The scavenging of atmospheric trace gases has been recognized as one of the lifestyle-defining capabilities of microorganisms in terrestrial polar ecosystems. Several metagenome-assembled genomes of as-yet-uncultivated methanotrophic bacteria, which consume atmospheric CH4 in these ecosystems, have been retrieved in cultivation-independent studies. In this study, we isolated and characterized a representative of these methanotrophs, strain D3K7, from a subarctic soil of northern Russia. Strain D3K7 grows on methane and methanol in a wide range of temperatures, between 5 and 30 degrees C. Weak growth was also observed on acetate. The presence of acetate in the culture medium stimulated growth at low CH4 concentrations (similar to 100 p.p.m.v.). The finished genome sequence of strain D3K7 is 4.15 Mb in size and contains about 3700 protein-encoding genes. According to the result of phylogenomic analysis, this bacterium forms a common clade with metagenome-assembled genomes obtained from the active layer of a permafrost thaw gradient in Stordalen Mire, Abisco, Sweden, and the mineral cryosol at Axel Heiberg Island in the Canadian High Arctic. This clade occupies a phylogenetic position in between characterized Methylocapsa methanotrophs and representatives of the as-yet-uncultivated upland soil cluster alpha (USC alpha). As shown by the global distribution analysis, D3K7-like methanotrophs are not restricted to polar habitats but inhabit peatlands and soils of various climatic zones.

The Polar Regions (PRs) are characterized by ice sheets, sea ice, glaciers, tundra, and other cryospheric landscapes and associated aboriginal cultural features. This primitive polar landscape is a huge contrast to the current human living environment and is a strong tourist attraction. Rapid environmental changes and the emergence of conflicts between tourism development and ecological protection, have affected sustainable development of polar tourism (PT). Polar high-latitude characteristics determine the vulnerability of their environment and the higher sensitivity of PT to climate change. This study comprehensively analyzed the status quo of PT development, systematically revealed mutual influence between environmental changes and tourism development, and proposed some adaptive measures to coordinate environment protection and tourism development.

The permafrost regions currently occupy about one quarter of the Earth's land area. Climate-change scenarios indicate that global warming will be amplified in the polar regions, and could lead to a large reduction in the geographic extent of permafrost. Development of natural resources, transportation networks, and human infrastructure in the high northern latitudes has been extensive during the second half of the twentieth century. In areas underlain by ice-rich permafrost, infrastructure could be damaged severely by thaw-induced settlement of the ground surface accompanying climate change. Permafrost near the current southern margin of its extent is degrading, and this process may involve a northward shift in the southern boundary of permafrost by hundreds of kilometers throughout much of northern North America and Eurasia. A long-term increase in summer temperatures in the high northern latitudes could also result in significant increases in the thickness of the seasonally thawed layer above permafrost, with negative impacts on human infrastructure located on ice-rich terrain. Experiments involving general circulation model scenarios of global climate change, a mathematical solution for the thickness of the active layer, and digital representations of permafrost distribution and ice content indicates potential for severe disruption of human infrastructure in the permafrost regions in response to anthropogenic climate change. A series of hazard zonation maps depicts generalized patterns of susceptibility to thaw subsidence. Areas of greatest hazard potential include coastlines on the Arctic Ocean and parts of Alaska, Canada, and Siberia in which substantial development has occurred in recent decades.