PurposeThis study aims to investigate the effects of root exudates on the aggregate stability and permeability of loess and to further reveal the mechanisms of vegetation in preventing and controlling soil erosion beyond mechanical effects.Materials and methodsWetting tests were conducted to investigate how loess aggregate stability varies with curing time and root exudate concentration; and infiltration tests were carried out to examine the influence of root exudates on the infiltration characteristics of loess with varying degrees of compaction.Results and discussionThe results showed that the stability of loess aggregates significantly increased due to the application of root exudates. Curing could enhance the stabilizing effects of root exudates on loess aggregates; however, there existed a critical curing duration. The application of root exudates reduced the stable infiltration rate and hydraulic conductivity of loess. However, untreated specimens under lower degrees of compaction exhibited lower stable infiltration rate and hydraulic conductivity due to local structural damage. The stable infiltration rate of both treated and untreated specimens decreased with curing time.ConclusionsThe effects of root exudates can be attributed to their ability to function as stabilizing agents and promote aggregation, due to their high adsorption capacities and negatively charged groups on their surfaces. On the other hand, the presence of root exudates can significantly enhance the soil microbial activity, the microorganisms and their hyphae further strengthen the soil structure, fill pores and increase the soil hydrophobicity, thereby improving the aggregate stability while reducing the soil permeability.

The growth of different grafted guava was different as affected by grafting on different rootstock varieties, which also influenced the damage degree of Spodoptera litura larvae. The co-regulation of the pest gut by rhizosphere microorganisms and root exudates may contribute to this differential damage. In this study, the microorganisms of soil, plants, S. litura larvae and root exudates of guava grafted on different rootstock varieties were analysed and compared. The activities of superoxide dismutase, peroxidase and catalase in the midgut of S. litura larvae feeding on heterograft leaves of guava (where rootstock and scion are of the different variety) were significantly higher than those in the midgut of S. litura larvae feeding on homograft leaves of guava (where rootstock and scion are of the same variety), and glutathione s-transferase activity showed an opposite result. Enterococcus spp. and Escherichia spp. were the two bacterial genera with the greatest difference in abundance in the midgut of S. litura larvae and exhibited a negative correlation with each other. The root system of guava influenced the root structure, soil nutrients and the population structure and diversity of rhizosphere microorganisms by regulating the type and amount of root exudates. Root exudates also influenced the physiological and biochemical status of S. litura larvae by regulating the rhizosphere microorganisms driving the tritrophic interaction of plant-microbes-insects. Based on our results and the observed differences in pest occurrence among different grafted plants, improving varieties through grafting may become an effective strategy to reduce the impact of insect pests on guava.

Background and aims The changes in soil physical properties caused by root exudates depend largely on the chemical composition of root exudates. Our aim was to explore the effects of non-specific root exudates on the physical properties of soil change. Methods Five sugar compounds, five amino acid compounds, and five organic acid compounds were selected and added to loess as three single addition treatments (amino acids, organic acids, and sugars) and four combined addition treatments (amino acids + organic acids, amino acids + sugars, organic acids + sugars, and amino acids + organic acids + sugars). Soil water repellency, aggregate stability, and shear resistance tests were performed on the loess. Results The treatments sugars, amino acids, and amino acids + sugars significantly increased soil water repellency. In addition, organic acids + sugars maximised mean weight diameter (MWD), geometric mean diameter (GMD) and the content of > 0.25 mm water-stable aggregates (R0.25), and minimised the percentage of aggregates destroyed (PAD) in the addition treatments. All treatments except for amino acids significantly increased soil shear strength and cohesion of the loess. Amino acids, amino acids + sugars, and amino acids + organic acids + sugars significantly increased the internal friction angle. Conclusion The single addition treatments had a higher effect on soil hydraulic properties, while the combined addition treatments had a higher effect on soil mechanical properties. Sugars and amino acids substantially increased soil hydraulic stability. Sugars combined with other compounds, especially with organic acids, significantly improved soil mechanical stability.

Mucilage offers several beneficial functions for soils, yet its impact on soil mechanical behavior remains underexplored. This study investigated the effects of chia seed mucilage on the plasticity index (under normal conditions) and penetration resistance (under compaction) of sandy clay loam soils with differing soil organic carbon (SOC) levels from Akhtar Abad (SOC = 1.6%) and Najm Abad (SOC = 0.6%) in northern Iran. Four mucilage concentrations (0, 1 g kg-1, 3 g kg-1, 5 g kg-1) and three compaction pressures (100 kPa, 300 kPa, 600 kPa) were used. We found that mucilage significantly increased the plasticity index, with a 5.8% increase at 1 g kg-1 in the Najm Abad soil and 2.6%-3% increases at higher concentrations in the Akhtar Abad soil. At a concentration of 3 g kg-1, soil penetration resistance in the Akhtar Abad soil increased by 0.9 MPa and 1.6 MPa at compaction pressures of 300 kPa and 600 kPa, respectively. In the Najm Abad soil, a concentration of 5 g kg-1 led to increases of 0.7 MPa and 1.7 MPa at compaction pressures of 300 kPa and 600 kPa, respectively. No significant relationship was found between soil penetration resistance and soil plasticity index. The mucilage-induced increase in soil plasticity may hinder soil workability, especially when tillage occurs immediately after crop harvest. Mucilage can also increase soil resistance to root penetration in areas compacted by heavy machinery. To mitigate these risks, we recommend performing tillage and machinery operations in both agricultural and forest ecosystems during dry periods, when mucilage is less active, to minimize its negative impact on soil workability and compaction.

In the past few decades, Cadmium-contaminated soil in agricultural fields has been a major global issue. The wide attention followed in agricultural production and the remediation of cadmium pollution in soil by cotton plants, due to the characteristics of wide planting area, the large biomass, strong capacity of cadmium accumulation, and non-edible properties of fiber. But the root secretion mechanism of cotton plants in response to cadmium threat is still unclear. In this study, four CdCl2 concentrations (0, 150, 300,450 mu mol/L) were applied to the soil at seedling stage, and physiological indicators of cotton seedling were detected and root exudates were collected after 10 days of cadmium exposure. The results showed that the cadmium tolerance of cotton seedlings was activated to the greatest extent under 300 mu mol/L cadmium, and inhibited when the concentration reached 400 mu mol/L. A total of 407 metabolites were detected based on UPLC-MS/MS. The composition and content of root exudates of cotton seedlings were significantly changed by cadmium stress, and there were 7 common differential accumulated metabolites, including isomaltulose, quinic acid, citric acid, gamma-aminobutyric acid, isomaltulose, galactinol and gluconic acid. KEGG analysis showed that there were 7 metabolic pathways highly related to cadmium stress, including pyruvate metabolism, glyoxylate and dicarboxylate metabolism, citrate cycle, galactose metabolism, starch and sucrose metabolism, ABC transporters and carbon metabolism. These metabolic pathways were involved in osmoregulation, energy supply and resilience in plants. In addition, exogenous addition of citric acid can enhance the antioxidant capacity of cotton leaves, and promote the absorption and accumulation of cadmium in cotton. This study provides a theoretical basis for further research on elucidating the response mechanism of root exudates in cotton plants to cadmium stress and for utilizing root exudates such as citric acid to alleviate cadmium stress.

Escalating anthropogenic activities have caused heavy metal contamination in the environmental matrices. Due to their recalcitrant and toxic nature, their occurrence in high titers in the environment can threaten survival of biotic components. To take the edge off, remediation of metal-contaminated sites by phytoremediators that exhibit a potential to withstand heavy metal stress and quench harmful metals is considered an eco-sustainable approach. Despite the enormous potential, phytoremediation technique suffers a setback owing to high metal concentrations, occurrence of multiple pollutants, low plant biomass, and soil physicochemical status that affect plants at cellular and molecular levels, inducing morphological, physiological, and genetic alterations. Nevertheless, augmentation of soil with microorganisms can alleviate the challenge. A positive nexus between microbes, particularly plant growth-promoting microorganisms (PGPMs), and phytoremediators can prevent phytotoxicity and augment phytoremediation by employing strategies such as production of secondary metabolites, solubilization of phosphate, and synthesis of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase and phytohormones. Microbes can mediate tolerance in plants by fortifying their antioxidant machinery, which maintains redox homeostasis and alleviates metal-induced oxidative damage in the plants. Associated microbes can also activate stress-responsive genes in plants and abridge metal-induced toxic effects. An in-depth exploration of the mechanisms employed by plant-associated microbes to trigger tolerance in phytoremediators is crucial for improving their phytoremediation potential and real-world applications. The present article attempts to comprehensively review these mechanisms that eventually facilitate the development of improved/new technology for soil ecosystem restoration.

Root-knot nematodes (Meloidogyne spp.) have garnered significant attention from researchers owing to the substantial damage they cause to crops and their worldwide distribution. However, controlling these nematodes is challenging because a limited number of chemical pesticides and biocontrol agents are effective against them. Here, we demonstrate that pepper rotation markedly reduces Meloidogyne incognita infection in cucumber and diminishes the presence of p-hydroxybenzoic acid in the soil, a compound known to exacerbate M. incognita infection. Pepper rotation also restructures the rhizobacterial community, leading to the colonization of the cucumber rhizosphere by two Pseudarthrobacter oxydans strains (RH60 and RH97), facilitated by enrichment of palmitic acid in pepper root exudates. Both strains exhibit high nematocidal activity against M. incognita and have the ability to biosynthesize indoleacetic acid and biodegrade p-hydroxybenzoic acid. RH60 and RH97 also induce systemic resistance in cucumber plants and promote their growth. These data suggest that the pepper root exudate palmitic acid alleviates M. incognita infection by recruiting beneficial P. oxydans to the cucumber rhizosphere. Our analyses identify a novel chemical component in root exudates and reveal its pivotal role in crop rotation for disease control, providing intriguing insights into the keystone function of root exudates in plant protection against root-knot nematode infection.

Nano polystyrene (PS) particles and antibiotics universally co-exist, posing a threat to crop plants and hence human health, nevertheless, there is limited research on their combined toxic effects along with major influential factors, especially root exudates, on crop plants. This study aimed to investigate the response of Chrysanthemum coronarium L. to the co-pollution of nanoplastics and tetracycline (TC), as well as the effect of root exudates on this response. Based on a hydroponic experiment, the biochemical and physiological indices of Chrysanthemum coronarium L. were measured after 7 days of exposure. Results revealed that the co-pollution of TC and PS caused significant oxidative damage to the plants, resulting in reduced biomass. Amongst the two contaminants, TC played a more prominent role. PS could enter the root tissue, and the uptake of TC and PS by plant roots was synergetic. Malic acid, oxalic acid, and formic acid could explain 65.1% of the variation in biochemical parameters and biomass of the roots. These compounds affected the photosynthesis and biomass of Chrysanthemum coronarium L. by gradually lowering root reactive oxygen species (ROS) and leaf ROS. In contrast, the impact of rhizobacteria on the toxic response of the plants was relatively minor. These findings suggested that root exudates could alleviate the toxic response of plants to the co-pollution of TC and PS. This study enhances our understanding of the role of root exudates, providing insights for agricultural management and ensuring food safety.

Microplastics (MPs) are important carriers of various toxic metals and can alter their toxicity pattern in agricultural soil, leading to combined pollution, therefore posing new challenges to soil pollution management and environmental risk assessment. In this study, we observed the internalization of MPs in plants and conducted incubation experiments to evaluated the effects of arsenate (As(V)) alone and in combination with polystyrene (PS) MPs on wheat seedlings (Triticum aestivum L.). Under As(V) alone and combined with PS-MP exposure, dosedependent toxicity in terms of root and stem elongation and biomass accumulation was observed. Compared with As(V) alone, the presence of PS-MPs reduced the accumulation of As in wheat roots by 11.43-58.91%, but PSMPs intensified the transport of As to the aboveground parts of wheat, increasing As accumulation in wheat stems by 27.77-1011.54%. This causes more serious mechanical damage and oxidative stress to plant cells, increasing the accumulation of reactive oxygen species and lipid peroxidation in wheat roots and upregulating the activities of antioxidant enzymes such as superoxide dismutase (SOD) and peroxidase (POD). In addition, the co-exposure of As(V) and PS-MPs disrupts the photosynthetic system of wheat leaves and the secretion activities of roots. Therefore, the combination of As(V) and PS-MPs caused greater damage to wheat growth. Our findings contribute to a more comprehensive assessment of the combined toxicity of MPs and heavy metal to crops.

Plants exert a profound influence on their rhizosphere microbiome through the secretion of root exudates, thereby imparting critical effects on their growth and overall health. The results unveil that japonica rice showcases a remarkable augmentation in its antioxidative stress mechanisms under Cd stress. This augmentation is characterized by the sequestration of heavy metal ions within the root system and the prodigious secretion of a spectrum of flavonoids, including Quercetin, Luteolin, Apigenin, Kaempferide, and Sakuranetin. These flavonoids operate as formidable guardians, shielding the plant from oxidative damage instigated by Cd-induced stress. Furthermore, the metagenomic analyses divulge the transformative potential of flavonoids, as they induce profound alterations in the composition and structural dynamics of plant rhizosphere microbial communities. These alterations manifest through the recruitment of plant growth-promoting bacteria, effectively engineering a conducive milieu for japonica rice. In addition, our symbiotic network analysis discerns that flavonoid compounds significantly improved the positive correlations among dominant species within the rhizosphere of japonica rice. This, in turn, bolsters the stability and intricacy of the microenvironmental ecological network. KEGG functional analyses reveal a notable upregulation in the expression of flavonoid functional genes, specifically cadA, cznA, nccC, and czrB, alongside an array of transporters, encompassing RND, ABC, MIT, and P-ATPase. These molecular orchestrations distinctly demarcated the rhizosphere microbiome of japonica rice, markedly enhancing its tolerance to Cd-induced stress. These findings not only shed light on the establishment of Cd-resistant bacterial consortia in rice but also herald a promising avenue for the precise modulation of plant rhizosphere microbiomes, thereby fortifying the safety and efficiency of crop production.