A cutting-edge smart nano-hybrid technology, offering potential benefits for plants, has recently been developed to address the pervasive issue of heavy metal pollution. This study explored the potential of this technology in mitigating chromium (Cr) stress in rapeseed using a nano-based system that integrates 100 mu M hydrogen sulphide (H2S) and 50 mu M manganese nanoparticles (Mn-NPs). This strategy reveals Cr-stress tolerance mechanisms through physiological assessments and transcriptome data analysis. The results demonstrated that Cr stress substantially inhibited rapeseed growth while increasing oxidative damage markers (MDA and ROS levels). Conversely, Mn-NP and H2S co-treatment significantly mitigated these effects, as shown by: (1) restored growth metrics, (2) improved photosynthetic performance and membrane integrity, (3) optimized Mn/H2S homeostasis, and (4) reduced tissue Cr accumulation. The reduction in Cr content was attributed to enhanced Cr-detoxification mechanisms, driven by the upregulation of enzymatic antioxidant activities, like superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase. Transcriptomic profiling revealed marked upregulation of genes involved in core metabolic processes, including photosynthetic pathways, carbon assimilation, secondary metabolite biosynthesis, inositol/phosphatidylinositol signalling systems, and stress-response networks. Under Cr stress, Mn-NP and H2S co-treated rapeseed plants displayed enhanced tolerance, highlighting the crucial role of these signalling agents in activating Cr-defence mechanisms. Our findings demonstrate that the integration of nanotechnology and gasotransmitter signalling molecule H2S presents a novel strategy for enhancing heavy metal tolerance and plant productivity in contaminated soils.

Brassica napus is a biennial crop that is widely used for biofuel, fodder, and oil. Current study indicates that B. napus has a great potential for growth in marginal soils polluted with heavy metals. Transcriptome profiling of putative genes associated with chromium (Cr) absorption, transport, and accumulation in B. napus was used to study the molecular mechanism of plant resistance to 50-mu M Cr stress. The results demonstrated significant reductions in morphological and physiological attributes, changes in related gene profiles, cell structural damage, and downregulation of photosynthesis-associated genes. Furthermore, the plants showed the ability to recover from Cr-induced damage by controlling Cr uptake and maintaining redox balance in photosynthesis under stressful conditions. Following the Cr treatment, plant roots absorbed high Cr and stored it in cell walls, to decrease the absorption to aboveground plant parts. Under Cr treatment, 2401 differentially expressed genes (DEGs) were identified. Cr-induced DEGs were related to photosynthesis, metal-ion chelation, and heavy-metal transport. Co-expression and weighted correlation network analysis revealed the relevance of ABC transporter pathways and glutathione metabolism in B. napus Cr tolerance. This study has the potential to provide a molecular and genetic basis for the validation of future candidate genes and the breeding of crops with similar properties.

AimsOpencast lignite mining causes significant disturbances to the natural environment. It isn't only the plant cover that is destroyed, also the soil cover is damaged. Soils are replaced by dumps with material composition that properties differ significantly from natural soils. Reclamation of these areas is necessary.MethodsThis study presents the effect of forty-three years of agricultural reclamation involving alternating winter wheat and winter rapeseed in three fertilization treatments: 0- (without fertilization), I-NPK and II-NPK on the chemical properties of Technosols.ResultsThe investigation demonstrated that the Ap-horizon emerged in the case of I-NPK and II-NPK treatments. There was an improvement in chemical properties for the Ap-horizon as compared to 1978: soil organic carbon (SOC), total nitrogen (TN), available phosphorus (P) and potassium (K) increased. The CaCO3 decreased, and SOC/TN ratio declined, while pH and cation exchange capacity (CEC) remained unchanged. For the Technosols' surface horizon of the 0-NPK, there were also temporal increases in TN and SOC with a decrease in the SOC/TN ratio, whereas P, K, pH, CEC and CaCO3 values did not change significantly.ConclusionIn the 43-year-old post-mining Technosols, under the effect of fertilization and cultivated plants, the Ap horizon has formed, while in the non-fertilized soil the AC and CA horizons. Soil that were fertilized had significantly higher SOC, TN, P and K values in the surface horizon than minesoils without fertilization. In the subsurface horizons, the properties of minesoils were similar regardless of fertilization.

In the context of sustainable materials, this study explores the effects of accelerated weathering testing and bacterial biodegradation on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)/rapeseed microfiber biocomposites. Accelerated weathering, simulating outdoor environmental conditions, and bacterial biodegradation, representing natural degradation processes in soil, were employed to investigate the changes in the mechanical, thermal and morphological properties of these materials during its post-production life cycle. Attention was paid to the assessment of the change of structural, mechanical and calorimetric properties of alkali and N-methylmorpholine N-oxide (NMMO)-treated rapeseed microfiber (RS)-reinforced plasticized PHBV composites before and after accelerated weathering. Results revealed that accelerated weathering led to an increase in stiffness, but a reduction in tensile strength and elongation at break, of the investigated PHBV biocomposites. Additionally, during accelerated weathering, the crystallinity of PHBV biocomposites increased, especially in the presence of RS, due to both the hydrolytic degradation of the polymer matrix and the nucleating effect of the filler. It has been observed that an increase in PHBV crystallinity, determined by DSC measurements, correlates with the intensity ratio I1225/1180 obtained from FTIR-ATR data. The treatment of RS microfibers increased the biodegradation capability of the developed PHBV composites, especially in the case of chemically untreated RS. All the developed PHBV composites demonstrated faster biodegradation in comparison to neat PHBV matrix.

This study aimed to investigate whether presoaking with hemin (5 mu mol center dot L-1) could alleviate NaCl stress during rapeseed seedlings' growth and its role in the regulation of photosynthesis. In this experiment, 'HUAYOUZA 62 (HYZ 62)' and 'HUAYOUZA 158R (158R)' were used as materials for pot experiments to study the morphology, photosynthetic characteristics, antioxidant activity, and osmoregulatory factors of seedlings under different salt concentrations, as well as the regulatory effects of hemin-presoaked seeds. Our findings revealed that, compared the control, NaCl stress inhibited the growth of two rapeseed varieties, decreased the seedling emergence rate, and increased the content of malondialdehyde (MDA), the electrolyte leakage rate (EL) and antioxidant enzyme activity. Hemin soaking alleviated the adverse effects of salt stress and increased plant height, root elongation and dry matter accumulation. Compared with all NaCl treatments, hemin significantly enhanced photosynthetic indexes, including a percent increase of 12.99-24.36% and 5.39-16.52% in net photosynthetic rate (Pn), 17.86-48.08% and 8.6-23.44% in stomatal conductivity (Gs), and 15.42-37.94% and 11.09-19.08% in transpiration rate (Tr) for HYZ62 and 158R, respectively. Moreover, hemin soaking also increased antioxidant enzyme activities, including superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), reducing the malondialdehyde, and thus resulting in the alleviation of oxidative damage caused by NaCl stress. Furthermore, hemin stimulated the formation of soluble protein, which effectively regulated the osmo-protective qualities. The current findings strongly elucidate that hemin soaking could effectively alleviate the negative impacts of NaCl stress by regulating the morphological, photosynthetic, and antioxidant traits. This study provides a new idea regarding the effect of Hemin on the salt tolerance of rapeseed, and provides a basis for the practical application of Hemin in saline-alkali soil to improve the salt tolerance of cultivated rapeseed.