Tobacco is a significant economic crop cultivated in various regions of China. Arbuscular mycorrhizal fungi (AMF) can establish a symbiotic relationship with tobacco and regulate its growth. However, the influences of indigenous AMF on the growth and development of tobacco and their symbiotic mechanisms remain unclear. In this study, a pot inoculation experiment was conducted, revealing that six inoculants - Acaulospora bireticulata(Ab), Septoglomus viscosum(Sv), Funneliformis mosseae(Fm), Claroideoglomus etunicatum(Ce), Rhizophagus intraradices(Ri), and the mixed inoculant (H) - all formed stable symbiotic relationships with tobacco. These inoculants were found to enhance the activities of SOD, POD, PPO, and PAL in tobacco leaves, increase chlorophyll content, IAA content, CTK content, soluble sugars, and proline levels while reducing malondialdehyde content. Notably, among these inoculants, Fm exhibited significantly higher mycorrhizal infection density, arbuscular abundance, and soil spore density in the root systems of tobacco plants compared to other treatments. Membership function analysis confirmed that Fm had the most pronounced growth-promoting effect on tobacco. The transcriptome analysis results of different treatments of CK and inoculation with Fm revealed that 3,903 genes were upregulated and 4,196 genes were downregulated in the roots and stems of tobacco. Enrichment analysis indicated that the majority of these genes were annotated in related pathways such as biological processes, molecular functions, and metabolism. Furthermore, differentially expressed genes associated with auxin, cytokinin, antioxidant enzymes, and carotenoids were significantly enriched in their respective pathways, potentially indirectly influencing the regulation of tobacco plant growth. This study provides a theoretical foundation for the development and application of AMF inoculants to enhance tobacco growth.

This research explores the stabilization of clay soil through the application of geopolymer binder derived from silicomanganese slag (SiMnS) and activated by sodium hydroxide (NaOH). This research aims to evaluate the effects of key parameters, including the percentage of slag, the activator-to-stabilizer ratio, and curing conditions (time and temperature), on the mechanical properties of the stabilized soil. Unconfined compressive strength (UCS) tests were conducted to assess improvements in soil strength, while scanning electron microscopy (SEM) was employed to analyze the microstructural changes and stabilization mechanisms. The results demonstrated that clay soil stabilized with SiMnS-based geopolymers exhibited significant strength enhancement. Specifically, the sample stabilized with 20% SiMnS and an activator-to-slag ratio of 1.6, cured at room temperature for 90 days, achieved a UCS of 27.03 kg & frasl;cm2. The uniaxial strength was found to be positively correlated with the SiMnS content, activator ratio, curing time, and temperature. Additionally, the strain at failure remained below 1.5% for all samples, indicating a marked improvement in soil stiffness. SEM analysis revealed that geopolymerization led to the formation of a dense matrix, enhancing soil particle bonding and overall durability. These results emphasize the potential of SiMnS-based geopolymers as a sustainable and effective soil stabilizer for geotechnical applications.

Fragile fruits, which are prone to mechanical damage and microbial infection, necessitate protective materials that possess both cushioning and antimicrobial properties. In this study, we present a novel genipin-crosslinked chitosan/gelatin aerogel (CS/GEL/GNP) synthesized through direct mixing and free-drying techniques. The mechanical properties and cushioning capacities of the CS/GEL/GNP aerogel were thoroughly characterized, alongside an evaluation of its antimicrobial efficacy. The composite aerogel demonstrated remarkable compressibility and shape recovery characteristics. In a transportation simulation test, the aerogel effectively protected strawberries from mechanical damage. Furthermore, the composite aerogel exhibited enhanced antimicrobial activities against Escherichia coli, Staphylococcus aureus and Botrytis cinerea in vitro. The quality of strawberries was successfully maintained at ambient temperature when packaged with the CS/GEL/GNP. Notably, the aerogel could be completely degraded in the soil within 21 days and is nontoxic to cells. Consequently, the dual-functional CS/GEL/GNP aerogel presents a promising option for packaging materials aimed at protecting delicate fruits.

The foundation soil below the structure usually bears the combined action of initial static and cyclic shear loading. This experimental investigation focused on the cyclic properties of saturated soft clay in the initial static shear stress state. A range of constant volume cyclic simple shear tests were performed on Shanghai soft clay at different initial static shear stress ratios (SSR) and cyclic shear stress ratios (CSR). The cyclic behavior of soft clay with SSR was compared with that without SSR. An empirical model for predicting cyclic strength of soft clay under various SSR and CSR combinations was proposed and validated. Research results indicated that an increase of shear loading level, including SSR and CSR, results in a larger magnitude of shear strain. The response of pore water pressure is simultaneously dominated by the amplitude and the duration of shear loading. The maximum pore water pressure induced by smaller loading over a long duration may be greater than that under larger loading over a short duration. The initial static shear stress does not necessarily have a negative impact on cyclic strength. At least, compared to cases without SSR, the low-level SSR can improve the deformation resistance of soft clay under the cyclic loading. For the higher SSR level, the cyclic strength decreases with the increase of SSR.

Expansive soil, characterized by significant swelling-shrinkage behavior, is prone to cracking under wet-dry cycles, severely compromising engineering stability. This study combines experimental and molecular dynamics (MD) simulation approaches to systematically investigate the improvement effects and micromechanisms of polyvinyl alcohol (PVA) on expansive soil. First, direct shear tests were conducted to analyze the effects of PVA content (0 %-4 %) and moisture content (30 %-50 %) on the shear strength, cohesive force, and internal friction angle of modified soil. Results show that PVA significantly enhances soil cohesive force, with optimal improvement achieved at 3 % PVA content. Second, wet-dry cycle experiments revealed that PVA effectively suppresses crack propagation by improving tensile strength and water retention. Finally, molecular dynamics simulations uncovered the distribution of PVA between montmorillonite (MMT) layers and its influence on interfacial friction behavior. The simulations demonstrated that PVA forms hydrogen bonding networks, enhancing interlayer interactions and frictional resistance. The improved mechanical performance of PVAmodified soil is attributed to both nanoscale bonding effects and macroscale structural reinforcement. This study provides theoretical insights and technical support for expansive soil stabilization.

Shallow cut-and-cover underground structures, such as subway stations, are traditionally designed as rigid boxes (moment-resisting connections between the main structural members), seeking internal hyperstaticity and high lateral (transverse) stiffness to achieve important seismic capacity. However, since seismic ground motions impose racking drifts, this proved rather prejudicial, with great structural damage and little resilience. Therefore, two previous papers proposed an opposite strategy seeking low lateral (transverse) stiffness by connecting the structural elements flexibly (hinging and sliding). Under severe seismic inputs, these structures would accommodate racking without significant damage; this behaviour is highly resilient. The seismic resilience of this solution was numerically demonstrated in the well-known Daikai station (Kobe, Japan) and a station located in Chengdu (China). This paper is a continuation of these studies; it aims to extend, deepen, and ground this conclusion by performing a numerical parametric study on these two stations in a wide and representative set of situations characterised by the soil type, overburden depth, engineering bedrock position, and high- and lowlateral-stiffness of the stations. The performance indices are the racking displacement and the structural damage (quantified through concrete damage variables). The findings of this study validate the previous remarks and provide new insights.

Moderate nitrogen addition can enhance plant growth performance under salt stress. However, the regulatory effects of nitrogen addition on the growth of the leguminous halophyte medicinal plant, Sophora alopecuroides, under salt stress remain unclear. In this study, a two-factor pot experiment with different NaCl levels (1 g/kg, 2 g/kg, 4 g/kg) and NH4NO3 levels (0 mg/kg, 32 mg/kg, 64 mg/kg, 128 mg/kg) was set up to systematically study the response of S. alopecuroides plant phenotype, nodulation and nitrogen fixation characteristics, nitrogen (N), phosphorus (P), potassium (K) nutrient absorption and utilization efficiency, plant biomass and nutrient accumulation to nitrogen addition under salt stress. The results demonstrated that under mild (1 g/kg NaCl) and moderate (2 g/kg NaCl) salt stress, S. alopecuroides exhibited a relatively low nitrogen demand. Specifically, low (32 mg/kg N) and medium (64 mg/kg N) nitrogen levels significantly enhanced nodule nitrogenase activity and nitrogen fixation capacity. Furthermore, the uptake of essential nutrients, including N, P, and K, in the aboveground biomass was markedly increased, which in turn promoted the accumulation of major nutrients such as crude protein, crude fat, and alkaloids, as well as overall biomass production. However, under severe (4 g/kg NaCl) salt stress, S. alopecuroides exhibited a preference for low nitrogen levels (32 mg/kg N). Under S3 conditions, excessive nitrogen application (e.g., 64 mg/kg and 128 mg/kg N) exacerbated the damage caused by salt stress, leading to significant inhibition of nitrogen fixation and nutrient uptake. Consequently, this resulted in a substantial reduction in biomass. This study provides a theoretical basis for nitrogen nutrition management of S. alopecuroides under salt stress conditions and offers valuable insights for optimizing fertilization and nutrient management strategies in saline-alkali agricultural production.

Nanoplastics (NPs) and zinc (Zn), both widespread in soil environments, present considerable risks to soil biota. While NPs persist environmentally and act as vectors for heavy metals like Zn, their combined toxicity, especially in soil invertebrates, remains poorly understood. This study evaluates the individual and combined effects of Zn and NPs on earthworm coelomocytes and explores their interactions with Cu/Zn-superoxide dismutase (SOD), an antioxidant enzyme. Molecular docking revealed that NPs bind near the active site of SOD through pi-cation interactions with lysine residues, further stabilized by neighboring hydrophobic amino acids. Viability assays indicated that NPs alone (20 mg/L) had negligible impact (94.54 %, p > 0.05), Zn alone (300 mg/L) reduced viability to 80.02 %, while co-exposure reduced it further to 73.16 %. Elevated levels of reactive oxygen species (ROS) and malondialdehyde (MDA) levels were elevated to 186 % and 173 % under co-exposure, alongside greater antioxidant enzyme disruption, point to synergistic toxicity. Dynamic light scattering and zeta potential (From -13 to -7 mV) analyses revealed larger particle sizes in the combined system, indicative of enhanced protein interactions. Conformational changes in SOD, such as alpha-helix loss and altered fluorescence, further support structural disruption. These findings demonstrate that co-exposure to NPs and Zn intensifies cellular and protein-level toxicity via integrated physical and biochemical mechanisms, providing critical insight into the ecological risks posed by such co-contaminants in soil environments.

This paper deals with the contribution of the soil-structure interaction (SSI) effects to the seismic analysis of cultural heritage buildings. This issue is addressed by considering, as a case study, the Mosque-Cathedral of Cordoba (Spain). This study is focussed on the Abd al-Rahman I sector, which is the most ancient part, that dates from the 8th century. The building is a UNESCO World Heritage Site and it is located in a moderate seismic hazard zone. It is built on soft alluvial strata, which amplifies the SSI. Since invasive tests are not allowed in heritage buildings, in this work a non-destructive test campaign has been performed for the characterisation of the structure and the soil. Ambient vibration tests have been used to calibrate a refined 3D macro-mechanical-based finite element model. The soil parameters have been obtained through an in situ geotechnical campaign, that has included geophysical tests. The SSI has been accounted for by following the direct method. Nonlinear static and dynamic time-history analyses have been carried out to assess the seismic behaviour. The results showed that the performance of the building, if the SSI is accounted for, is reduced by up to 20 % and 13 % in the direction of the arcades and in the perpendicular direction, respectively. Also, if the SSI is taken into account, the damage increased. This study showed that considering the SSI is important to properly assess the seismic behaviour of masonry buildings on soft strata. Finally, it should be highlighted that special attention should be paid to the SSI, which is normally omitted in this type of studies, to obtain a reliable dynamic identification of the built heritage.

Seismic risk assessment of code-noncompliant reinforced concrete (RC) frames faces significant challenges due to structural heterogeneity and the complex interplay of site-specific hazard conditions. This study aims to introduce a novel framework that integrates three key concepts specifically targeting these challenges. Central to the methodology are fragility fuses, which employ a triplet of curves-lower bound, median, and upper bound-to rigorously quantify within-class variability in seismic performance, offering a more nuanced representation of code-noncompliant building behavior compared to conventional single-curve approaches. Complementing this, spectrum-consistent transformations dynamically adjust fragility curves to account for regional spectral shapes and soil categories, ensuring site-specific accuracy by reconciling hazard intensity with local geotechnical conditions. Further enhancing precision, the framework adopts a nonlinear hazard model that captures the curvature of hazard curves in log-log space, overcoming the oversimplifications of linear approximations and significantly improving risk estimates for rare, high-intensity events. Applied to four RC frame typologies (2-5 stories) with diverse geometries and material properties, the framework demonstrates a 15-40 % reduction in risk estimation errors through nonlinear hazard modeling, while spectrum-consistent adjustments show up to 30 % variability in exceedance probabilities across soil classes. Fragility fuses further highlight the impact of structural heterogeneity, with older, non-ductile frames exhibiting 25 % wider confidence intervals in performance. Finally, risk maps are presented for the four frame typologies, making use of non-linear hazard curves and spectrumconsistent fragility fuses accounting for both local effects and within-typology variability.