Atrazine (ATR) is a widely utilized herbicide that has been demonstrated to exert a multitude of deleterious effects on the environment, particularly with regard to water and soil contamination. Moreover, its disruption of endocrine function and implications for antibiotic resistance underscore the urgent need to prioritize alternative solutions for both ecosystems and human health. Therefore, the objective of this study was to investigate a range of neurotoxic effects associated with atrazine-induced damage in the prefrontal lobe of mice. The results of this study indicate that treatment with ATR in C57BL/6 J mice resulted in cognitive-related behavioral deficits, including anxiety and depression, as well as motor impairments. In vivo analyses demonstrated that ATR exposure resulted in a reduction in neuronal synapse density at the microstructural level, while also compromising prefrontal morphological integrity, nociceptor count, and overall neuronal health within the brain. These findings collectively suggest that synaptic deficits are implicated in ATR-induced behavioral abnormalities observed in these mice. Furthermore, our findings revealed that ATR exposure resulted in elevated TDP-43 expression levels that were ectopically localized within the cytoplasm. This alteration led to impaired functionality of mRNP granules and contributed to the development of abnormal synaptic defects. Conversely, TDP43 has the potential to localize ectopically to mitochondria, where it activates the mitochondrial unfolded protein response (UPRmt), which ultimately results in mitochondrial dysfunction. These findings collectively indicate a strong correlation between TDP-43 dysregulation and the progression of neurodegenerative diseases. Further investigation into the potential neurotoxicity of atrazine may foster heightened awareness, leading to more stringent regulatory measures, research into safer alternatives, and the adoption of sustainable practices, which are essential for safeguarding environmental integrity alongside human health.

The delayed breakage of particles significantly affects the long-term mechanical properties of rockfill materials. This study examines the effects of particle strength dispersion on the distribution of time-dependent strength using fracture mechanics and probabilistic methods. Subsequently, the distribution of normalized maximum contact force (NMCF), defined as the ratio of the maximum contact force to instantaneous strength, for specimens with uniform particle size is derived using extreme value theory and Discrete Element Method (DEM). Based on this analysis, the probabilities of delayed breakage in rockfill specimens over various time intervals are calculated using a joint probability delayed breakage criterion. The feasibility of the proposed method is validated by comparing theoretical calculation with DEM triaxial creep simulation results that accounted for particle breakage. The findings offer innovative tools and theoretical insights for understanding and predicting the particle delayed breakage behavior of rockfill materials and for developing macro-micro creep crushing constitutive models.

The K & uuml;& ccedil;& uuml;k & ccedil;ekmece-Avc & imath;lar corridor of the D100 highway constitutes a critical component of Istanbul's transportation infrastructure. Given its strategic importance, ensuring its operational continuity following the anticipated major Istanbul earthquake is imperative. The aim of this study was to investigate the liquefaction-induced geotechnical risks threatening the K & uuml;& ccedil;& uuml;k & ccedil;ekmece-Avc & imath;lar segment of the D100 highway. Initially, the study area's liquefaction susceptibility was assessed through Liquefaction Potential Index mapping. Subsequently, post-liquefaction ground displacements were quantified using semi-empirical methodologies and advanced numerical analyses focused on representative critical sections. Numerical simulations incorporated various constitutive models for liquefiable soils, enabling a comparative assessment against semi-empirical estimations. The results revealed that semi-empirical approaches systematically overestimated the lateral displacements relative to numerical predictions. Moreover, the analyses highlighted the sensitivity of model outcomes to the selection of constitutive parameters, underscoring the necessity for careful calibration in modeling liquefiable layers. Despite considering the most conservative displacement values from numerical analyses, findings indicated that the D100 highway is likely to experience substantial damage, potentially leading to extended service disruptions following the projected seismic event.

Durum wheat cultivation is increasingly threatened by viral diseases worldwide. Soil-borne cereal mosaic virus (SBCMV) and wheat spindle streak mosaic virus (WSSMV) cause significant crop losses in Europe. These viruses are transmitted through a soil-inhabiting vector, the plasmodiophoromycota Polymyxa graminis Led. There are very few methods available to eradicate P. graminis, whose resting spores survive in infested soil for decades, but they are either too expensive or not environmentally friendly. Therefore, it is crucial to develop resistant wheat varieties to mitigate the damage. For this purpose, more than 200 durum wheat genotypes, mostly landraces, were selected from the Global Durum Wheat Panel germplasm collection. Then, an experiment was conducted in a semi-controlled environment: the genotypes were sown in pots containing soil infested by P. graminis carrying SBCMV and WSSMV and maintained through the winter period. In early spring, visual assessment of viral symptomatology was performed. Subsequently, the viral loads of the two viruses in leaf tissues were determined through qRT-PCR analysis. The tested genotypes exhibited different responses to the two viruses: SBCMV showed very diversified viral loads among genotypes, whereas WSSMV infected all genotypes. We identified 23 genotypes, with low viral loads of both viruses and reduced symptoms, that could be of particular interest for breeders aiming at new resistant durum wheat varieties. A pilot GWAS allowed to identify genomic regions putatively associated to resistance to SBCMV or WSSMV, as well as possible candidate genes involved in these traits.

The overapplication of chemical pesticides will cause heavy pollution in water, soil, and foodstuff, and cause irreversible damage to the ecological environment and human health. Therefore, it is imperative to develop a highly sensitive and reliable tool for detecting pesticide residues in the environment. In this work, a novel nopinone-based fluorescent probe THIP-OCP for the detection of parathion-methyl was constructed from BchE inhibition principles. The ester bond in THIP-OCP was hydrolyzed by BchE, leading to the release of the fluorophore THIP-OH and a significant enhancement of the fluorescence signal at 547 nm. However, parathion- methyl could inhibit BchE activity significantly and resulted in fluorescence quenching at 547 nm. Probe THIP-OCP was effectively used to detect BchE and parathion-methyl, and the detection limits were as low as 8.56 U/L and 0.79 mu g/mL, respectively. A portable smartphone-based analysis platform for quantitative and qualitative analysis of parathion-methyl in soil was developed from probe THIP-OCP. This probe can also be used to detect butyrylcholinesterase (BchE) and parathion-methyl in living cells and zebrafish, providing a new tool for monitoring BchE and parathion-methyl in living systems, which is helpful for protecting human life and health. Therefore, the probe THIP-OCP is regarded as a promising tool for monitoring environmental safety and biological health systems.

Hordeum jubatum L. is a perennial herb with high ornamental value and strong stress tolerance. Nitrogen deposition and cold stress are key environmental factors that affect stability of ecosystems in cold regions of northeast China. These factors significantly affect plant growth and development. Arbuscular mycorrhizal fungi (AMF) are symbiotic soil fungi that can increase plant resistance and growth. However, research on impacts of nitrogen deposition and cold stress on roots of H. jubatum-AM symbionts remains limited. Root biomass (dry and fresh weight), architecture (length, surface area, volume, forks, number of fourth-order roots, and root fractal dimension), and ultrastructure of H. jubatum were assessed, both in the presence and absence of AMF, under conditions of nitrogen deposition and cold stress. Cold stress inhibited all indicators of root architecture and disrupted root ultrastructure, with greater inhibition shown in the N2 (NH4+/NO3- = 1:1) treatment under cold stress, indicating nitrogen deposition increased sensitivity of H. jubatum to cold stress. Inoculation with AMF significantly reduced damage caused by nitrogen deposition and cold stress on H. jubatum roots compared with the non-inoculation treatment. Our results demonstrate different effects of the interaction of nitrogen deposition and cold stress versus single stress (nitrogen deposition or cold stress) on plant root development and provide a scientific basis for the use of mycorrhizal technology to improve resistance and productivity of cold-tolerant plants in cold regions under stress conditions.

Forest soil is crucial in climate change mitigation, food security, and biogeochemical nutrient cycling. Mixed Sal forests enhance soil organic matter, improve nutrient availability, and regulate pH dynamics. However, anthropogenic disturbances, including deforestation and land-use changes, significantly alter forest cover, leading to shifts in soil physicochemical and microbial properties. These impacts necessitate rigorous monitoring and comprehensive assessment. Therefore, we investigated the effects of contrasting conditions- closed (no human activities) and open (human interferences) mixed Sal Forest on the vertical and seasonal dynamics of microbial biomass carbon (SMBC). Results revealed that the closed mixed Sal Forest had significantly higher SMBC than the open mixed Sal Forest across the soil profile (D1-D5) with a strong seasonal effect. Closed mixed Sal Forest had 60% higher SMBC in D1 than open mixed Sal Forest while it reduced with depth and 17.1 to 56.7% higher SMBC in the subsurface to bottom-most soil profile (D2-D5). Moreover, SMBC was higher in the monsoon period in both forests. The SMBC reduced by 24.2 to 45.1% in the post-monsoon period while reduction was more intense in the pre-monsoon period (48.1 to 68.2%) compared to the monsoon period under closed mixed Sal Forest. Similarly, the decline was more intense in the open mixed Sal Forest, where SMBC declined 12.1 to 54% in the post-monsoon period and 56.1 to 76.2% in the pre-monsoon period compared to the monsoon period. The study indicates that human interference in mixed Sal forests leads to loss of forest cover, negatively affecting microbiological properties and reducing soil fertility, which weakens the forest's resilience to climate change. Additionally, SMBC exhibits seasonal variations, reflecting responses to environmental conditions. These results underline the need to reduce human disturbances and enhance forest conservation strategies to ensure soil sustainability and ecosystem stability.

Iron (Fe) is an essential trace element for plant growth, but its availability in saline soils is limited. Salt stress aggravates Fe-deficiency stress in plants. We report the effects of adding sodium nitroprusside (SNP) on peanut seedlings in saline and Fe-deficient environments. Seedlings were grown hydroponically. Seedling growth was compared after adding SNP to nutrient solutions: salt (100 mM NaCl), Fe (0 mu mol L- 1 EDTA-Fe), and combined Fe + salt (Fe + 100 mM NaCl). Combined salt and Fe-deficiency yellowed peanut leaves, caused severe oxidative stress, and inhibited seedling growth. Addition of SNP alleviated this damage, with each seedling height, fresh dry weight, and root viability increasing. Adding SNP promoted reduction in Fe3+, the transport of Fe from underground to aboveground seedling parts, and from the cell wall to organelles and soluble parts. SNP increased contents of active Fe and chlorophyll in leaves, alleviated new-leaf yellowing, increased antioxidant enzyme activities and osmotic regulator contents, and removed excess reactive oxygen species, and MDA content and electrolyte extravasation rates in leaves and roots, thereby maintaining membrane structure stability. SNP promoted absorption of K, Ca, and Mg and their transport to shoots, increased Fe availability, and improved the ion imbalance and toxicity caused by salt and Fe stress. Salt stress worsened Fe deficiency stress in peanut, but adding SNP alleviated this. SNP promoted the reduction and transport of Fe in peanut, and increased Fe availability, improving the reduction and absorption of Fe in the environmental medium by roots.

Carbonaceous aerosol components (CACs) significantly influence global radiative forcing and human health. We developed a simultaneous inversion algorithm for four CACs: black carbon (BC), brown carbon (BrC), watersoluble organic matter (WSOM), and water-insoluble organic matter (WIOM), considering their distinct optical, solubility, and hygroscopicity properties. Using AERONET data, we inverted the global concentrations of these components for 2022. We observed that the mass concentration of black carbon (BC) is highest in the South Asian region, with an annual average of 4.74 mg m(-2). High values of brown carbon (BrC) correspond well with regions and seasons of biomass burning, with the annual average reaching 9.03 mg m(-2) at sites in Central and West Africa. Water-insoluble organic matter (WIOM) is the most predominant component in carbonaceous aerosols, with an annual average concentration as high as 53.11 mg m(-2) at the Dhaka_University site in Eastern South Asia. Additionally, the study also points out a significant correlation between the dominant components of carbonaceous aerosols and their seasonal variations with local emissions. Furthermore, the validation of optical parameters against official AERONET products demonstrates a good correlation.

Bio-cement is a green and energy-saving building material, which has received wide attention in the field of ecological environment and geotechnical engineering in recent years. The aim of this study is to investigate the improvement effect of plant-based bio-cement (PBBC) in synergistic treatment of sand with organic materials, to highlight the effective use of tap water in PBBC, and to analyze the crack evolution pattern during the damage of specimens by using image processing techniques. The results showed that tap water can be used as a solvent for PBBC instead of deionized water. The characteristic trend of urease solutions prepared at different temperature environments was obvious, and the activity value of urease solution with low concentration is positively correlated with the ambient temperature, although the activity value is not high, it is not easy to inactivate. The incorporation of organic materials increased the peak stress up to 1809.30 kPa compared to the specimens modified only by PBBC. The damage of the specimens under uniaxial compression consisted of four stages: compaction, elastic deformation, pre-peak brittle damage and post-peak macroscopic damage. The corresponding crack evolution is the interpenetration of small-sized cracks into large-sized main cracks. The large-sized main cracks transform into penetration cracks before damage, and the small-sized cracks are distributed around the penetration cracks. The crack evolution parameters obtained by MATLAB processing are positively correlated with the strain.