To investigate the coupled time effects of root reinforcement and wet-dry deterioration in herbaceous plant-loess composites, as well as their microscopic mechanisms, this study focused on alfalfa root-loess composites at different growth stages cultivated under controlled conditions. The research included measuring root morphological parameters, conducting wet-dry cycling tests, and performing triaxial compression tests and microscopic analyses (CT scanning and nuclear magnetic resonance) on both bare loess and root-loess composites under various wet-dry cycling conditions. By obtaining shear strength parameters and microstructural indices, the study analyzed the temporal evolution of the shear strength and microstructural characteristics of root-loess composites under wet-dry cycling. The findings indicated that the alfalfa root-loess composite effective cohesion was significantly higher than that of the plain soil in the same growth stage. The alfalfa root-loess composite effective cohesion increased during the growth stage in the same dry-wet cycles. The alfalfa root-loess composite effective cohesion in the same growth stage was negatively correlated with the number of dry-wet cycles. The fatigue damage of the soil's microstructure (pore coarsening, cement hydrolysis, and crack development) increased continuously with the number of dry-wet cycles. However, due to the difference in mechanical properties between roots and the soil, the root-soil composite prevented the deterioration of the soil matrix strength by the dry-wet cycles. As the herbaceous plants grow, the time effect observed in the shear strength of the root-soil composite under the action of dry-wet cycles is the result of the interaction and dynamic coordination between the soil-stabilizing function of the herbaceous plant roots and the deterioration caused by drywet cycles.

Soil erosion can be effectively controlled through vegetation restoration. Specifically, roots combine with soil to form a root-soil complex, which can effectively enhance soil shear strength and play a crucial role in soil reinforcement. However, the relationship between root mechanical traits and chemical compositions and shear performance and reinforcing capacity of soil is still inadequate. In this study, we determined the root chemical properties, performed root tensile tests and root-soil composite triaxial tests using two plants-one with a fibrous root system (ryegrass, Lolium perenne L.) and the other with a tap root system (alfalfa, Medicago sativa L.)-and calculated the factor of safety (FOS). The results revealed that the relationship between root diameter and tensile strength differed among different root characters. Holocellulose content and cellulose content were the main factors controlling the root tensile strength of ryegrass and alfalfa, respectively. The shear properties of the root-soil complex (cohesion (c) and internal friction angle (phi)) are correlated with soil water content (SWC) and root mass density (RMD). Root traits had a more substantial effect on c than phi, with significant differences in c between ryegrass and alfalfa at 7 % and 11 % SWC. The root-soil complex had an optimum RMD, and the maximum increase rates of c were 80.57 % and 34.4 %, respectively. Along slopes, sliding first occurs at the foot of the slope, thus demanding emphasis on protection and reinforcement. On steep gradients with low SWC, ryegrass strongly contributes to soil reinforcement, whereas alfalfa is more effective on gentle gradients with high SWC. The results provide scientific references for species selection for vegetation restoration in the Loess Plateau and a deeper understanding of the mechanical mechanism of soil reinforcement by roots.

Deep-rooted maize plants utilize water and nutrients more effectively, particularly in compacted soil. However, the mechanisms by which different maize genotypes adjust root angles in response to compaction remain underexplored. We conducted a two-year study (2021-2022) on silty loam soils in the North China Plain. We tested two genotypes of maize [one with naturally deep roots (DR) and another with shallow roots (SR)] in compacted (C) and non-compacted (NC) soil. Soil compaction impeded shoot growth in both genotypes; however, DR exhibited better growth than SR. Under compacted conditions, DR maintained steeper root angles and demonstrated superior mechanical strength with larger root cortex areas (increased by 60 %) and stele (increased by 92 %), as well as higher cellulose concentration (up to 146 %). Notably, PIEZO1 gene expression increased significantly (up to 242 %) in DR under compaction, suggesting its role in root structural enhancement, unlike in SR where it remained unchanged. These findings underscore the importance of genetic, anatomical, and biochemical adaptations in maize roots, facilitating their resilience to soil compaction. Such insights could inform the breeding of maize genotypes that are better adapted to diverse soil conditions, potentially boosting agricultural productivity.

Corn rootworms (CRW) are among the most destructive pests in corn production across the Corn Belt, causing considerable damage through larval feeding on roots. While crop rotation and Bt technologies are widely adopted management strategies, their effectiveness is increasingly compromised by the pest's evolution of resistance and behavioral adaptability. Chemical insecticides applied at planting to target larvae directly serve as an additional tool for corn rootworm control. In this study, we evaluated the performance of various insecticides, applied in-furrow, for managing corn rootworms by assessing Node Injury Scale (NIS), lodging rates, and grain yields from 2020 to 2024. We found that Mode of Action (MOA) 3A insecticides (sodium channel modulators), such as Force Evo (tefluthrin) and Capture LFR (bifenthrin), did not provide substantial efficacy in reducing NIS and lodging rates. In contrast, MOA 1B+3A insecticides (acetylcholinesterase (AChE) inhibitors + sodium channel modulators), such as INDEX (chlorethoxyfos + bifenthrin) and AZTEC HC (tebupirimphos + cyfluthrin), significantly reduced CRW larval damage, particularly under high pest pressure in 2020, 2021 and 2023. Differences in insecticide concentrations did not significantly impact larval control efficacy. Additionally, seasonal rainfall during larval hatching and variation in cumulative corn growing degree days (GDD) strongly influenced the root injury and lodging outcomes. Lower GDD likely limits root regeneration, increasing lodging risk under CRW pressure. These findings demonstrate the values of in-furrow insecticides in managing corn rootworms, particularly under high pest pressure and provide valuable insights for developing integrated pest management strategies to sustain effective CRW larval control and improve crop productivity.

Debris flows are a type of natural disaster induced by vegetation-water-soil coupling under external dynamic conditions. Research on the mechanism by which underground plant roots affect the initiation of gulley debris flows is currently limited. To explore this mechanism, we designed 14 groups of controlled field-based simulation experiments. Through monitoring, analysis, calculation, and simulation of the changes in physical parameters, such as volumetric water content, pore-water pressure, and matric suction, during the debris flow initiation process, we revealed that underground plant roots change the pore structure of soil masses. This affects the response time of pore-water pressure to volumetric water content, as well as hydrological processes within soil masses before the initiation of gully debris flows. Underground plant roots increase the peak volumetric water content of rock and soil masses, reduce the rates of increase of volumetric water content and pore-water pressure, and increase the dissipation rate of pore-water pressure. Our results clarify the influence of underground roots on the initiation of gulley debris flows, and also provide support for the initiation warning of gully debris flow. When the peak value of stable volumetric water content is taken as the early warning value, the early warning time of soil with underground plant roots is delayed by 534 to 1253 s. When the stable peak value of pore-water pressure is taken as the early warning value, the early warning time of soil with underground plant roots is delayed by 193 to 1082 s. This study provides a basis for disaster prevention and early warning of gully debris flows in GLP, and also provides ideas and theoretical basis under different vegetation-cover conditions area similar to GLP.

The hydraulic effect of plant roots reduces precipitation infiltration and enhances shallow slope stability. However, after root death and decay, soil permeability increases while water-retention capacity decreases. The time-varying mechanisms governing the hydraulic properties of root-soil composites after root decay remain unclear. This study examines the evolution of soil pore structure following root decay. A time-varying soil water retention curve (SWRC) model was developed to characterize changes in water-retention capacity. Additionally, a time-varying saturated infiltration coefficient model and a permeability coefficient prediction model were established to describe variations in hydraulic properties. A one-dimensional soil column infiltration test was conducted on root-soil composites at different stages of root decay to investigate the time-dependent changes in hydraulic properties. The reliability of the proposed models was validated using experimental results. The findings indicate the following: After root death, root biomass, diameter, length, and number decreased with increasing decay time, stabilizing after four months. Root decay led to a reduction in root volume ratio, which altered soil structure and enhanced the permeability of root-soil composites. Longer decay periods increased soil porosity, modifying the soil water characteristic curve and reducing water-retention capacity. Creeping roots decayed more significantly than fibrous roots due to their distinct morphological traits, making changes in hydraulic properties more pronounced in the topsoil. Therefore, plant root decay negatively affects soil hydraulic properties by continuously altering soil pore structure. These findings provide a crucial foundation for understanding the time-dependent mechanisms of hydraulic property variations in root-soil composites during plant root decay.

In the loess tableland, gully slope instability induces severe soil erosion and land degradation, yet the synergistic effects of dominant vegetation under varying restoration modes combined with dynamic rainfall regimes and topographic variations on gully slope stabilization mechanisms remain inadequately quantified. Therefore, the dominant vegetation species under natural (NR) and artificial restoration (AR) was chosen as the object. Through field sampling, root-soil complex mechanical experiments, and numerical simulations, the protection effect of dominant vegetation under different restoration modes combination with rainfall and topographic variations was investigated. The result revealed significant differences in basic soil physical properties, root morphological characteristics, root and root-soil complex mechanical properties among five dominant vegetated plots under the different restoration modes (P < 0.05). The soil properties in the Scop plot under AR were slightly better than those in the other plots. The roots in the Spp plot developed better under NR. The shear strength of Lespedeza bicolor Turcz. was the highest under NR. The tensile strength of Digitaria sanguinalis (L.) Scop. was greatest under AR. The tensile force and tensile strength of single roots exhibited a significant positive linear correlation and a significant negative exponential correlation, with root diameter, respectively (P < 0.01). For the unstable gully slopes (F-s < 1.0), maximum displacement occurred at the slope foot, where tensile shear failure dominated, while the interior experienced compressive yielding. The grey relational analysis identified rainfall intensity as the primary destabilizing factor, followed by dominant vegetation species, slope height, and slope gradient. Notably, when rainfall intensity reaches or exceeds 0.06 m/h, or when slope height exceeds 20 m combined with long-duration rainfall, the regulatory impacts of dominant vegetation under different restoration modes on the gully slope stability are substantially diminished and become negligible. This study provides a theoretical basis for gully slope protection and ecological environmental construction in loess tableland.

Soil microarthropods affect soil ecosystems in a manner that may contribute to balancing the goals of building soil health and controlling weeds in organic agricultural systems. While soil microarthropod feeding behavior can affect plant growth, their impacts on plant communities in agricultural systems are largely unknown. A greenhouse experiment was conducted to investigate the impacts of microarthropods on weed communities. A model weed seed bank was used in each mesocosm, which included yellow foxtail (Setaria pumila (Poir.) Roem&Schult.), giant foxtail (Setaria faberi Herrm.), Powell amaranth (Amaranthus powellii S. Watson), water-hemp (Amaranthus tuberculatus (Moq.) Sauer), common lambsquarters (Chenopodium album L.), and velvetleaf (Abutilon theophrasti Medik.). The study included three treatments: Collembola (Isotomiella minor, Schaffer 1896) abundance (none, low, high), soil microbial community (sterilized/non-sterilized), and fertilizer (presence/ absence of compost). A lab experiment examining individual weed species interactions with I. minor was conducted to elucidate the mechanisms driving the greenhouse experiment findings. Twenty seeds of each weed species were placed on moistened germination paper in containers with varying I. minor abundance levels (none, low, high, very high). Seed germination was recorded after five and seven days. In the greenhouse, the presence of I. minor increased total weed emergence during the first two weeks, but this effect diminished after three weeks. Increasing I. minor abundances generally decreased weed biomass, though this effect was greater in the non-sterilized soil. In the non-sterilized soil, I. minor presence decreased total aboveground weed biomass production by up to 23 %. The Amaranthus species, Powell amaranth and waterhemp, drove this effect with a 55 % and 32 % reduction in biomass, respectively. In tandem, the Amaranthus species had reduced abundances in the presence of I. minor. I. minor increased yellow foxtail germination in the lab, while not affecting the other weed species. This suggests that their effects on the Amaranthus weeds in the greenhouse were likely not caused by direct effects on germination, but instead through nutrient cycling or root herbivory. The proposed mechanism underlying these interactions is that I. minor can initially stimulate germination by feeding on seed coats, but when the seed coats are minimal can damage the seedling. Our findings indicate I. minor could impact weed growth in a manner that affects management decisions and outcomes.

Silicon monoxide (SiO) is highly attractive as an anode material for high-energy lithium-ion batteries (LIBs) due to its significantly higher specific capacity. However, its practical application is hindered by substantial volume expansion during cycling, which leads to material pulverization and an unstable solid electrolyte interphase (SEI) layer. Inspired by the natural root fixation in soil, we designed a root-like topological structure binder, cassava starch-citric acid (CS-CA), based on the synergistic action of covalent and hydrogen bonds. The abundant -OH and -COOH groups in CS-CA molecules effectively form hydrogen bonds with the -OH groups on the SiO surface, significantly enhancing the interfacial interaction between CS-CA and SiO. The root-like topological structure of CS-CA with a high tolerance alleviates the mechanical stress generated by the volume changes of SiO. More encouragingly, the hydrogen bond action among CS-CA molecules produces a self-healing effect, which is advantageous for repairing damaged electrodes and preserving their structural integrity. As such, the CS-CA/SiO electrode exhibits exceptional cycling performance (963.1 mA h g-1 after 400 cycles at 2 A g-1 ) and rate capability (558.9 mA h g-1 at 5 A g-1 ). This innovative, topologically interconnected, root-inspired binder will greatly advance the practical application of long-lasting micron-sized SiO anodes. (c) 2025 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights are reserved, including those for text and data mining, AI training, and similar technologies.

In this study, we present an on-chip analytical method using a microfluidic device to characterize the mechanical properties in growing roots. Roots are essential organs for plants and grow under heterogeneous conditions in soil. Especially, the mechanical impedance in soil significantly affects root growth. Understanding the mechanical properties of roots and the physical interactions between roots and soil is important in plant science and agriculture. However, an effective method for directly evaluating the mechanical properties of growing roots has not been established. To overcome this technical issue, we developed a polydimethylsiloxane (PDMS) microfluidic device integrated with a cantilevered sensing pillar for measuring the protrusive force generated by the growing roots. Using the developed device, we analyzed the mechanical properties of the roots in a model plant, Arabidopsis thaliana. The root growth behavior and the mechanical interaction with the sensing pillar were recorded using a time-lapse microscopy system. We successfully quantified the mechanical properties of growing roots including the protrusive force and apparent Young's modulus based on a simple physical model considering the root morphology. (c) 2025 Institute of Electrical Engineers of Japan and Wiley Periodicals LLC.