furthermore, shear strength was observed to increase with sliding velocities, normal loads, and surface charges, with Na-MMT exhibiting superior shear strength compared to KMMT and Ca-MMT. Interestingly, the friction coefficient shows a slight decrease with increasing surface charge, while ion type exerts a minimal effect. In contrast, cohesion is predominantly influenced by surface charge and remains largely unaffected by ion type, except under extreme pressures and velocities.

In view of the pollution of unpaved road dust in the current mines, this study demonstrated the excellent dust suppression performance of the dust suppressant by testing the dynamic viscosity, penetration depth and mechanical properties of the dust suppressant, and apply molecular dynamics simulations to reveal the interactions between substances. The results showed that the maximum dust suppression rate was 97.75 % with a dust suppressant formulation of 0.1 wt% SPI + 0.03 wt% Paas + NaOH. The addition of NaOH disrupts the hydrogen bonds between SPI molecules, which allows the SPN to better penetrate the soil particles and form effective bonding networks. The SPI molecules rapidly absorb onto the surface of soil particles through electrostatic interactions and hydrogen bonds. The crosslinking between SPI molecules connects multiple soil particles, forming larger agglomerates. The polar side chain groups in the SPN interact with soil particles through dipole-dipole interactions, further stabilizing the agglomerates and resulting in an enhanced dust suppression effect. Soil samples treated with SPN exhibited higher compressive strength values. This is primarily attributed to the stable network structure formed by the SPN dust suppressant within the soil. Additionally, the SPI molecules and sodium polyacrylate (Paas) molecules in SPN contain multiple active groups, which interact under the influence of NaOH, restricting the rotation and movement of molecular chains. From a microscopic perspective, the SPN dust suppressant further strengthens the interactions between soil particles through mechanisms such as liquid bridge forces, which contribute to the superior dust suppression effect at the macroscopic level.

Most Australian vegetable growers apply fumigants or nematicides as a precautionary nematode control measure when crops susceptible to root-knot nematode (RKN, Meloidogyne spp.) are grown in soils and environmental conditions suitable for the nematode. The only way growers can make rational decisions on whether these expensive and environmentally disruptive chemicals are required is to regularly monitor RKN populations and decide whether numbers prior to planting are high enough to cause economic damage. However, such monitoring programs are difficult to implement because nematode quantification methods vary in efficiency and the damage threshold for RKN on highly susceptible vegetable crops is often < 10 root-knot nematodes /200 mL soil. Consequently, five nematode quantification methods were tested to see whether they could reliably detect these very low population densities of RKN. Two novel methods produced consistent results: 1) extracting nematodes from 2 L soil samples using Whitehead trays, quantifying the RKN DNA in the nematode suspension using molecular methods, and generating a standard curve so that the molecular results provided an estimate of the total number of RKN individuals in the sample, and 2) a bioassay in which two tomato seedlings were planted in pots containing 2 L soil and the number of galls produced on roots were counted after 21-25 days. Both methods could be used to quantify low populations of RKN, but bioassays are more practical because expensive equipment and facilities are not required and they can be done at a local level by people lacking nematological or molecular skills.

Background and AimsGlobal climate change is intensifying the co-occurrence of abiotic stresses, particularly combined waterlogging/submergence and salinity, posing severe and escalating threats to woody plant ecosystems critical for biodiversity, carbon storage, and soil stabilization. Despite extensive research on herbaceous species, understanding of woody plant responses remains fragmented and disproportionately focused on specific groups like mangroves and halophytes. This review aims to synthesize and critically evaluate the current state of knowledge on the integrated physiological, morphological, and molecular responses of diverse woody plants to this challenging combined stress scenario.MethodsA comprehensive synthesis and analysis of existing scientific literature was conducted. This involved systematically examining empirical studies, comparative analyses, and theoretical frameworks related to the responses of various woody plant species to the concurrent application of waterlogging/submergence and salinity stress, drawing comparisons to single-stress effects and herbaceous model systems.ResultsThe majority of woody plants exhibit synergistic, more detrimental effects under combined stress compared to either stress alone. Key manifestations include significantly heightened inhibition of photosynthesis, severe disruption of ion (particularly Na+ and Cl-) homeostasis leading to toxicity, and exacerbated oxidative damage. Woody plants utilize core stress tolerance mechanisms analogous to herbaceous species, such as ion exclusion/compartmentalization, activation of enzymatic and non-enzymatic antioxidant systems, and osmotic adjustment via compatible solute accumulation. Crucially, they also deploy distinctive structural and long-term adaptive strategies, including the development of specialized organs (pneumatophores, hypertrophic lenticels), deep root systems for accessing less saline groundwater, and physiological acclimation processes leveraging their perennial nature. Nevertheless, critical knowledge gaps persist, particularly concerning the underlying molecular signaling networks, the mechanisms of long-term adaptation over years/decades, and the specific responses of mature trees in natural ecosystems.ConclusionSignificant gaps hinder a comprehensive understanding of how woody plants cope with combined waterlogging/submergence and salinity stress. To advance fundamental knowledge and inform effective ecological restoration strategies for climate-resilient landscapes, future research must prioritize the application of integrated multi-omics approaches (genomics, transcriptomics, proteomics, metabolomics), the development of high-efficiency genetic transformation techniques for recalcitrant woody species, the deployment of advanced high-throughput phenotyping platforms, and crucially, long-term field-based studies simulating realistic future stress scenarios.

To overcome the limitations of microscale experimental techniques and molecular dynamics (MD) simulations, a coarse-grained molecular dynamics (CGMD) method was used to simulate the wetting processes of clay aggregates. Based on the evolution of swelling stress, final dry density, water distribution, and clay arrangements under different target water contents and dry densities, a relationship between the swelling behaviors and microstructures was established. The simulated results showed that when the clay-water well depth was 300 kcal/mol, the basal spacing from CGMD was consistent with the X-ray diffraction (XRD) data. The effect of initial dry density on swelling stress was more pronounced than that of water content. The anisotropic swelling characteristics of the aggregates are related to the proportion of horizontally oriented clay mineral layers. The swelling stress was found to depend on the distribution of tactoids at the microscopic level. At lower initial dry density, the distribution of tactoids was mainly controlled by water distribution. With increase in the bound water content, the basal spacing expanded, and the swelling stresses increased. Free water dominated at higher water contents, and the particles were easily rotated, leading to a decrease in the number of large tactoids. At higher dry densities, the distances between the clay mineral layers decreased, and the movement was limited. When bound water enters the interlayers, there is a significant increase in interparticle repulsive forces, resulting in a greater number of small-sized tactoids. Eventually, a well-defined logarithmic relationship was observed between the swelling stress and the total number of tactoids. These findings contribute to a better understanding of coupled macro-micro swelling behaviors of montmorillonite-based materials, filling a study gap in clay-water interactions on a micro scale. (c) 2025 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/ 4.0/).

The mechanical behavior of expansive soil in geotechnical engineering is significantly sensitive to loading rates, hydration, confining pressure, etc., where most engineering problems are attributed to the existence of montmorillonite in expansive soil. Here, the hydration, confining pressure, and loading rate effect on the mechanical behavior of montmorillonite were investigated through the triaxial tests and molecular dynamics (MD) simulation method, revealing their fundamental mechanism between the microscale and macroscale. The average basal spacing of hydrated montmorillonite system, the diffusion coefficient and density distribution of interlayer water molecules were calculated for the verification of MD model. The experimental results indicated that the stress-strain relationship of montmorillonite was the strain-hardening type. The failure stress did not increase monotonously with the increase in loading rate, and there were two obvious critical points. The failure stress of the soil sample increased with the increase of the confining pressure, and the decrease of the water content, where their fundamental mechanism between microscale and macroscale were adequately discussed. Furthermore, the stress-strain response, total energy evolution, deformation evolution of atomistic structure, and broken bonds evolution were analyzed to deeply understand the fundamental deformation mechanism at the microscale. The multi-scale studies could effectively examine the macroscopic mechanical behavior of expansive soil and elucidate its microscopic mechanisms.

Rubber-based intercropping is a recommended practice due to its ecological and economic benefits. Understanding the implications of ecophysiological changes in intercropping farms on the production and technological properties of Hevea rubber is still necessary. This study investigated the effects of seasonal changes in the leaf area index (LAI) and soil moisture content (SMC) of rubber-based intercropping farms (RBIFs) on the latex biochemical composition, yield, and technological properties of Hevea rubber. Three RBIFs: rubber-bamboo (RB); rubber-melinjo (RM); rubber-coffee (RC), and one rubber monocropping farm (RR) were selected in a village in southern Thailand. Data were collected from September to December 2020 (S1), January to April 2021 (S2), and May to August 2021 (S3). Over the study period, RB, RM, and RC exhibited significantly high LAI values of 1.2, 1.05, and 0.99, respectively, whereas RR had a low LAI of 0.79. The increasing SMC with soil depths was pronounced in all RBIFs. RB and RM expressed less physiological stress and delivered latex yield, which was on average 40% higher than that of RR. With higher molecular weight distributions, their rheological properties were comparable to those of RR. However, the latex Mg content of RB and RM significantly increased to 660 and 742 mg/kg, respectively, in S2. Their dry rubbers had an ash content of more than 0.6% in S3.

The effective stress principle is the fundamental theory of soil mechanics. The effective stress transmitted between particles dominates the mechanical properties of soil, such as strength, deformation, and drainage. However, there remains a paucity of research on the effective stress in the compression of nano-scale clay minerals. This study explored the application of the effective stress principle in the consolidation behavior of kaolinite through the Molecular Dynamics method. The calibration and correction for micro effective stress and pore water pressure were first proposed. Micro-effective stress is the stress on the mineral itself in the contact part of two particles, while micro-pore water pressure always represents that on the weakly bound and free water in the same part. The strongly bound water film between particles can indirectly transmit the micro-effective stress through the electrical double-layer repulsion. The calculation of micro stress has been corrected according to the derivation of macro theory, and the results obtained corresponded well with that in the macro experiment. Moreover, the evolution of effective stress was analyzed by observing the interparticle water film. The increase in effective stress during consolidation was mainly due to the compression of the strongly bound water and the drainage of weakly bound and free water.