Intervertebral disc degeneration (IVDD) is a globally prevalent disease, yet achieving dual repair of tissue and function presents significant challenges. Considering reactive oxygen species (ROS) is a primary cause of IVDD, and given the decrease of nucleus pulposus cells (NPCs) and extensive degradation of extracellular matrix (ECM) during IVDD development, the present study, inspired by the seeds-and-soil strategy, has developed NPCsloaded TBA@Gel&Chs hydrogel microspheres. These microspheres serve as exogenous supplements of NPCs and ECM analogs, replenishing seeds and soil for nucleus pulposus repair, and incorporating polyphenol antioxidant components to interrupt the oxidative stress-IVDD cycle, thereby constructing a microsphere system where NPCs and ECM support each other. Experiments proved that TBA@Gel&Chs exhibited significant extra-cellular ROS-scavenging antioxidant capabilities while effectively upregulating intracellular antioxidant proteins expression (Sirt3 and Sod2). This dual-action antioxidant capability effectively protects the vitality and physiological functions of NPCs. The therapeutic effects of microspheres on IVDD were also confirmed in rat models, which was found significantly restore histological structure and mechanical properties of degenerated discs. Additionally, RNA-seq results have provided evidences of antioxidant mechanism by which TBA@Gel&Chs protected NPCs from oxidative stress. Therefore, the NPCs-loaded TBA@Gel&Chs microspheres developed in this study have achieved excellent therapeutic effects, offering a paradigm using antioxidant biomaterials combined with cellular therapy for IVDD treatment.

Enhancing the catalytic activity of inorganic minerals is crucial for advancing wastewater treatment technologies. In this study, carbon and lab-scale minerals were combined to develop a novel carbon-based material, termed paired mineral carbon (PMC), using rice husk (RH) as the carbon source. Montmorillonite (MMt), goethite (GTt), and hybrid of goethite and MMt were utilized to prepare the PMC. The resulting material exhibited an increased specific surface area of 187 m2g-1, and demonstrated exceptional activation efficiency of peroxymonosulfate (PMS) for degrading diethyl phthalate (DEP). A pseudo-first-order kinetic constant (k1) of 0.923 min-1 was achieved at pH 6.0. Pairing minerals synergistically altered PMC's structure, which had the highest ID/IG ratio (0.87) indicative of abundant defective sites in its hierarchical porous structure. Reactive species such as SO4 center dot-, center dot OH, and 1O2 were identified as key contributors to DEP degradation through electron spin resonance (ESR) and quencher experiment. Density functional theory (DFT) calculations further revealed preferential radical attack on DEP at specific atomic sites (f0 values: 0.0837-0.1027). Furthermore, the lab-scale synthesized PMC costs $8.08 kg-1. More versatile than commercial activated carbon ($10 kg-1). The simple, adaptive, scalable synthesis optimizes industrial costs. Moreover, phytotoxicity assessment demonstrated that PMC/PMS treatment significantly reduced DEP toxicity, promoting healthier growth of Raphanus sativus and Zea mays seedlings. These findings highlight the potential of PMC as an eco-friendly, efficient and economical catalyst for advanced wastewater treatment, offering a sustainable approach to managing both chemical pollutant and herbicide phytotoxicity.

The neotropical palm Mauritia flexuosa produces seeds that show the association between recalcitrance and dormancy. Despite the intolerance to desiccation, the seeds can maintain persistent banks in flooded environment soils (veredas) in the Cerrado biome. As the mechanisms involved in the persistence of recalcitrant seed banks are still poorly understood, the objective of this work was to evaluate the response of M. flexuosa embryos to water deficit and saturation stresses. Embryos of M. flexuosa with water content typical of dispersion or subjected to hydration were exposed to moderate and severe water potentials (Psi w= -1.5 MPa and Psi w= -2.1 MPa), in addition to water saturation (Psi w= 0 MPa). Anatomical, histochemical and ultrastructural evaluations were performed on the embryos after 24 h. Membrane integrity estimation, endo-beta-mannanase activity and oxidative stress indicators (H2O2 and MDA contents, CAT, SOD and APX activity) were also evaluated. The endosperm structure contributes to the maintenance of embryo hydration, while abundant mucilage reserves favor resilience to desiccation. Post-dispersal hydration makes embryos less vulnerable to oxidative stress, which is due to the non-enzymatic antioxidant system. Both moderate water stress and post-dispersal water absorption induce an increase in metabolism and the mobilization of reserves, which indicate that hydration/dehydration cycles can favor overcoming dormancy. M. flexuosa embryos show resilience to water deficit, and that is crucial for the persistence of seeds in the soil in seasonal environments, however, successful germination is dependent on high hydration, which prevents structural and physiological damage.

Most crop species are cultivated in nutrient-deficient soils, in combination with other challenging constraints that are exacerbated by the current climate changes. The significance of micronutrient shortage in stress management is often underappreciated, although their deficiency restricts both plant growth and resistance to abiotic stresses and diseases. While the application of nutrients to growing plants is a potential strategy to improve plant resistance to abiotic stresses, seed nutrient status may also play a role in crop stress tolerance as a storage and accumulation site of nutrients. To avoid hidden hunger problems, developing countries need to increase domestic cereal production, enhance their resilience to extreme weather events, and improve their nutritional status and quality. Here, we analyze the accumulated knowledge about the effects of nutri-priming in cereal crop species with a focus on mechanisms of application and stress tolerance, keeping in mind the risk of crop damage mostly caused by global climate change, which is driving an alarming increase in the frequency and intensity of abiotic stresses. We also propose new approaches to food production, which may be promising solutions for global warming, emerging diseases, and geopolitical conflicts recognized as major drivers of food insecurity.

The population dynamics of stem-borer weevil, Ceutorhynchus spp. (Col.: Curculionidae) was studied in canola under field conditions in some parts of Iran during 2016-2018. Larvae cause damage by feeding inside petioles, crowns and/or stems of the crop. Meanwhile, the biology and distribution of the pest have not been previously investigated in the canola fields of Iran. In the present research, the pest adult samples were collected from three 1-hectare fields in Mazandaran, Golestan and Lorestan provinces by yellow water traps at weekly intervals during crop growing season. For collecting weevil-infested crops and determining infestation rate, 10 quadrates (0.5 x 0.5 m) were used per field. The adult weevils collected by the traps were identified and the pest biology as well as the infestation rates of the crop by the weevils were studied. The results showed that in late November, the adult weevils migrated to canola fields and after feeding and mating, the females deposited their eggs into main veins, petioles of the lower leaves or inside the collar area of young canola plants and the emerged larvae penetrated the host plant. The three larval instars of this pest fed inside host plant tissue during autumn and winter until the mid-spring season. In late April, third instar larvae left the host plant, dropped on the soil for pupation and new generation adults emerged in May. The maximum infestation rates of oilseed rape by the stem weevil in Mazandaran, Lorestan and Golestan provinces were 20.1 +/- 1.4, 14.8 +/- 0.99 and 10.2 +/- 0.79%, respectively. In this study, flight and oviposition activity of adults and feeding activity of the first instar larvae were mainly observed in late November, which suggests this time is a suitable time for control measures, if necessary. As an integral part of pest management strategy in oilseed rape fields, determining the time of adult migration activity, oviposition and larval development of the weevil is necessary.

The issue of storage and protection of yilded seeds from storage pests is fundamental to seed science, the nutrition of people and animals as well as the maintenance of state reserves. During 2019-2021, in the experimental field of IASS Obraztsov chiflik - Rousse, in a long-term stationary fertilizer experiment, seeds of common bean variety Obraztsov chiflik 12 were obtained utilizing the following fertilization options: 1 - individual nitrogen fertilization; 2 application; 6 - combined PK application; 7 - triple combination of NPK. An unfertilized control variant was maintained for comparison purposes. The study aims to entomologically evaluate seeds regarding damage by bean weevils after crop harvesting. Measurements of yield, 1000-seed weight, damaged seeds percentage, Index of infestation, as well as weight loss were taken. The combined application of NP demonstrated the greatest positive effect on the yield qualities of beans, however it lead to an intermediate position of the yield in terms of damage by bean weevil. The variant treated with combined NK fertilization resulted in the lowest percentage of damaged seeds and Index of infestation; it was followed by the variant with full mineral fertilization and the control variant. The individual application of N resulted in highest damaged seeds percentage as well as highest Index of infestation; it also ensured the highest weight loss on average for the studied period.