BackgroundForensic entomotoxicology is a crucial field that studies the effects of drugs and poisons on carrion-feeding insects, particularly in crime investigations. Hydrogen cyanamide, a plant growth regulator, is hazardous and used in agriculture but is limited in some countries due to its high cost and severe toxicity. The terrestrial isopod Porcellio laevis plays a vital role in soil ecosystems and biosystem management. Accordingly, authors aimed to examine the impact of hydrogen cyanamide toxicity on arthropods, specifically Porcellio laevis, Musca domestica (House flies), and Sarcophaga sp. (Flesh flies) visiting decomposing covered/uncovered rat carrions, which could be relevant in forensic investigations. A total of 20 rats were divided into two control (I and II, covered/uncovered) and two treated groups (III and IV, covered/uncovered, euthanized using hydrogen cyanamide). Arthropods were gathered bi-daily during the initial week and then once daily for a duration of 1 month and were assessed for growth rate. Morphological and histological alterations were analyzed using light and electron microscopes.ResultsThe results revealed that hydrogen cyanamide caused a delay in postmortem interval (PMI) by 22-33 h in certain insect species, particularly in uncovered carrion. Severe damage was observed in the carrions of Groups III and IV, specifically Porcellio laevis.ConclusionA scanning electron microscope (SEM) would be beneficial for scrutinizing insects as postmortem toxicological specimens.

Background: Rodents severely damage the ecological environment of grasslands, and rodent mounds of different ages require distinct management strategies. Understanding the age of these mounds aids in formulating targeted restoration measures, which can enhance grassland productivity and biodiversity. Current surveys of rodent mounds rely on ground exposure and mound height to determine their age, which is time-consuming and labor-intensive. Remote sensing methods can quickly and easily identify the distribution of rodent mounds. Existing remote sensing images use ground exposure and mound height for identification but do not distinguish between mounds of different ages, such as one-year-old and two-year-old mounds. According to the existing literature, rodent mounds of different ages exhibit significant differences in vegetation structure, soil background, and plant diversity. Utilizing a combination of vegetation indices and hyperspectral data to determine the age of rodent mounds aims to provide a better method for extracting rodent hazard information. This experiment investigates and analyzes the age, distribution, and vegetation characteristics of rodent mounds, including total coverage, height, biomass, and diversity indices such as Patrick, Shannon-Wiener, and Pielou. Spectral data of rodent mounds of different ages were collected using an Analytical Spectral Devices field spectrometer. Correlation analysis was conducted between vegetation characteristics and spectral vegetation indices to select key indices, including NDVI670, NDVI705, EVI, TCARI, Ant, and SR. Multiple stepwise regression and Random Forest (RF) inversion models were established using vegetation indices, and the most suitable model was selected through comparison. Random Forest modeling was conducted to classify plateau zokor rat mounds of different ages, using both vegetation characteristic indicators and vegetation indices for comparison. The rodent mound classification models established using vegetation characteristic indicators and vegetation indices through Random Forest could distinguish rodent mounds of different ages, with out-of-bag error rates of 36.96% and 21.74%, respectively. The model using vegetation indices performed better. Conclusions: (1) Rodent mounds play a crucial ecological role in alpine meadow ecosystems by enhancing plant diversity, biomass, and the stability and vitality of the ecosystem. (2) The vegetation indices SR and TCARI are the most influential in classifying rodent mounds. (3) Incorporating vegetation indices into Random Forest modeling facilitates a precise and robust remote sensing interpretation of rodent mound ages, which is instrumental for devising targeted restoration strategies.

Fire in the Northern Alps is comparatively rare. Yet, previous human-ignited fire events in subalpine forests up to the treeline have triggered severe fire damage to vegetation and soil. Here, we investigate post-fire vegetation dynamics in the Northern Limestone Alps about 80 years after disturbance. We observed higher species richness in burned compared to unburned vegetation and clearly distinct floristic communities emerging after fire-driven forest removal, with several alpine specialist species uniquely found in the burned subalpine sites. The functional composition of vegetation was also distinct, with higher relative forb cover in burned plots. This difference was likely driven by disturbance-related environmental changes, such as increased light availability, offering safe sites for subalpine and alpine species. Due to a general lack of tree encroachment, we consider this a case of arrested succession after fire. We conclude that the recovery of fire-affected subalpine forests is modulated by complex interactions of climatic and biotic filters producing extreme site conditions, controlling the recolonization of the disturbed areas by forest species while providing safe sites for the establishment of a rich subalpine and alpine low-statured flora. The coupling of disturbance and abiotic filters makes high-elevation treeline ecotones very vulnerable to climate change.

The intensity and frequency of forest fires are increasing in the cultural landscape of central Europe as the climate is becoming warmer and drier. This requires an understanding of natural regeneration processes in forests and the effects of traditional and new approaches to restoring fire-damaged forests; however, it warrants more research in Germany, where large-scale stand-replacing fire is a new phenomenon in recent times. Specifically, early successional plant pioneer communities, such as mosses, influence the regenerating forest system, by providing viable conditions and habitats for subsequent plant species. The present work focuses on the processes that take place in the moss communities undergoing management interventions of a gradient of intervention intensities after fire disturbance. In a plot-based field inventory, we investigated early successional moss communities three years after a forest fire in Brandenburg, Germany. The study area was subjected to various postfire interventions: high intensity site preparation and dense row planting, natural regeneration (no intervention), and a moderate site preparation in combination with low-density group planting approach. Utilizing Bray-Curtis-based nonmetric multidimensional scaling to assess similarities among postfire moss communities, a simplification of moss communities under high-intensity postfire intervention was observed. We found that the diversity and abundance of mosses decreased with the application of high-intensity postfire intervention but increased with the application of moderate postfire interventions. Furthermore, we found a higher share of light-demanding pioneer mosses in areas under high intensity postfire intervention. In areas under moderate or no postfire intervention, more shade-tolerant species were present. We conclude that moderate interventions with low-intensity site preparation and group planting resulted in reduced losses of moss species and coverage in the successional moss community.

Despite the extensive research conducted on plant-soil-water interactions, the understanding of the role of plant water sources in different plant successional stages remains limited. In this study, we employed a combination of water isotopes (delta 2H and delta 18O) and leaf delta 13C to investigate water use patterns and leaf water use efficiency (WUE) during the growing season (May to September 2021) in Hailuogou glacier forefronts in China. Our findings revealed that surface soil water and soil nutrient gradually increased during primary succession. Dominant plant species exhibited a preference for upper soil water uptake during the peak leaf out period (June to August), while they relied more on lower soil water sources during the post-leaf out period (May) or senescence (September to October). Furthermore, plants in late successional stages showed higher rates of water uptake from uppermost soil layers. Notably, there was a significant positive correlation between the percentage of water uptake by plants and available soil water content in middle and late stages. Additionally, our results indicated a gradual decrease in WUE with progression through succession, with shallow soil moisture utilization negatively impacting overall WUE across all succession stages. Path analysis further highlighted that surface soil moisture (0- 20 cm) and middle layer nutrient availability (20- 50 cm) played crucial roles in determining WUE. Overall, this researchemphasizes the critical influence of water source selection on plant succession dynamics while elucidating un- derlying mechanisms linking succession with plant water consumption.

Hurricanes are extreme climatic events frequently affecting tropical regions such as the tropical dry forests (TDFs) in Mexico, where its frequency/intensity is expected to increase toward the year 2100. To answer how resistant is a Mexican tropical dry forest to a high-intensity hurricane, and if its degree of resistance was mediated by its conservation degree, we evaluated the effect of a category 4 hurricane over the tree community, soil nutrients, and soil enzymatic activity in two contrasting TDF ecosystems: Old-Growth Forest (OGF) and Secondary Forest (SF). In general, vegetation richness and diversity showed very high resistance one year after the hurricane, but several structural attributes did not, especially in the OGF where the tree mortality related to vegetation structure and spatial distribution of individuals was higher. Then, in the short term, SF vegetation appeared to be more resistant, whereas the OGF, with more biomass to lose, appeared to be more vulnerable. Conversely, most soil attributes showed low resistance in both stages, but especially in SF which could face more severe nutrient limitations. The response of TDF to high-intensity hurricanes, in terms of above- and belowground processes, was in part dependent on its disturbance level. Moreover, an increase in the intensity/frequency of hurricanes could lead this TDF toward a high nutrient limitation (especially by phosphorus) for the plants and consequently toward a loss of soil functioning, especially in the SF. This eventually could produce a severe degradation in fundamental attributes and functions of the ecosystem.

Steel slag (SS) has many applications, but its immediate reuse is not possible due to its inherent swelling potential and presence of toxic metals. Therefore, it can only be used after the aging process, which can be either natural or artificial. While few large-scale steel plants afford artificial aging, many small-scale ones opt for natural aging through stockpiling of SS. This results in an increase in soil pH to over 12, thus damaging the ecosystem and making it unviable for plant growth. This research focuses on the reclamation of land affected by SS through the formation of a Phyto-barrier using 22 native plant species aided by the application of a 2 % (v/v) solution of the organic amendment. Furthermore, the superior performance of plants belonging to the Fabaceae family was ascertained, while establishing Sesbania grandiflora as an able species for aided-phytoremediation due to its remarkable growth (approximate to 10 ft tall and 33 cm in circumference) during the study period. The CO2 sequestered by the plantation showed that maximum sequestration has been done by Sesbania grandiflora (49.96 kg CO2 / tree/ year), and least by Azadirachta indica (0.35 kg CO2/tree/year). The overall CO2 sequestered by the plantation stood at 3.85 tons/year. A cost-benefit analysis of using aided-phytoremediation indicates an expense of 90 $ per year as the recurring expense, while carbon credits if monetized, would yield 154 $ to 308 $ as returns. The investigations of this study established a new approach to vegetation over SS-affected land, through native species and the application of organic amendment.

Belowground assemblages are closely related to the aboveground vegetation and edaphic properties, which are also driven by dominant plants due to direct and indirect influences. However, the effects of dominant woody plants on the belowground organisms along successional gradients remain poorly understood. Plant and soil samples were collected from an initial herbaceous stage (i.e. alpine meadows) and four stages dominated by woody species, beneath and between patches of the dominant woody plants, to assess the effects of dominant woody plants on the succession of microbial communities along a secondary successional gradient. We quantified herbaceous, edaphic, bacterial, and fungal dissimilarities between stages to explore how dominant woody plants affect bacterial and fungal dissimilarities between stages using structural equation modeling. We found that dominant woody plants generally increase the succession of microbial communities in early stages, but decrease it in late stages. Our results further suggest that the herbaceous dissimilarity between stages plays more important roles than the edaphic one in mediating the effect of dominant woody plants on both bacterial and fungal dissimilarities between stages. Our results provide insight into the relative role of direct and indirect influences on microbial dissimilarity between stages and highlight the importance of dominant woody plants in driving microbial succession. As woody encroachment increases in alpine meadows, the dominant woody plants may have strong consequences on the dynamic of microbial communities, thereby affecting ecosystem functioning.

Exposed surfaces following glacial retreat are ideal field laboratories for studying primary vegetation succession. Many related studies based on ground sampling methods have been performed worldwide in proglacial zones, but studies on species diversity and vegetation succession using aerial photography have been rare. In this study, we investigated soil organic carbon (SOC), total nitrogen (TN), plant species diversity, and fractional vegetation cover (FVC) along a chronosequence within the foreland of Urumqi Glacier No. 1 by combining field sampling and aerial photography. We then analysed soil development and vegetation succession along distance (distance from glacier terminus) and time (terrain age) gradients as well as the relationships between topographic and environmental variables (aspect, slope, SOC, and TN), distance, time, and species distributions. The results indicated that: (1) plant diversity and FVC showed increasing trends with increases in distance and terrain age, whereas soil nutrient content varied nonlinearly; (2) Silene gonosperma, Leontopodium leontopodioides, and Saussurea gnaphalodes were the dominant species in the early, transient, and later succession stages, respectively. Cancrinia chrysocephala occurred in all stages and had a high abundance in the early and later stages; and (3) the relationships of FVC with soil nutrient content were nonlinear. Moreover, distance and site age played important roles in species distribution. These findings confirm that distance and terrain age positively affect vegetation succession. The increase in FVC facilitated the accumulation of soil nutrition, but this trend was affected by the rapid growth of plants. Caryophyllaceae and Asteraceae were the most common plants during the succession stages, and the former tended to colonise in the early succession stage. We conclude that the UAV-based method exhibits a high application potential for assessing vegetation dynamics in glacier forelands, which has a significance for long-term and repeated monitoring on the process of vegetation colonisation and succession in deglariated areas. (C) 2021 Elsevier B.V. All rights reserved.

Climate change is creating widespread ecosystem disturbance across the permafrost zone, including a rapid increase in the extent and severity of tundra wildfire. The expansion of this previously rare disturbance has unknown consequences for lateral nutrient flux from terrestrial to aquatic environments. Lateral loss of nutrients could reduce carbon uptake and slow recovery of already nutrient-limited tundra ecosystems. To investigate the effects of tundra wildfire on lateral nutrient export, we analyzed water chemistry in and around the 10-year-old Anaktuvuk River fire scar in northern Alaska. We collected water samples from 21 burned and 21 unburned watersheds during snowmelt, at peak growing season, and after plant senescence in 2017 and 2018. After a decade of ecosystem recovery, aboveground biomass had recovered in burned watersheds, but overall carbon and nitrogen remained similar to 20% lower, and the active layer remained similar to 10% deeper. Despite lower organic matter stocks, dissolved organic nutrients were substantially elevated in burned watersheds, with higher flow-weighted concentrations of organic carbon (25% higher), organic nitrogen (59% higher), organic phosphorus (65% higher), and organic sulfur (47% higher). Geochemical proxies indicated greater interaction with mineral soils in watersheds with surface subsidence, but optical analysis and isotopes suggested that recent plant growth, not mineral soil, was the main source of organic nutrients in burned watersheds. Burned and unburned watersheds had similar delta N-15-NO3-, indicating that exported nitrogen was of preburn origin (i.e., not recently fixed). Lateral nitrogen flux from burned watersheds was 2- to 10-fold higher than rates of background nitrogen fixation and atmospheric deposition estimated in this area. These findings indicate that wildfire in Arctic tundra can destabilize nitrogen, phosphorus, and sulfur previously stored in permafrost via plant uptake and leaching. This plant-mediated nutrient loss could exacerbate terrestrial nutrient limitation after disturbance or serve as an important nutrient release mechanism during succession.