Forest soil is crucial in climate change mitigation, food security, and biogeochemical nutrient cycling. Mixed Sal forests enhance soil organic matter, improve nutrient availability, and regulate pH dynamics. However, anthropogenic disturbances, including deforestation and land-use changes, significantly alter forest cover, leading to shifts in soil physicochemical and microbial properties. These impacts necessitate rigorous monitoring and comprehensive assessment. Therefore, we investigated the effects of contrasting conditions- closed (no human activities) and open (human interferences) mixed Sal Forest on the vertical and seasonal dynamics of microbial biomass carbon (SMBC). Results revealed that the closed mixed Sal Forest had significantly higher SMBC than the open mixed Sal Forest across the soil profile (D1-D5) with a strong seasonal effect. Closed mixed Sal Forest had 60% higher SMBC in D1 than open mixed Sal Forest while it reduced with depth and 17.1 to 56.7% higher SMBC in the subsurface to bottom-most soil profile (D2-D5). Moreover, SMBC was higher in the monsoon period in both forests. The SMBC reduced by 24.2 to 45.1% in the post-monsoon period while reduction was more intense in the pre-monsoon period (48.1 to 68.2%) compared to the monsoon period under closed mixed Sal Forest. Similarly, the decline was more intense in the open mixed Sal Forest, where SMBC declined 12.1 to 54% in the post-monsoon period and 56.1 to 76.2% in the pre-monsoon period compared to the monsoon period. The study indicates that human interference in mixed Sal forests leads to loss of forest cover, negatively affecting microbiological properties and reducing soil fertility, which weakens the forest's resilience to climate change. Additionally, SMBC exhibits seasonal variations, reflecting responses to environmental conditions. These results underline the need to reduce human disturbances and enhance forest conservation strategies to ensure soil sustainability and ecosystem stability.

The frequency of forest fires has increased dramatically due to climate change. The occurrence of forest fires affects the carbon and nitrogen cycles and react to climate change to form a positive feedback mechanism. These effects further impact the distribution of microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) and the soil microbial community structure. In addition, permafrost degradation can significantly affect the microorganisms in the soil. Based on these findings, this review examines the effects of fire intensity and post-fire recovery time on permafrost, the soil microbial community, MBC, MBN, and their interrelationships. This review demonstrated that (1) fires alter the condition of surface vegetation, reduce the organic layer thickness, redistribute snow, accelerate permafrost degradation, and even lead to permanent changes, where the restoration of the pre-fire state would require several decades or even centuries; (2) soil microbial community structure, soil MBC, and MBN negatively correlate with fire intensity, and the effects become more pronounced with increasing fire intensity; and (3) the structural diversity and stability of the soil microbial community were improved with time, and the amount of MBC and MBN increases as the years after a fire go by; it would still take more than ten years to recover to the pre-fire level. However, the relationship between permafrost degradation and soil microbes after forest fires is still unclear due to a lack of quantitative research on the mechanisms underlying the changes in soil microorganisms resulting from fire-induced permafrost degradation. Therefore, expanding quantitative studies and analyses of the mechanisms of interactions between forest fires, permafrost, and soil microorganisms can provide a scientific basis for understanding ecosystem carbon pools and dual-carbon targets in Arctic-boreal permafrost regions.

Cinnamaldehyde is a natural compound extracted from cinnamon bark essential oil, acclaimed for its versatile properties in both pharmaceutical and agricultural fields, including antimicrobial, antioxidant, and anticancer activities. Although potential of cinnamaldehyde against plant pathogenic bacteria like Agrobacterium tumefaciens and Pseudomonas syringae pv. actinidiae causative agents of crown gall and bacterial canker diseases, respectively has been documented, indepth studies into cinnamaldehyde's broader influence on plant pathogenic bacteria are relatively unexplored. Particularly, Pectobacterium spp., gram -negative soil -borne pathogens, notoriously cause soft rot damage across a spectrum of plant families, emphasizing the urgency for effective treatments. Our investigation established that the Minimum Inhibitory Concentrations (MICs) of cinnamaldehyde against strains P. odoriferum JK2, P. carotovorum BP201601, and P. versatile MYP201603 were 250 pg/ml, 125 pg/ml, and 125 pg/ml, respectively. Concurrently, their Minimum Bactericidal Concentrations (MBCs) were found to be 500 pg/ml, 250 pg/ml, and 500 pg/ml, respectively. Using RNA -sequencing analysis, we identified 1,907 differentially expressed genes in P. carotovorum BP201601 treated with 500 pg/ml cinnamaldehyde. Notably, our results indicate that cinnamaldehyde upregulated nitrate reductase pathways while downregulating the citrate cycle, suggesting a potential disruption in the aerobic respiration system of P. carotovorum during cinnamaldehyde exposure. This study serves as a pioneering exploration of the transcriptional response of P. carotovorum to cinnamaldehyde, providing insights into the bactericidal mechanisms employed by cinnamaldehyde against this bacterium.