Drought stress significantly inhibits the growth of Astragalus mongholicus, leading to reduced biomass, decreased photosynthetic efficiency, and exacerbated oxidative damage. In our study, the accumulation of saponins and flavonoids in Astragalus roots markedly increased under moderate drought stress. These secondary metabolites further reshaped the rhizosphere microbial community structure, significantly increasing its diversity and interaction network complexity. Notably, drought stress enriched beneficial bacterial genera such as Rhizobium and Pseudomonas in the rhizosphere soil. Combined with the isolation of culturable microorganisms and the cooccurrence network of the rhizosphere bacterial community, we constructed a 13-strain synthetic community (SynCom) and simplified it to 7 strains. Compared with the noninoculated control, under moderate drought stress, inoculation with the simplified SynCom significantly increased plant growth, increasing the aboveground fresh weight by 50.10 %, dry weight by 55.29 %, and underground fresh weight by 76.40 %. Similarly, plants treated with the synthetic community presented significant increases in aboveground fresh weight and dry weight compared with those of the noninoculated control, with increases of 46.98 % and 61.54 %, respectively. Moreover, inoculation with the simplified community significantly reduced the content of malondialdehyde (MDA) and improved the catalase (CAT) and peroxidase (POD) activities and leaf photosynthetic parameters (Fv/ Fm and Y(II)) of Astragalus. Our findings provide new insight into improving the yield and quality of Astragalus and highlight the potential of synthetic rhizosphere microbial communities for assisting plants in coping with abiotic stress.

Salt-affected soils severely decrease agricultural productivity by reducing the uptake of water and nutrients by plants, toxic ions accumulation and soil structure degradation. The sustainable synthesis of hybrid nanospheres through green approaches has emerged as an effective strategy to enhance crop productivity and improve tolerance to abiotic stress. However, the defensive functions and fundamental mechanisms of green synthesized calcium-doped carbon nano-spheres in protecting maize against salt stress remain elusive. Thus, calcium-doped carbon nanospheres were innovatively synthesized by doping calcium oxide nanoparticles (CaO NPs) with lignin nanoparticles (LNPs) which were further analyzed using Fourier Transform Infrared Spectroscopy (FT-IR), Energy Dispersive X-Ray Spectroscopy (EDX), Field Emission Scanning Electron Microscopy (FE-SEM) and Transmission Electron Microscopy (TEM). These analyses validated the successful doping of Ca@CNs, elucidating the purity and morphology of the hybrid nanospheres. More importantly, the effect of Ca@CNs on maize plants under NaCl stress, unreported so far, was examined. Results of the current study showed that treating salt-stressed plants with Ca@CNs significantly improved maize growth and biomass accumulation by enhanced absorption of minerals and improved photosynthetic efficiency. Furthermore, Ca@CNs application has also reduced NaCl-induced oxidative damage by enhancing antioxidant defense mechanisms and maintaining cellular integrity, resulting in improved resistance to salt stress. Moreover, Ca@CNs substantially up-regulated the expression of salt-tolerant genes ZmNHX3, CBL, ZmHKT1, and MAPK1, as well as genes involved in lignin biosynthesis such as 4CL2, PAL1, CCR, and COMT, in both shoot and root tissues. Conversely, the expression levels of genes Zm00001d003114, Zm0001d026638, Zm00001d028582 and Zm00001d051069 associated with Ca2 +-responsive SOS3 pathway were all down-regulated under NaCl treatment, while up-regulated in the presence of Ca@CNs along with NaCl. The observed changes in transcript levels of these genes highlight the potential of Ca@CNs in alleviating NaCl toxicity. These results demonstrated that the green synthetic Ca@CNs can significantly alleviate salt stress and promote plant growth in saline environments, which will provide a new strategy for the utilization of nanoparticles in agriculture to maintain sustainable agriculture and improve crop yield.

Reactive magnesium oxide (MgO) and ground granulated blast furnace slag (GGBS) are cementitious materials introduced into sludge solidification, which not only reutilizes solid waste but also reduces cement consumption. Through the carbonation of reactive MgO and GGBS, the strength of the solidified sludge is further improved and CO2 is stably sequestrated in carbonate minerals. This paper investigates the strength and microstructural development and CO2 uptake of solidified sludge with varying water content, binder content, and ratio of MgO to GGBS. According to unconfined compressive strength (UCS) tests, when the binder content is 20% and the ratio of reactive MgO to GGBS is 2 & ratio;8, the strength of carbonated samples increases the most, which is six times that of the sample without reactive MgO. With binder content, the CO2 uptake of sample increases up to 2.1 g. Scanning electron microscope (SEM), X-ray diffractometer (XRD), and thermogravimetry-differential thermogravimetry analysis (TG-DTG) tests were conducted to systematically elucidate the micromechanism of carbonation of sludge solidified by reactive MgO and GGBS. Various carbonation and hydration products enhance the soil strength through filling pores and integrating fine particles into bulk aggregates. As the ratio of reactive MgO to GGBS increases, dypingite and hydromagnesite were converted into nesquehonite with better morphological integrity, and thus strengthens the soil skeleton. Diverse calcium carbonate polymorphs from carbonated GGBS also promote sludge strength growth and CO2 sequestration. Test results indicate that the addition of reactive MgO further improves the hydration and carbonation properties of GGBS, so the CO2 uptake grows with the ratio of reactive MgO to GGBS. The synergistic effect of reactive MgO and GGBS increases the carbonation performance of the mixed binder, so likewise the compressive strength.

Sodium hydroxide (NaOH)-sodium silicate-GGBS (ground granulated blast furnace slag) effectively stabilises sulfate-bearing soils by controlling swelling and enhancing strength. However, its dynamic behaviour under cyclic loading remains poorly understood. This study employed GGBS activated by sodium silicate and sodium hydroxide to stabilise sulfate-bearing soils. The dynamic mechanical properties, mineralogy, and microstructure were investigated. The results showed that the permanent strain (epsilon(p)) of sodium hydroxide-sodium silicate-GGBS-stabilised soil, with a ratio of sodium silicate to GGBS ranging from 1:9 to 3:7 after soaking (0.74%-1.3%), was lower than that of soil stabilised with cement after soaking (2.06%). The resilient modulus (E-d) and energy dissipation (W) of sodium hydroxide-sodium silicate-GGBS-stabilised soil did not change as the ratio of sodium silicate to GGBS increased. Compared to cement (E-d = 2.58 MPa, W = 19.96 kJ/m(3)), sulfate-bearing soil stabilised with sodium hydroxide-sodium silicate-GGBS exhibited better E-d (4.84 MPa) and lower W (15.93 kJ/m(3)) at a ratio of sodium silicate to GGBS of 2:8. Ettringite was absent in sodium hydroxide-sodium silicate-GGBS-stabilised soils but dominated pore spaces in cement-stabilised soil after soaking. Microscopic defects caused by soil swelling were observed through microscopic analysis, which had a significant negative impact on the dynamic mechanical properties of sulfate-bearing soils. This affected the application of sulfate-bearing soil in geotechnical engineering.

Phytoremediation is a promising approach grounded in green and sustainable development principles for decontaminating water and soil. Among the studied duckweed species (Spirodela polyrhiza, Wolffia arrhizal, and Lemna minor), S. polyrhiza exhibited the highest zinc removal efficiency of 88.50% by day 7, followed by L. minor and W. arrhiza with removal efficiency of 78.69 and 38.59%, respectively. This study investigated the effects of environmental factors, including initial zinc ion concentration (50, 100, 150, 200, and 250 mg/L), solution pH (pH 5, 6, 7, and 8), and macrophytes mass (5, 10, 15, 20, and 25 g) on the phytoremediation of the zinc ion from synthetic wastewater by S. polyrhiza. The process effectively treated 500 mL of synthetic wastewater containing 100 ppm zinc ion and the process could be enhanced to achieve the removal efficiency of 90% by adjusting the solution pH to slightly acidic (pH 5) and increasing the mass of duckweed to its saturation point (20 g). Excessive zinc intake by duckweed led to chlorophyll reduction, negatively impacting the duckweed growth rate. Scanning electron microscopy (SEM) analysis revealed that the duckweed fronds' surface became uneven after the treatment, with the irregular small particles attached due to cellular damage. The energy dispersive X-ray (EDX) analysis confirmed the successful uptake and accumulation of zinc in the duckweed cells from the synthetic wastewater. In conclusion, duckweed-based phytoremediation demonstrates significant potential for removing zinc ion from wastewater, at low and moderate concentrations.

Lateritic clay is widely distributed in southern China, and its strength is greatly affected by water content. The elevated moisture content in lateritic clay during monsoon periods frequently results in insufficient shear strength for standard engineering applications. Large quantities of solid waste, including steel slag, fly ash, and granulated blast furnace slag, are produced as industrial by-products. This paper is based on the backfilling resource utilization of steel slag, fly ash, and ground-granulated blast-furnace slag as lateritic clay improvement admixtures, along with the stress-strain behavior, strength characteristics, and microstructure of steel-slag-modified lateritic clay, fly-ash-modified lateritic clay, and ground-granulated blast-furnace slag-modified lateritic clay, by combining uniaxial compression tests, straight shear tests, and scanning electron microscopy observation. The experimental results were analyzed to determine the appropriate dosages of three kinds of solid waste and their mechanisms in lateritic clay modification. The results indicate that the unconfined compressive strength of SS-modified lateritic clay exhibited an increase with an increase in SS dosage in the range of 1-7%, the unconfined compressive strength of FA-modified lateritic clay showed an increase with an increase in FA dosage in the range of 1-5%, and the unconfined compressive strength of GGBFS-modified lateritic clay increased with an increase in the use of GGBFS in the range of 1-5%. Under the condition of a 7-day curing age, the unconfined compressive strength of lateritic clay modified with 7% SS increased by approximately 397%, while that modified with 5% FA and 5% GGBFS exhibited increases of about 187% and 185%, respectively. The stress-strain relationship of fly-ash and blast-furnace slag-modified lateritic clays showed elastic-plastic deformation. But the stress-strain behavior of steel-slag-modified lateritic clay at a steel slag dose greater than 5% and a maintenance age greater than 7 days showed elastic deformation. Analyzing the SEM images shows that the more hydration products are generated, the relatively higher the unconfined compressive strength of modified lateritic clay is, and the form of deformation of modified lateritic clay is closer to elastic deformation. Through comparative analysis of modified lateritic clay samples, this study elucidates the property-altering mechanisms of waste powder additives, guiding their engineering utilization.

This research examines the influence of blast furnace slag (BFS) on the physico-mechanical properties of compressed earth blocks (CEBs) stabilised with cement and/or lime. A three-factor mixture design is employed to assess the effects of BFS, cement and lime on key properties such as dry density, water content and compressive strength at 28 and 90 days. The study maintains a constant dune sand proportion with soil substitutions up to 20% (420 grams), while the BFS, lime and cement proportions vary with soil substitutions up to 15% (315 g). The findings indicate that mixtures with over 7.5% cement and equal proportions of lime and BFS, as well as a ternary mixture of 10% cement, 2.5% lime and 2.5% BFS, deliver superior strength. Notably, the optimal compressive strength with a high desirability score of 0.93 is achieved using around 14% cement and 1% lime. Proctor curve analysis shows that BFS-cement-lime substitution reduces water content and increases dry density. Statistical analysis confirms the model's robustness in predicting compressive strength, supported by high F-values and low probabilities, and highlights its effectiveness in guiding design decisions. Additionally, the study's evaluation of rupture types offers further insights into material strength and validates adherence to testing standards.

Foamed lightweight soil is widely used in subgrade engineering as a lightweight, high fluidity material. However, due to the use of cement as the main raw material, its cost is relatively high. Therefore, the preparation of foamed lightweight soil by mixing muck excavated at the project site with iron ore tailings (IOT) is not only helpful to reduce costs, but also can promote the efficient and comprehensive utilization of inactive solid waste. In this paper, the fluidity, wet density, compressive strength and specific strength of muck-IOT foamed lightweight soil with different content were tested, and the optimal mixing ratio was selected according to the engineering specifications. Then, through uniaxial and triaxial compression tests, the strength and deformation characteristics of muck-IOT foamed lightweight soil under different dosage, wet density and confining pressure conditions were studied. Finally, the influence mechanism of muck and IOT on the strength and structure of foamed lightweight soil was revealed through Scanning Electron Microscope (SEM) analysis. The research results show that the wet density of foamed lightweight soil prepared by the optimal mixing amount (20% muck and 10% IOT) is 894 kg/m3, and the uniaxial compressive strength is 4.6 MPa. While meeting the requirements of fluidity, the mixing amount of solid waste is higher, with the specific strength increased by 28.12%. In the triaxial compression test, for every 100 kg/m3 increase in wet density, the peak strength and residual strength increase by 1.30 MPa and 1.00 MPa, respectively; For every 200 kPa increase in confining pressure, the peak strength and residual strength increase by 0.27 MPa and 0.32 MPa, respectively. In addition, the shear strength levels of muck-IOT foamed lightweight soil under different normal stress conditions under different wet densities were determined by establishing the linear equations of c and phi related to the wet density. From the microstructure, it can be seen that the pores in the muck-IOT foamed lightweight soil are evenly distributed, resulting in a denser structure and reduced stress concentration, which significantly enhances the material's compressive strength.

Soybean, a globally significant and versatile crop, serves as a vital source of both oil and protein. However, environmental factors such as soil salinization pose substantial challenges to its cultivation, adversely affecting both yield and quality. Enhancing the salt tolerance of soybeans can mitigate yield losses and promote the development of the soybean industry. Members of the plant-specific transcription factor family NAC play crucial roles in plant adaptation to abiotic stress conditions. In this study, we screened the soybean GmNAC family genes potentially involved in the salt stress response and identified 18 GmNAC genes that may function during the early stages of salt stress. Among these, the GmNAC035 gene exhibited a rapid increase in expression within one hour of salt treatment, with its expression being induced by abscisic acid (ABA) and methyl jasmonate (MeJA), suggesting its significant role in the soybean salt stress response. We further elucidated the role of GmNAC035 in soybean salt tolerance. GmNAC035, a nuclear-localized transcriptional activator, enhances salt tolerance when overexpressed in Arabidopsis, reducing oxidative damage and boosting the expression of stress-responsive genes. It achieves this by regulating key stress response pathways, including the SOS pathway, calcium signaling, and ABA signaling. These findings highlight the potential of GmNAC035 as a genetic engineering target to improve crop salt tolerance.

The extensive use of petroleum-based plastics has resulted in critical energy and environmental challenges, driving the pursuit of sustainable and biodegradable bioplastics as ideal alternatives. However, the development of functional bioplastics with superior mechanical strength, water stability, and thermal stability remains a formidable challenge. Herein, inspired by the nacre, a cellulose-based bioplastic was designed with a unique layered architecture and enhanced interfacial interactions,achieved through the self-assembly of carboxymethyl cellulose (CMC) and nano-montmorillonite, while simultaneously forming a chemically and physically double-crosslinked network under the action of TiO2 nanoparticles and citric acid. The resulting bioplastic demonstrated excellent mechanical performance, with the tensile strength reaching 106.83 MPa, representing a 220.09 % improvement over pure CMC-based bioplastic and surpassing the tensile strength of other CMC-based films. Alongside mechanical prowess, it exhibited exceptional water resistance (water absorption reduced to 42.88 %), thermal stability and UV shielding. Furthermore, it was biodegradable and environmentally benign, capable of achieving complete degradation in the soil within three months. This biomimetic strategy provided a novel approach for developing competitive cellulose-based bioplastics, offering a promising alternative to petroleum-derived plastics for everyday applications.