Uranium/cadmium (U/Cd) pollution poses a significant global environmental challenge, and phytoremediation offers a sustainable solution for heavy metal contamination. However, the mechanisms by which plants survive U/Cd stress remain unclear. Here, we conducted soil culture experiments of moso bamboo seedlings under U/Cd stress (U, Cd and U + Cd) to examine the effects of it on plant growth, mineral metabolism, and rhizosphere micro-environment. Our findings reveal that U/Cd stress inhibits seedling growth, enhances reactive oxygen species damage, and bolsters the antioxidant system. Additionally, Partial Least Squares Path Modeling (PLS-PM) was employed to uncover potential tolerance mechanisms in moso bamboo under U/Cd stress. U/Cd is mainly distributed in the root cell walls and also exists predominantly in the residual state within the roots. Correspondingly, U and Cd significantly disrupt mineral metabolism in plant. Metabolomic analyses indicate that U/ Cd markedly suppress amino acid metabolism pathways, while they stimulate carbon metabolism to mitigate toxicity. Furthermore, U/Cd stress disrupts the rhizosphere microbial community structure, and the competitive interaction of nitrogen functions exists between rhizosphere microorganism and bamboo roots. PLS-PM reveal the U/Cd stress impacts the interaction of the soil-rhizosphere-plant system. Together, these findings offer new insights into the response mechanism of bamboo plants to heavy metal stress, and provide a theoretical foundation for screening heavy metal tolerant plants and managing mining areas.

In this study, we present an on-chip analytical method using a microfluidic device to characterize the mechanical properties in growing roots. Roots are essential organs for plants and grow under heterogeneous conditions in soil. Especially, the mechanical impedance in soil significantly affects root growth. Understanding the mechanical properties of roots and the physical interactions between roots and soil is important in plant science and agriculture. However, an effective method for directly evaluating the mechanical properties of growing roots has not been established. To overcome this technical issue, we developed a polydimethylsiloxane (PDMS) microfluidic device integrated with a cantilevered sensing pillar for measuring the protrusive force generated by the growing roots. Using the developed device, we analyzed the mechanical properties of the roots in a model plant, Arabidopsis thaliana. The root growth behavior and the mechanical interaction with the sensing pillar were recorded using a time-lapse microscopy system. We successfully quantified the mechanical properties of growing roots including the protrusive force and apparent Young's modulus based on a simple physical model considering the root morphology. (c) 2025 Institute of Electrical Engineers of Japan and Wiley Periodicals LLC.

Tree architecture is an important component of forest community dynamics - taller trees with larger crowns often outcompete their neighbors, but they are generally at higher risk of wind-induced damage. Yet, we know little about wind impacts on tree architecture in natural forest settings, especially in complex tropical forests. Here, we use airborne light detection and ranging (LiDAR) and 30 yr of forest inventory data in Puerto Rico to ask whether and how chronic winds alter tree architecture. We randomly sampled 124 canopy individuals of four dominant tree species (n = 22-39). For each individual, we measured slenderness (height/stem diameter) and crown area (m2) and evaluated whether exposure to chronic winds impacted architecture after accounting for topography (curvature, elevation, slope, and soil wetness) and neighborhood variables (crowding and previous hurricane damage). We then estimated the mechanical wind vulnerability of trees. Three of four species grew significantly shorter (2-4 m) and had smaller crown areas in sites exposed to chronic winds. A short-lived pioneer species, by contrast, showed no evidence of wind-induced changes. We found that three species' architectural acclimation to chronic winds resulted in reduced vulnerability. Our findings demonstrate that exposure to chronic, nonstorm winds can lead to architectural changes in tropical trees, reducing height and crown areas. La arquitectura de los & aacute;rboles es un componente importante de la din & aacute;mica de la comunidad forestal: los & aacute;rboles m & aacute;s altos con copas m & aacute;s grandes suelen sobrepasar a sus vecinos, pero por lo general corren m & aacute;s riesgo de sufrir da & ntilde;os inducidos por el viento. Sin embargo, es poco lo que se sabe sobre el impacto del viento en la arquitectura de los & aacute;rboles en entornos forestales naturales, sobre todo en bosques tropicales complejos. En este caso, utilizamos LiDAR y 30 a & ntilde;os de datos de campo en Puerto Rico para preguntarnos si los vientos cr & oacute;nicos alteran la arquitectura de los & aacute;rboles. Se tom & oacute; una muestra aleatoria de 124 individuos del dosel de cuatro especies arb & oacute;reas dominantes (n = 22-39). De cada individuo, medimos la esbeltez (altura/di & aacute;metro) y el & aacute;rea de la copa (m2) y evaluamos si la exposici & oacute;n a vientos cr & oacute;nicos influ & iacute;a en la arquitectura teniendo en cuenta la topograf & iacute;a (curvatura, elevaci & oacute;n, pendiente, humedad del suelo) y las variables del vecindario (aglomeraci & oacute;n y da & ntilde;os previos por huracanes). Luego, estimamos la vulnerabilidad mec & aacute;nica de los & aacute;rboles al viento. En los lugares expuestos a vientos cr & oacute;nicos, tres de las cuatro especies crecieron mucho menos (2-4 m) y tuvieron & aacute;reas de copa m & aacute;s peque & ntilde;as. Cecropia schreberiana, en cambio, no mostr & oacute; indicios de cambios inducidos por el viento. La aclimataci & oacute;n arquitect & oacute;nica de tres especies a los vientos cr & oacute;nicos llevaba a una reducci & oacute;n de la vulnerabilidad. Nuestros hallazgos demuestran que la exposici & oacute;n a vientos cr & oacute;nicos puede provocar cambios arquitect & oacute;nicos en los & aacute;rboles tropicales, reduciendo su altura y la superficie de sus copas.

Zinc (Zn) is a vital micronutrient required for optimal plant growth and soil fertility. Its use in the form of nanoparticles (NPs) has gained significant attention in agricultural applications. Green synthesized Zn-based NPs offer an eco-friendly solution to several conventional problems in agriculture. Several plants, bacteria, fungi and yeast have shown significant potential in fabricating Zn NPs that can provide environmentally friendly solutions in agriculture and the approach is aligned with sustainable agricultural practices, reducing the dependency on harmful agrochemicals. Zn-based NPs act as plant growth promoters, enhance crop yield, promote resilience to abiotic stressors and are efficient crop protection agents. Their role as a smart delivery system, enabling targeted and controlled release of agrochemicals, further signifies their potential use in agriculture. Because agriculture requires repeated applications hence, the toxicological aspects of Zn NPs cannot be ignored. Zn NPs are reported to cause phytotoxicity, including root damage, physiological and biochemical disturbances, and genotoxic effects. Furthermore, exposure to Zn NPs poses risks to soil microbiota, and aquatic and terrestrial organisms potentially impacting the ecosystem. The green synthesis of Zn-based NPs has a promising aspect for advancing sustainable agriculture by reducing agrochemical use and improving crop productivity. Their diverse applications as plant growth promoters, crop protectants and smart delivery systems emphasize their potential. However, the toxicological aspects are essential to ensure the standardization of doses for their safe and effective use. Further research would help address such concerns and help in developing viable and eco-friendly solutions for modern agriculture. (c) 2025 Society of Chemical Industry.

Numerous specimens stored in natural history collections have been involuntarily preserved together with their associated microbiomes. We propose exploiting century-old soils occasionally found on the roots of herbarium plants to assess the diversity of ancient soil microbial communities originally associated with these plants. We extracted total DNA and sequenced libraries produced from rhizospheric soils and roots of four plants preserved in herbaria for more than 120 years in order to characterise the preservation and taxonomic diversity that can be recovered in such contexts. Extracted DNA displayed typical features of ancient DNA, with cytosine deamination at the ends of fragments predominantly shorter than 50 bp. When compared to extant microbiomes, herbarium microbial communities clustered with soil communities and were distinct from communities from other environments. Herbarium communities also displayed biodiversity features and assembly rules typical of soil and plant-associated ones. Soil communities were richer than root-associated ones with which they shared most taxa. Regarding community turnover, we detected collection site, soil versus root and plant species effects. Eukaryotic taxa that displayed a higher abundance in roots were mostly plant pathogens that were not identified among soil-enriched ones. Conservation of these biodiversity features and assembly rules in herbarium-associated microbial communities indicates that herbarium-extracted DNA might reflect the composition of the original plant-associated microbial communities and that preservation in herbaria seemingly did not dramatically alter these characteristics. Using this approach, it should be possible to investigate historical soils and herbarium plant roots to explore the diversity and temporal dynamics of soil microbial communities.

Global warming due to climate change has substantial impact on high-altitude permafrost affected soils. This raises a serious concern that the microbial degradation of sequestered carbon can result in alteration of the biogeochemical cycles. Therefore, the characterization of permafrost affected soil microbiomes, especially of unexplored high-altitude, low oxygen arid region, is important for predicting their response to climate change. This study presents the first report of the bacterial diversity of permafrost-affected soils in the Changthang region of Ladakh. The relationship between soil pH, organic carbon, electrical conductivity, and available micronutrients with the microbial diversity was investigated. Amplicon sequencing of permafrost affected soil samples from Jukti and Tsokar showed that Proteobacteria and Actinobacteria were the dominant phyla in all samples. The genera Brevitalea, Chthoniobacter, Sphingomonas, Hydrogenispora, Clostridium, Gaiella, Gemmatimonas were relatively abundant in the Jukti samples whereas the genera Thiocapsa, Actinotalea, Syntrophotalea, Antracticibcterium, Luteolibacter, Nitrospirillum dominated the Tsokar sample. Correlation analyses highlighted the influence of soil geochemical parameters on the bacterial community structure. PCoA analyses showed that the bacterial beta diversity varied significantly between the sampling locations (PERMANOVA test (F-value: 2.3316; R2 = 0.466, p = 0.001) and similar results were also obtained while comparing genus abundance data using the ANOSIM test (R = 0.345, p = 0.007).

Many studies have investigated the toxic effects of microplastics (MPs) ingested by aquatic animals, but the effects of MPs that adhere to the roots of macrophytes require further exploration. Thus, the present study investigated the dose-dependent toxic effects of adding 10-500 mg/kg of polycaprolactam microplastics (PCM) on allelopathic cyanobacterial inhibition by a wetland macrophyte due to the influence on rhizosphere bacteria in a pot trial. First, comparisons of sterilized and unsterilized Iris pseudacorus rhizosphere soil showed that the unsterilized soil could enhance the root activity and allelopathic inhibition of Microcystis aeruginosa cyanobacteria. Furthermore, adding 50-100 mg/kg PCM to the unsterilized soil significantly altered the abundances of many types of bacteria, and decreased the root activity and bacterial biodiversity in the rhizosphere. Importantly, PCM changed the secondary metabolites profile in the roots, as well as decreasing production of the allelochemical palmitic acid and the allelopathic potential of I. pseudacorus. Moreover, a dominant strain of functional bacterium AAP51 was identified as an allelopathic promoter, isolated, and successfully inoculated into the sterilized soil. The decomposition of PCM produced the toxic monomer caprolactam in the rhizosphere soil at an average rate of 0.067 mg/kgd under treatment with 50 mg/kg PCM. Toxicological testing showed that 5 mg/kg caprolactam inhibited the activities of the dominant bacteria and expression of the allelopathic gene FAD2 to weaken the allelopathic effect of I. pseudacorus. Thus, the findings obtained in this study indicate that PCM inhibited the allelopathic potential of the macrophyte due to the release of toxic caprolactam damaging bacteria in the rhizosphere. Consequently, it is necessary to remove MP pollutants from aquatic ecosystems in order to maintain the strong allelopathic potential of macrophytes and efficiently control cyanobacterial blooms.

Soil salinization has been the major form of soil degradation under the dual influence of climate change and high-intensity human activities, threatening global agricultural sustainability and food security. High salt concentrations induce osmotic imbalance, ion stress, oxidative damage, and other hazards to plants, resulting in retarded growth, reduced biomass, and even total crop failure. Halo-tolerant plant growth promoting rhizobacteria (HT-PGPR), as a widely distributed group of beneficial soil microorganisms, are emerging as a valuable biological tool for mitigating the toxic effects of high salt concentrations and improve plant growth while remediating degraded saline soil. Here, the current status, harm, and treatment measures of global soil salinization are summarized. The mechanism of salt tolerance and growth promotion induced by HT-PGPR are reviewed. We highlight that advances in multiomics technologies are helpful for exploring the genetic and molecular mechanisms of microbiota centered on HT-PGPR to address the issue of plant losses in saline soil. Future research is urgently needed to comprehensively and robustly determine the interaction mechanism between the root microbiome centered on HT-PGPR and salt-stressed plants via advanced means to maximize the efficacy of HT-PGPR as a microbial agent. Halo-tolerant plant growth promoting rhizobacteria (HT-PGPR) are a valuable biological tool for mitigating the toxic effects of high salt concentrations. And the microbiome centered on HT-PGPR is solutions for sustainable agriculture in saline soils.

AimsPlant roots play a crucial role in soil stability and erosion prevention. Most studies currently focus on the macro-biomechanical properties of roots based on apparent diameter or stele size. However, these analyses cannot explain the factors affecting macro-biomechanical properties of roots from an endogenous perspective.MethodsTensile tests, scanning electron micrography (SEM), image-based strain measurement and compositional tests were conducted on roots of typical species (Robinia pseudoacacia, Pinus tabuliformis, Vitex negundo, Syzygium aromaticum) in the Loess Plateau to explore the influence of stele on enhancing and pores on weakening mechanical properties.ResultsRoot breakages in tension can be categorized into simultaneous and successive brittle breakage, with most simultaneous brittle breakages occurring in fine roots and most successive brittle breakages occurring in coarse roots, respectively. The negative regression between tensile strength (Tr) and diameter (Dr) was attributed to the decrease in cellulose content. The positive regression between Tr and stele percentage was attributed to the dominant distribution of cellulose within the stele of root. Pores in plant root could weaken the macro-biomechanical properties, with trees generally having higher porosity than shrubs in this research species. The non-uniformity coefficient (UC) of pores reflected their distribution form. The fine roots, with higher UC, showed more random pore distribution, more scattered macro-biomechanical properties than coarse roots.ConclusionsOur results explained the intrinsic characteristics that influence the macro-biomechanical properties along root diameters. This finding provides valuable insights for understanding the mechanical properties of plant roots and providing soil reinforcement theoretical basis.

A sustainable use of croplands should utilize beneficial services provided by their resident soil microbiome. To identify potentially adverse environmental effects on soil microbiomes in the future, a better understanding of their natural variability is fundamental. Here, we characterized the abundance and diversity of soil microbial communities over 2 years at two-week intervals on three neighboring fields at an operational farm in Northern Germany. Field soils differed in texture (clay, loam) and tillage (soil conservation vs. conventional). PCRamplicon analyses of soil DNA revealed distinct temporal variations of bacteria, archaea, fungi, and protists (Cercozoa and Endomyxa). Annual differences and seasonal effects on all microbial groups were detected. In addition to soil pH, prokaryotic communities varied with total soil C and N, but fungi with temperature and precipitation. The C/N ratio had contrasting effects on prokaryotic phyla and protistan classes, but all fungal phyla responded positively. Irrespective of the sampling date, prokaryotic and fungal but not protistan community compositions from the three soils were distinct. Compositional turnover rates were higher for fungi and protists than for prokaryotes and, for all, lower in clay. Conventional tillage had the strongest effect on protist diversity. In co-occurrence networks, most nodes were provided by prokaryotes, but highly connected nodes by predatory protists in the first, and by saprotrophic fungi in the second year. The temporal variation established here can provide insights of what is natural and thus below the limits of concern in detecting adverse effects on the soil microbiome.