Vegetable production on plastic mulch in Georgia often combines fumigation, drip tape, raised beds, and plastic mulch, where three to five high-value crops are produced over 2 yr. With the elimination of methyl bromide as a soil fumigant, herbicides applied over plastic mulch before crop transplanting have become essential to maintain weed control. However, proper care must be taken to avoid crop damage from any herbicide residue. Experiments using simulated vegetable beds covered with totally impermeable film (TIF) were conducted to quantify the concentration of halosulfuron-methyl, glufosinate, glyphosate, S-metolachlor, and acetochlor remaining on the mulch and the amount of each herbicide that moved into the crop transplant hole when irrigation water was applied. With 0.63 cm of water irrigation, glufosinate > halosulfuron-methyl > S-metolachlor > acetochlor. All herbicide concentrations were below 1.0 mg ai/ae in the transplant hole regardless of irrigation volume. For halosulfuron, glyphosate, and glufosinate, these concentrations were equal to a 1.3 to 8.9 times the field use rate washing into the transplant hole. Acetochlor and S-metolachlor concentrations in the transplant hole were equivalent to 0.1x to 0.7x of field use rates, respectively. With further evaluations, the quantified herbicide concentrations in the transplant hole can be used to make changes to recommended rates and potentially create new options for growers to utilize.