Legumes are a vital component of agriculture, providing essential nutrients to both humans and soil through their ability to fix atmospheric nitrogen. However, the production of legume crops is often hindered by various biotic and abiotic stresses, limiting their yield and nutritional quality of crops by damaging plant tissues, which can result in lower protein content, reduced levels of essential vitamins and minerals, and compromised seed quality. This review discusses the recent advancements in technologies that are revolutionizing the field of legume crop improvement. Genetic engineering has played a pivotal role enhancing legume productivity. Through the introduction of genes encoding for enzymes involved in nitrogen fixation, leading to higher yields and reducing the reliance on synthetic fertilizers. Additionally, the incorporation of genes conferring disease and pest resistance has significantly reduced the need for chemical pesticides, making legume cultivation more sustainable and environmentally friendly. Genome editing technologies, such as CRISPR-Cas9, have opened new avenues for precision breeding in legumes. Marker-assisted selection and genomic selection are other powerful tools that have accelerated the breeding process. These techniques have significantly reduced time and resources required to develop new legume varieties. Finally, advancement technologies for legume crop improvement are aid and enhancing the sustainability, productivity, and nutritional quality of legume crops.

In recent years, excessive accumulations of iron (Fe), manganese (Mn), and nitrogen (N) have been observed in the groundwater of agricultural regions, particularly in flood irrigation areas. Nevertheless, the causes of this phenomenon and the associated hydrobiogeochemical processes remain elusive. This study demonstrated that redox fluctuations instigated by flood irrigation triggered a synergistic interaction between the N cycles and the activation of Fe and Mn oxides, thereby resulting in elevated concentrations of Fe, Mn, and N simultaneously. Static experiments revealed that the properties of the topsoil exerted a profound influence on the N induced release of Fe and Mn. The black soil (TFe: 1.5-2.3 times, Mn(II): 1.1-1.5 times, nitrate: 1.3-1.4 times) had greater release potential than meadow and dark brown soils due to higher electron donors/acceptors and substrates. Dynamic column experiments further elucidated that the wet-dry cycles induced by agricultural cultivation regulated the release process through the formation of zonal redox gradients and the structuring of microbial community. Organic nitrogen mineralization, chemolithotrophic nitrification, and Feammox/Mnammox were identified as the primary mechanisms responsible for the reductive dissolution of Fe-Mn oxides. On the other hand, autotrophic denitrification, with nitrate serving as the electron acceptor, constituted the main process for the reoxidation of Fe and Mn. Furthermore, the agricultural activities exerted a significant impact on the nitrate attenuation process, ultimately resulting in the recurrence of TFe (black soil: 1.5-6.3 times) and nitrate (black soil: 1.4-1.6 times) pollution during the phase after harvesting of rice (days 40-45) in saturated zone. The findings of this study not only deepened the understanding of the intricate interactions and coupled cycles between primary geochemical compositions and anthropogenic pollutants, but also provided a scientific foundation for the effective management and prevention of groundwater pollution stemming from agricultural cultivation processes.

Moderate nitrogen addition can enhance plant growth performance under salt stress. However, the regulatory effects of nitrogen addition on the growth of the leguminous halophyte medicinal plant, Sophora alopecuroides, under salt stress remain unclear. In this study, a two-factor pot experiment with different NaCl levels (1 g/kg, 2 g/kg, 4 g/kg) and NH4NO3 levels (0 mg/kg, 32 mg/kg, 64 mg/kg, 128 mg/kg) was set up to systematically study the response of S. alopecuroides plant phenotype, nodulation and nitrogen fixation characteristics, nitrogen (N), phosphorus (P), potassium (K) nutrient absorption and utilization efficiency, plant biomass and nutrient accumulation to nitrogen addition under salt stress. The results demonstrated that under mild (1 g/kg NaCl) and moderate (2 g/kg NaCl) salt stress, S. alopecuroides exhibited a relatively low nitrogen demand. Specifically, low (32 mg/kg N) and medium (64 mg/kg N) nitrogen levels significantly enhanced nodule nitrogenase activity and nitrogen fixation capacity. Furthermore, the uptake of essential nutrients, including N, P, and K, in the aboveground biomass was markedly increased, which in turn promoted the accumulation of major nutrients such as crude protein, crude fat, and alkaloids, as well as overall biomass production. However, under severe (4 g/kg NaCl) salt stress, S. alopecuroides exhibited a preference for low nitrogen levels (32 mg/kg N). Under S3 conditions, excessive nitrogen application (e.g., 64 mg/kg and 128 mg/kg N) exacerbated the damage caused by salt stress, leading to significant inhibition of nitrogen fixation and nutrient uptake. Consequently, this resulted in a substantial reduction in biomass. This study provides a theoretical basis for nitrogen nutrition management of S. alopecuroides under salt stress conditions and offers valuable insights for optimizing fertilization and nutrient management strategies in saline-alkali agricultural production.

Nitrogen is an essential element for life but its excessive release into the environment in the form of reactive nitrogen causes severe damage, including acidification and eutrophication. One of the main sources of nitrogen pollution is the use of fertilizers in agricultural soils. Feammox is a recently described pathway that couples ammonium (NH4+) oxidation with iron (Fe) reduction. In this study, the enrichment and bioaugmentation of anaerobic sludge under conditions that promote Feammox activity were investigated. The first enrichment stage (E1) achieved 28% of ammonium removal after 28 days of incubation, with a production of 30 mg/L of Fe2+. E1 was then used as inoculum for two enrichments at 35 degrees C with different carbon sources: sodium acetate (E2) and sodium bicarbonate (E3). Neither E2 nor E3 showed significant NH4+ removal, but E2 was highly effective in iron reduction, reaching Fe2+ concentrations of 110 mg/L. Additionally, an increase in nitrate (NO3-) concentration was observed, which may indicate the occurrence of this pathway in the Feammox process. The Monod kinetic model, analyzed using AQUASIM software, showed a good fit to the experimental data for NH4+, NO3-, and Fe2+. Sequencing analysis revealed the presence of phyla associated with Feammox activity. Although there was only a slight difference in NH4+ removal between the bioaugmented and non-augmented control sludge, the bioaugmented sludge was statistically superior in nitrate production and iron reduction. This study provides valuable insights into the enrichment and bioaugmentation of the Feammox process potential large-scale wastewater treatment applications.

Periphyton-based biofertilizer have a high potential for soil remediation, particularly for controlling soil salinization. This global environmental problem leads to low soil utilization and insufficient crop yields. Efficient and sustainable methods of managing saline soils are needed to reduce salinization and improve soil fertility and crop quality. Traditional methods such as physical mulching and chemical amendments, while improving soil conditions, exhibit limited effectiveness and may damage soil structure. This study aims to evaluate the feasibility of algae-based fertilizers in remediating saline-alkali soils and improving crop performance. The review delves into the and application prospects of algae-based fertilizers, highlighting their potential from both sustainable development and economic perspectives. It further advocates integrating other emerging technologies with the production and application of algae-based fertilizers to address the increasingly severe challenges posed by degraded soil resources and environmental instability. The review found that algal fertilizers are more environmentally friendly than traditional chemical fertilizers but are not inferior in function. This approach offers more efficient and sustainable solutions for managing saline-alkaline soils and effectively achieves sus-tainable agricultural production. Furthermore, it is necessary to conduct experimental research and monitoring evaluations of algal fertilizers to formulate scientific and rational fertilization plans to meet the increasingly serious challenges facing soil resources and unstable environments. The findings of this study will provide theoretical and technical support for using algae biofertilizers for soil remediation, improving crop quality and sequestering carbon.

When processing barley for the brewing and food industries, one of the process steps is the separation of barley grains. After separation, waste fractions are created that contain damaged grains, dust, stones, grass, clay residues, etc. The waste fractions therefore contain recyclable components. It is important to find a suitable way to use these waste fractions. This research is focused on the possibilities of applying torrefied waste fractions as soil enrichment to support plant growth. The first waste fraction contained lower-quality barley grains that are used as feed for livestock. The second waste fraction contained grass seeds and chaff and can be used as feed for forest animals. The third waste fraction, which contained aspiration dust, is currently being incinerated and disposed at biogas plants. Experiments were conducted with different ratios of torrefied fractions added to the soil and the values of total nitrogen were analyzed as an indicator of the benefit to the soil. The results showed that torrefied waste fractions exhibit positive properties for plant growth. The best results were achieved with a mixture containing 10 and 50 % of soil enriched with torrefied second and third waste fractions. Experiments confirmed a positive effect on plant growth, which suggests the possibility of applying this procedure in practice. Compared to current research, this method can contribute to the sustainable management of biowaste and its effective use for improving soil conditions with a high potential for sustainable agriculture.

Drought and soil nitrogen (N) deficiency are the limiting factors for poplar plantation productivity improvement in semi-arid regions. N addition could alleviate the growth decline of trees caused by drought; however, the effectiveness under severe drought and the underlying ecophysiological understanding remains uncertain. To further clarify the mechanisms of N addition in regulating tree biomass accumulation under different drought levels, we investigated the effects of 6 g NH4NO3 per plant addition on the carbon and N assimilation and biomass accumulation of potted poplar seedlings under moderate or severe drought (40 % or 20 % of field capacity) conditions, with a particular emphasis on carbon and N interactions. We found that under moderate drought, N addition markedly promoted the activities of antioxidases, nitrate reductase (39 %), and N concentration (56 %) in leaves, significantly alleviated the damages of the membranes and photosystem II, and increased both leaf area (69 %) and chlorophyll content per unit leaf area, along with net photosynthesis rate (34 %), thereby significantly alleviating growth restrictions. However, under severe drought, although N addition increased the accumulation of both soluble sugars and N of the whole plant, it did not ameliorate the damage to membranes and photosystem II, nor did it improve chlorophyll content, leaf area, or biomass accumulation. Therefore, N addition could increase leaf area, enhance antioxidants, and positively influence leaf carbon assimilation (0.60, p < 0.001) in poplar seedlings under moderate drought. The restrictions on leaf area and carbon assimilation were exacerbated during severe drought, which mitigated the positive effects of N addition on carbon assimilation and biomass accumulation. The findings of this study suggest that the growth of hybrid poplar can be enhanced by applying N fertilizer under mild drought conditions. In contrast, N fertilization has no significant effect in severe drought conditions.

Thermal damage mechanisms are crucial in reservoir stimulation for enhanced geothermal system (EGS). This study investigates the thermal damage mechanisms in granite samples from the Gonghe Basin, Qinghai, China. The granite samples were heated to 400 degrees C and then cooled in air, water, or liquid nitrogen. The physical and mechanical properties of the thermally treated granite were evaluated, and microstructural changes were analyzed using a scanning electron microscope (SEM) and computed tomography (CT). The results indicate that cooling with water and liquid nitrogen significantly enhances permeability and brittleness while reducing P-wave velocity, strength, and Young's modulus. Specifically, liquid nitrogen cooling increased granite permeability by a factor of 5.24 compared to the untreated samples, while reducing compressive strength by 13.6%. After thermal treatment, the failure mode of the granite shifted from axial splitting to a combination of shear and tension. Microstructural analysis revealed that liquid nitrogen-cooled samples exhibited greater fracture complexity than those cooled with water or air. Additionally, acoustic emission (AE) monitoring during damage evolution showed that liquid nitrogen cooling led to higher cumulative AE energy and a lower maximum AE energy rate, with numerous AE signals detected during both stable and unstable crack growth. The results suggest that liquid nitrogen induces a stronger thermal shock, leading to more significant thermal damage and promoting the development of a complex fracture network during EGS reservoir stimulation. This enhances both the heat exchange area and the permeability of the deep hot dry rock (HDR) in EGS reservoirs. The insights from this study contribute to a deeper understanding of thermal damage characteristics induced by different cooling media and provide valuable guidance for optimizing deep geothermal energy extraction. (c) 2025 Institute of Rock and Soil Mechanics, Chinese Academy of Sciences. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

The adoption of sustainable farming practices will improve food security around the world. The evidence that food is produced sustainably has become important for maintaining access to global markets and is influencing commodity marketing and pricing. This paper explores the current state of global sustainability reporting and examines whether yield data could improve the sustainability of farming by adding more rigour and transparency to the evidential basis of sustainability. The Australian grains and oilseeds industry is used as a case study with most of the Australian grain and oilseed crop grown for export markets. Sustainability policies in the European Union, United States of America and Australia are contrasted, with a focus on the improved management of nitrogenous fertiliser, which is viewed as the most efficient way to reduce the environmental impact of agriculture. Generally, sustainability reporting is based on a suite of indicators that are easy to measure and interpret, sensitive to change, technically sound and cost-effective. These indicators serve as a mechanism to quantify and document the practices used to produce crops but some of the current measures are relatively coarse and lack transparency. The time and cost incurred to collect these measurements could be reduced by using secondary data to report on sustainability. Yield data are already collected by many grain, and oilseed growers, and provide a transparent, evidence-based way to optimise and report on fertiliser application at fine scale. Yield data can help to maintain soil health and farm profit, reduce environmental damage and generate quantitative data for reporting on agricultural sustainability, but some challenges remain before it could be implemented as a universal reporting measure.

Soil nitrogen-hydrolyzing enzymes catalyzes a key rate-limiting step in regulating the circulation of soil nutrient elements. The response of soil nitrogen (N)-hydrolyzing enzyme activities to environmental changes has been investigated in different geographic scales or ecosystems. Global warming has increased the frequency of soil freeze-thaw (FT) events, resulting in drastic changes in soil enzyme activities. Clarifying the changes in soil N-hydrolyzing enzymes under freeze-thaw conditions is essential for improving the N cycling and utilization efficiency in soil. However, how soil N-hydrolyzing enzymes respond to FT remains unclear. This study was aimed to analyze the influence of FT on soil N-hydrolyzing enzyme activity in Mollisols. The results showed that soil physicochemical properties and enzyme activities were changed after freeze-thaw events, and freeze-thaw temperature (FTF) had a greater impact on these properties than the number of freeze-thaw cycles (FTC). Correlation analysis showed that total organic carbon (TOC), total nitrogen (TN), total phosphorus (TP) and pH were the major factors affecting enzyme activities in FT events. Soil N-hydrolyzing enzyme activity was mainly regulated by environmental factors, which can directly and indirectly affect the soil enzyme activity. In the soil ecosystem, pH, TOC, TN and TP were important factors in counteracting damage to enzyme activity from FT effects and a suitable environment and adequate nutrients can limit damage to enzymes from FT events. The findings will better predictions the changing patterns of climate change on soil N-hydrolyzing enzyme activity.