Arctic soil microbial communities may shift with increasing temperatures and water availability from climate change. We examined temperature and volumetric liquid water content (VWC) in the upper 80 cm of permafrost-affected soil over 2 years (2018-2019) at the Bayelva monitoring station, Ny & Aring;lesund, Svalbard. We show VWC increases with depth, whereas in situ temperature is more stable vertically, ranging from -5 degrees C to 5 degrees C seasonally. Prokaryotic metagenome-assembled genomes (MAGs) were obtained at 2-4 cm vertical resolution collected while frozen in April 2018 and at 10 cm vertical resolution collected while thawed in September 2019. The most abundant MAGs were Acidobacteriota, Actinomycetota, and Chloroflexota. Actinomycetota and Chloroflexota increase with depth, while Acidobacteriota classes Thermoanaerobaculia Gp7-AA8, Blastocatellia UBA7656, and Vicinamibacteria Vicinamibacterales are found above 6 cm, below 6 cm, and below 20 cm, respectively. All MAGs have diverse carbon-degrading genes, and Actinomycetota and Chloroflexota have autotrophic genes. Genes encoding beta -glucosidase, N-acetyl-beta-D-glucosaminidase, and xylosidase increase with depth, indicating a greater potential for organic matter degradation with higher VWC. Acidobacteriota dominate the top 6 cm with their classes segregating by depth, whereas Actinomycetota and Chloroflexota dominate below similar to 6 cm. This suggests that Acidobacteriota classes adapt to lower VWC at the surface, while Actinomycetota and Chloroflexota persist below 6 cm with higher VWC. This indicates that VWC may be as important as temperature in microbial climate change responses in Arctic mineral soils. Here we describe MAG-based Seqcode type species in the Acidobacteriota, Onstottus arcticum, Onstottus frigus, and Gilichinskyi gelida and in the Actinobacteriota, Mayfieldus profundus.

A total of 256 water samples were collected from the river, precipitation, and permafrost active layer in a typical small alpine catchment during the ablation periods in 2020 and 2021. The results indicated that every water body was alkaline, and the TDS and EC concentrations were in the following order: precipitation Ca2+ & AP; Mg2+ and Na+ + K+ > Mg2+ > Ca2+, respectively; the anion concentration showed the order of SO42 � > Cl- > NO3 . The results revealed that permafrost and river water had similar geochemical compositions. Similar & delta;2H and & delta;18O values were also observed between river and permafrost water. Additionally, the water chemistry of rivers and permafrost revealed that the chemical weathering of carbonate and silicate rocks is an important source of riverine solutes; however, silicate weathering played a more crucial role. Both hydrochemistry and stable isotopes collectively indicated that there was a close hydraulic connectivity between the water content in river and permafrost active layer in the small alpine catchment. Based on the end-member mixing analysis model, the water in permafrost active layer and precipitation accounted for 62% and 38% of the runoff, respectively, indicating that it was dominated by permafrost during the ablation period. The warming and hu-midification of climate tend to facilitate permafrost degradation. Thus, studying the transformation of different water bodies in alpine regions is imperative to provide water resource security and sustainable development in alpine regions.

The ecological carrying capacity (ECC) is a barometer for ecosystem sustainability. Alpine grassland ecosystems are thought to be the most sensitive ecosystems to climate change. Yet, the ECC of alpine grassland is less well understood. This study aims to establish a structural dynamics model that it enables us to capture different states, changes in tendency, as well as major driving variables of alpine grassland ECC. The results showed that the active layer thickness had a significant adverse effect on ECC (p = 0.05), while precipitation, air temperature, net primary productivity (NPP) had a significant positive effect on ECC (p = 0.01). And anthropogenic factors like fenced pasture, warm shed area, sown grassland area, and livestock density also caused an increase in ECC (p = 0.05). The ECC of alpine grassland displayed an increasing trend on the Qinghai-Tibetan Plateau (QTP). The mean contributions of active layer thickness, NPP, precipitation, and air temperature to the ECC were - 10.0% (p = 0.05), 52.1% (p = 0.01), 17.0% (p = 0.01), and 12.0% (p = 0.01), respectively. From 1980 through 2013, the average annual growth of ECC was 9.1%. The sensitivity of the grassland ECC to major climate variables fluctuated, with periods of high and low sensitivity recorded. On a geographical scale, the Tibet Autonomous Region had higher levels of sensitivity to change, with larger fluctuations, in comparison with Qinghai Province. These findings could provide an important basis for effective adaptation of alpine ecosystem to climate change.

Large quantities of organic matter are stored in frozen soils (permafrost) within the Qinghai-Tibetan Plateau (QTP). The most of QTP regions in particular have experienced significant warming and wetting over the past 50 years, and this warming trend is projected to intensify in the future. Such climate change will likely alter the soil freeze-thaw pattern in permafrost active layer and toward significant greenhouse gas nitrous oxide (N2O) release. However, the interaction effect of warming and altered soil moisture on N2O emission during freezing and thawing is unclear. Here, we used simulation experiments to test how changes in N2O flux relate to different thawing temperatures (T-5-5 degrees C, T-10-10 degrees C, and T-20-20 degrees C) and soil volumetric water contents (VWCs, W-15-15%, W-30-30%, and W-45-45%) under 165 F-T cycles in topsoil (0-20 cm) of an alpine meadow with discontinuous permafrost in the QTP. First, in contrast to the prevailing view, soil moisture but not thawing temperature dominated the large N2O pulses during F-T events. The maximum emissions, 1,123.16-5,849.54 mu g m(-2) h(-1), appeared in the range of soil VWC from 17% to 38%. However, the mean N2O fluxes had no significant difference between different thawing temperatures when soil was dry or waterlogged. Second, in medium soil moisture, low thawing temperature is more able to promote soil N2O emission than high temperature. For example, the peak value (5,849.54 mu g m(-2) h(-1)) and cumulative emissions (366.6 mg m(-2)) of (WT5)-T-30 treatment were five times and two to four times higher than (WT10)-T-30 and (WT20)-T-30, respectively. Third, during long-term freeze-thaw cycles, the patterns of cumulative N2O emissions were related to soil moisture. treatments; on the contrary, the cumulative emissions of W-45 treatments slowly increased until more than 80 cycles. Finally, long-term freeze-thaw cycles could improve nitrogen availability, prolong N2O release time, and increase N2O cumulative emission in permafrost active layer. Particularly, the high emission was concentrated in the first 27 and 48 cycles in W-15 and W-30, respectively. Overall, our study highlighted that large emissions of N2O in F-T events tend to occur in medium moisture soil at lower thawing temperature; the increased number of F-T cycles may enhance N2O emission and nitrogen mineralization in permafrost active layer.

The freezing-thawing variation of permafrost active layer increases the complexity of rainfall-runoff processes in alpine river basins, Northwest China. And alpine meadow is the prominent ecosystem in these basins. This study selected a small alpine meadow watershed in the upper reaches of the Shule River Basin, China. We investigated alpine rainfall-runoff processes, as well as impacts of summer thaw depth of active layer, soil temperature and moisture variation on streamflow based on in-situ observations from July 2015 to December 2020. Some hy-drologic parameters or indices were calculated using statistical methods, and impacts of permafrost change on river runoff were assessed using the variable infiltration capacity model (VIC). In the alpine meadow, surface soil (0-10 cm depth) of the active layer starts to freeze in mid-October each year, and begins to thaw in early April. Also, the deeper soil (70-80 cm depth) of the active layer starts to freeze in late October, and begins to thaw in late June. Moisture content in shallow soils fluctuates regularly, whereas deeper soils are more stable, and their response to rainstorms is negligible. During active layer thawing, the moisture content increases with soil depth. In the alpine meadow, vertical infiltration only occurred in soils up to 40 cm deep, and lateral flow occurred in 0-20 and 60-80 cm deep soils at current rainfall intensity. Summer runoff ratios were 0.06-0.31, and runoff floods show lags of 9.5-23.0 h following the rainfall event in the study area. The freeze-thaw process also significantly impacts runoff regression coefficients, which were 0.0088-0.0654 per hour. Recession coefficient decrease negatively correlates with active layer thawing depth in summer and autumn. Alpine river basin permafrost can effectively increase peak discharge and reduce low flow. These findings are highly significant for rainfall-runoff conversion research in alpine areas of inland rivers.

This article discusses the properties and occurrence of an active layer (AL) in the near-surface of the lithosphere in glacial and periglacial environments. This layer shows a seasonal variability in temperature, as a result of the climate. The AL, as classically understood, seasonally thaws and freezes, while in glacial environments it usually only reaches 0 degrees C. The definition of AL is currently not consistent with the definition of permafrost, even though both concepts usually appear linked. For these terms to be comparable, both should be defined based on temperature variability and not exclusively on phase change. Thus, the AL would be described not only as the upper of perennially frozen ground presenting seasonal thaw-freeze cycles (# 1) but as a layer presenting a seasonal variation in temperature (# 2). Classical active layer can be thawed to a depth of approximately 2-8 cm, the thickest AL reaches over 20 m. In the particularly favorable conditions AL might be completely absent with the permafrost beginning at the ground surface. In glacial and sub-marine permafrost environments, the AL includes a layer of liquid water that seasonally accompanies the permafrost. Glaciers and ice sheets are usually devoid of the classical AL. In both cases, the AL is usually horizontal, but in specific terrains such as sea shore cliffs or karst environments, the AL may have a vertical course and may even be reversed. Both AL and permafrost are common in other frozen bodies in the solar system, differing mainly in their thermal character.

The distribution of the permafrost in the Tibetan Plateau has dramatically changed due to climate change, expressed as increasing permafrost degradation, thawing depth deepening and disappearance of island permafrost. These changes have serious impacts on the local ecological environment and the stability of engineering infrastructures. Ground penetrating radar (GPR) is used to detect permafrost active layer depth, the upper limit of permafrost and the thawing of permafrost with the season's changes. Due to the influence of complex structure in the permafrost layer, it is difficult to effectively characterize the accurate structure within the permafrost on the radar profile. In order to get the high resolution GPR profile in the Tibetan Plateau, the reverse time migration (RTM) imaging method was applied to GPR real data. In this paper, RTM algorithm is proven to be correct through the groove's model of forward modeling data. In the Beiluhe region, the imaging result of GPR RTM profiles show that the RTM of GPR makes use of diffracted energy to properly position the reflections caused by the gravels, pebbles, cobbles and small discontinuities. It can accurately determine the depth of the active layer bottom interface in the migration section. In order to prove the accuracy of interpretation results of real data RTM section, we set up the three dielectric constant models based on the real data RTM profiles and geological information, and obtained the model data RTM profiles, which can prove the accuracy of interpretation results of three-line RTM profiles. The results of three-line RTM bears great significance for the study of complex structure and freezing and thawing process of permafrost at the Beiluhe region on the Tibetan Plateau.

This study investigated the CO2 exchange over a 10-year period (2005-2014) inside and above a larch-dominant forest in the central Lena river basin, eastern Siberia. A wet-soil condition, such as that found in the active layer (seasonally thawed soil layer of upper permafrost), containing unusually high soil water close to saturation and partial surface waterlogging, was prolonged during the warm season of 2005-2009. In later years, the soil layer closer to the ground surface became dry (similar to 10% volumetric water content), although the deeper part remained relatively wet (similar to 30%). We quantitatively compared the whole forest and the understory CO2 exchanges to detect the separate effects of excessive soil waters on the overstory and understory vegetation. The conventional light and temperature response functions for half-hourly CO2 fluxes, that is, the net ecosystem exchange of daytime and night-time, respectively, were applicable to the understory observations. Comparison of the fitting parameters of the light response function at two levels revealed a smaller maximum net ecosystem exchange (NEE) under light saturation with a steep response under weak light conditions for the understory. The CO2 exchanges at the understory increased from the wet-soil period to the drying soil period by 46% (1.3 g C m(-2) d(-1)) of gross primary production (GPP) and 29% (1.2 g C m(-2) d(-1)) of ecosystem respiration (ER), while no trend was found in the ecosystem scale fluxes. These increases were due to an increasing understory biomass, changes in plentiful light and soil water in the inside-canopy environments, and enhanced turbulent mixing. The decline in the larch contribution could be compensated for by the understory growth and the remaining wetness of the active layer, which indicated that the interactions between the larch and the understory supported the stability of carbon cycles in this forest ecosystem.

Currently, the community lacks capabilities to assess and monitor landscape scale permafrost active layer dynamics over large extents. To address this need, we developed a concept of a remote sensing based Soil Inversion Model for regional Permafrost (SIM-P) monitoring. The current SIM-P framework includes a satellite-based soil process model and a soil dielectric model. We are also working on incorporating a radar scattering model for Arctic tundra into the SIM-P framework. A unified soil parameterization scheme was developed to harmonize key soil thermal, hydraulic and dielectric parameters in the soil process and radar models that can be used in the joint soil-radar inversion framework. The soil parameter retrievals of the SIM-P framework include soil organic content (SOC) and active layer thickness (ALT). Initial tests of SIM-P using in-situ soil permittivity observations showed reasonable accuracy in predicting site-level SOC and soil temperature profiles at an Alaska tundra site and ALT in Arctic Alaska. SIM-P will be further tested using airborne P- and L-band radar data collected during NASA's Arctic Boreal Vulnerability Experiment (ABoVE) to evaluate the sensitivity of longwave radar to active layer properties.

Permafrost is one of the key components of terrestrial ecosystem in cold regions. In the context of climate change, few studies have investigated resilience of social ecological system (SER) from the perspective of permafrost that restricts the hydrothermal condition of alpine grassland ecosystem. In this paper, based on the structural dynamics, we developed the numerical model for the SER in the permafrost regions of the source of Yangtze and Yellow Rivers, analyzed the spatial-temporal characteristics and sensitivity of the SER, and estimated the effect of permafrost change on the SER. The results indicate that: 1) the SER has an increasing trend, especially after 1997, which is the joint effect of precipitation, temperature, NPP and ecological conservation projects; 2) the SER shows the spatial feature of high in southeast and low in northwest, which is consistent with the variation trends of high southeast and low northwest for the precipitation, temperature and NPP, and low southeast and high northwest for the altitude; 3) the high sensitive regions of SER to the permafrost change have gradually transited from the island distribution to zonal and planar distribution since 1980, moreover, the sensitive degree has gradually reduced; relatively, the sensitivity has high value in the north and south, and low value in the south and east; 4) the thickness of permafrost active layer shows a highly negative correlation with the SER. The contribution rate of permafrost change to the SER is -4.3%, that is, once the thickness of permafrost active layer increases 1 unit, the SER would decrease 0.04 units.