We assessed the potentially pathogenic fungi present in Antarctic permafrost and the overlying active layer on King George, Robert, Livingston and Deception Islands in the South Shetland Islands archipelago, maritime Antarctica. Permafrost and active layer sub-samples were incubated at 37 & DEG;C to select fungi able to grow inside the human body. A total of 67 fungal isolates were obtained, 27 from the permafrost and 40 from the active layer. These represented 18 taxa of the genera Alternaria, Aspergillus, Curvularia, Penicillium, Rhodotorula and Talaromyces. The majority of fungi detected occurred exclusively either in the permafrost or the active layer at each site. Only Aspergillus thermomutatus, Penicillium cf. chrysogenum and Rhodotorula cf. mucilaginosa were present in both permafrost and active layer samples from the same site. The yeast R. cf. mucilaginosa was recovered from both in at least two sites. The genus Penicillium was the most abundant and widely distributed genus in both permafrost and active layer samples across the sites sampled. All fungal isolates were screened using enzymatic, pH and antifungal assays to identify their virulence potential. Aspergillus hiratsukae, A. thermomutatus and R. cf. mucilaginosa, known human opportunistic fungi, were identified, displayed phospholipase, esterase, proteinase and hemolytic activities. All three also displayed the ability to grow at 40 & DEG;, 45 & DEG; and/or 50 & DEG;C and resistance to fluconazole and itraconazole; additionally, R. cf. mucilaginosa showed resistance to amphotericin B and viability after 100 d at -80 & DEG;C. A. thermomutatus UFMGCB 17415 killed the entire larvae of Tenebrio molitor in six days and R. cf. mucilaginosa UFMGCB 17448 and 17473 in three and four days, respectively. The melting of maritime Antarctic permafrost as a result of climate change may threaten the release of wild strains of pathogenic fungi geographically isolated for long time, which may in turn be transported within and beyond Antarctica by different biological and non-biological vectors. (c) 2022 British Mycological Society. Published by Elsevier Ltd. All rights reserved.

Spring, especially the freeze-thaw season, is considered the key period for the growth and carbon sequestration of desert mosses. It is not clear how the change in environment water and temperature affects the physiological characteristics of desert mosses in freeze-thaw season. In this study, the effects of water and freeze-thaw cycles on the physiological characteristics of Syntrichia caninervis were assessed by manipulating the increase or removal of 65% snow and changes in the freeze-thaw cycles. The results showed that the changes in snow depth, freeze-thaw cycles, and their interaction significantly affected the plant water content, osmoregulatory substances content, antioxidant substance, and antioxidant enzyme activities. The contents of free proline, soluble sugar, ascorbic acid (AsA), reduced glutathione (GSH), and malondialdehyde (MDA), and superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities increased significantly with the decrease in snow depth and freeze-thaw cycles. POD and free proline were the most sensitive to the snow depth and freeze-thaw cycles, while SOD and CAT were the least sensitive. Therefore, compared with the increase in freeze-thaw cycles, the reduction in freeze-thaw cycles weakened the physiological sensitivity of S. caninervis to snow depth changes.

Thaw and liquid precipitation retard cooling of snow cover and soil surface and so may be a factor of heating. This slows down the soil freezing due to more active freezing of the wet snow, and, thus, promotes cooling and re-cooling of the soil. However, there are a number of factors which intensify the soil freezing after thaw. With thaw, the thickness of the snow cover decreases, and its density increases. In addition, after freezing wet snow improves the contact between the ice crystals, which increases the hardness and thermal conductivity of the snow As a result, after the thaw, the thermal protection ability of the snow decreases, and this can accelerate freezing of the soil. The dynamics of snow accumulation in Russia is considered in the paper. Using data obtained in the Western Svalbard, we demonstrate the increase in the number of thaws and liquid precipitation and influence of them on the snow cover and soil freezing. The influence of thaw on the growth of thermal resistance of snow cover is also considered. Calculations have shown that in the absence of a thaw, the depth of soil freezing is 1.26 m. With a thaw lasting 10 days, which begins on the 40th day from the start of soil freezing, the depth of freezing is reduced down to 1.2 m without considering changes in snow cover. When taking into account changes in the thermal resistance of snow cover, the depth of soil freezing by the end of the cold period increases up to 1.32 cm. With a thaw in the mid-winter, i.e. on the 70th day, the depth of freezing decreases down to 1.22 m, that is smaller than the depth of freezing without thaw This scenario is in accordance with changes in snow accumulation dynamics under the present-day climate, as in many areas most of the solid precipitation falls in the first half of the cold period. As a result, for a period after a thaw the smaller volume of snow will be deposited, and this will retard increasing in thermal resistance of the snow cover.

The characteristics of temperature dynamics in a solonetzic meadow-chernozemic soil of alas depression in Central Yakutia are described on the basis of long-term (2005-2014) stationary studies. Quick changes in weather conditions accompanied by changes in the soil temperature regime were observed during that period. Thus, the beginning of soil thawing in the spring shifted to earlier dates, and the beginning of soil freezing in the fall shifted to later dates. Temperature trends demonstrate an increase in the mean annual soil temperatures at all the depths. In total, the period of the frozen state of the soil became considerably shorter: in the middle-profile horizons, by 30-39 days. The obtained results attest to the high dynamism in temperature parameters of meadow soils in alas depressions of Central Yakutia under conditions of global climate changes.

Many-year variations of river runoff in the Selenga basin are analyzed along with precipitation, potential evapotranspiration, and basin water storages. Data of ground-based (1932-2015) and satellite observations, as well as the analysis of literature data suggest the presence of within-century cycles in the series of annual and minimum runoff. Compared with 1934-1975, the Selenga Basin shows a general tendency toward a decrease in the maximum (by 5-35%) and mean annual (up to 15%) runoff at an increase in the minimum runoff (by 30%), a decrease in the mean annual precipitation (by 12%), and an increase in potential evapotranspiration by 4% against the background of a decrease in evaporation because of lesser soil moisture content and an increase in moisture losses for infiltration because of permafrost degradation. The observed changes in water balance may have unfavorable environmental effects.