Despite the extensive research conducted on plant-soil-water interactions, the understanding of the role of plant water sources in different plant successional stages remains limited. In this study, we employed a combination of water isotopes (delta 2H and delta 18O) and leaf delta 13C to investigate water use patterns and leaf water use efficiency (WUE) during the growing season (May to September 2021) in Hailuogou glacier forefronts in China. Our findings revealed that surface soil water and soil nutrient gradually increased during primary succession. Dominant plant species exhibited a preference for upper soil water uptake during the peak leaf out period (June to August), while they relied more on lower soil water sources during the post-leaf out period (May) or senescence (September to October). Furthermore, plants in late successional stages showed higher rates of water uptake from uppermost soil layers. Notably, there was a significant positive correlation between the percentage of water uptake by plants and available soil water content in middle and late stages. Additionally, our results indicated a gradual decrease in WUE with progression through succession, with shallow soil moisture utilization negatively impacting overall WUE across all succession stages. Path analysis further highlighted that surface soil moisture (0- 20 cm) and middle layer nutrient availability (20- 50 cm) played crucial roles in determining WUE. Overall, this researchemphasizes the critical influence of water source selection on plant succession dynamics while elucidating un- derlying mechanisms linking succession with plant water consumption.

Permafrost degradation alters the flow rate, direction, and storage capacity of soil moisture, affecting ecohydrological effects and climate systems, and posing a potential threat to natural and human systems. The most widely distributed permafrost regions are coastal, high-latitudes and high-altitudes (mainly by the Qinghai-Tibet Plateau). Past studies have demonstrated that permafrost degradation in these regions lacks sorting out regional driving factors, assessing cascading effects on the hydrological environment and monitoring methods. To address this, we reviewed the historical research situation and major topics of permafrost degradation from 1990 to 2022. We analyzed the spatio-temporal dynamics and driving mechanism of permafrost degradation. Then, we comprehensively discussed the effects of permafrost degradation on the soil physical structure and hydraulic properties, soil microorganisms and local vegetation, soil evapotranspiration and stream runoff, and integrated ecohydrological effects. Permafrost field site data were then collected from existing findings and methods for direct or indirect monitoring of permafrost changes at different scales. These results revealed that the research on the hydrological effects of permafrost change was mainly centered on the soil. In addition, regional environmental factors driving permafrost degradation were inconsistent mainly in coastal regions influenced by sea level, high-latitude regions influenced by lightning and wildfire, and high-altitude regions influenced by topography. Permafrost degradation promoted horizontal and/or vertical hydrological connectivity, threatening the succession of high latitude vegetation communities and the transition from high altitude grassland to desert ecosystems, causing regional water imbalances would mitigate or amplify the ability of integrated ecohydrological benefits to cope with climate warming. The never-monitored permafrost area was 1.55x106 km2, but the limitations of using data for the same period remained a challenging task for soil moisture monitoring. Finally, future research should enhance the observation of driving factors at the monitoring site and combine remote sensing data, model simulations or numerical simulations, and isotope tracers to predict the future degradation state of deep permafrost effectively. It is expected that this review will guide further quantifying the driving mechanisms of permafrost degradation and the resulting cascading effects.

Soil water content (SWC) and soil temperature (ST) are important indicators of environmental change in permafrost regions. In this study, we conducted soil sampling at 89 locations in the Three Rivers Headwaters Region (TRHR) to investigate the individual and synergistic effects of environmental factors on SWC and ST. We used multivariable regression and random forest modelling to analyse the data. The results show that SWC and ST were higher in the southeast TRHR than in the northwest and higher in surface layers than deeper soil layers. The most important factors affecting SWC in the 0-20 cm and 20-40 cm soil layers were soil bulk density and precipitation, while bulk density was the most important factor in the 40-60 cm layer, and soil bulk density and steppe vegetation were the most important factors in the 60-80 cm layer. For ST, altitude, temperature and slope gradient were the drivers in the 0-20 cm surface layer, while altitude and temperature were the most critical drivers in the 20-40 cm, 40-60 cm and 60-80 cm layers. Overall, bulk density and altitude were the key environmental factors influencing SWC and ST values, respectively. The outcomes of this study provide valuable insights into the environmental factors that impact the SWC and ST in permafrost regions, which can guide decision-making processes for sustainable soil management in the context of climate change.

Although many studies have found that global warming has caused permafrost to thaw, we still lack understanding of the mechanism relating permafrost thawing and ecosystem carbon budgets. To compare the effects of freeze-thaw cycles on the grassland ecosystem carbon budget between a permafrost area (PA) and a non-permafrost area (NPA), we established two carbon dioxide flux towers since 2015 to monitor the net ecosystem exchange by eddy covariance (EC) systems at the site of Nalaikh in PA and Hustai in NPA. The gross primary production (GPP), respiration by ecosystems (Reco), and net ecosystem production (NEP) from 2016 to 2019 were estimated using EddyPro 7 and ToviTM. The result showed that, at the PA and NPA sites, the annual GPP was 686.3 and 654.9 g C m- 2 y-1, Reco was 611.5 and 699.6 g C m- 2 y-1, and NEP was 73.8 and -45.5 g C m-2 y- 1, respectively, which implies that the grassland ecosystem was a carbon sink in the PA but a carbon source in the NPA. Then, the effect of the freeze-thaw cycles on the carbon budget was also analyzed. The NEP in the PA (35.3 g C m-2) was significantly larger than in the NPA (0.3 g C m-2) during the thawing period and, similarly, the NEP in the PA (121.7 g C m-2) was also larger than in the NPA (72.1 g C m-2) during the thawed period, implying significantly larger carbon absorption in the PA than in the NPA during both the thawing and thawed periods. Finally, correlation analysis results revealed that the soil water content (SWC) plays an important role in maintaining the ecosystem carbon budget. The degradation of permafrost might accelerate soil thawing and promote the transfer of soil water, and thus greatly affect the carbon budget of grassland ecosystems in Mongolia.

Vegetation patch patterns, which are used as indicators of state, functionality, and catastrophic changes in the arid ecosystem, have received much attention. However, little is known about the controlling factors and indicators that underlie vegetation patch patterns in the alpine grassland ecosystem. Here, we firstly studied characteristics of vegetation patch patterns with aerial photography by using an unmanned aerial vehicle and evaluated the vegetation patch-size distribution with power law (PL) and truncated power law (TPL) models on the central part of the Qinghai-Tibetan Plateau (QTP). We then investigated the effects of environmental factors and biotic disturbances on vegetation patch patterns. The results showed that (1) there were four typical vegetation patch patterns, i.e. spot, stripe, sheet, and uniform patterns; (2) soil water content and air temperature were major environmental factors affecting vegetation patch patterns; (3) biotic disturbance of plateau pika (Ochotona curzoniae) affected vegetation patch patterns by changing the number, area, and connectivity of vegetation patches; and (4) vegetation patch-size distribution parameters were significantly related to soil hydrothermal variables (P < 0.01). We concluded that the development of alpine vegetation patch patterns was controlled by soil hydrothermal conditions and plateau pika's disturbance. We also proposed that gamma (TPL-PL) (difference between absolute values of slopes of TPL and PL curve fits) could serve as an effective indicator for monitoring alpine grassland conditions, and preventing patchiness was a critical task for the alpine ecosystem management and restoration.

The active layer thickness (ALT) in permafrost regions regulates hydrological cycles, water sustainability, and ecosystem functions in the cryosphere and is extremely sensitive to climate change. Previous studies often focused on the impacts of rising temperature on the ALT, while the roles of soil water content and soil granularity have rarely been investigated. Here, we incorporate alterations of soil water contents in soil thermal properties across various soil granularities and assess spatiotemporal ALT dynamics on the Qinghai-Tibet Plateau (QTP). The regional average ALT on the QTP is projected to be nearly 4 m by 2100. Our results indicate that soil wetting decelerates the active layer thickening in response to warming, while latent heat exerts stronger control on ALTs than thermal conductivity does. Under similar warming conditions, active layers thicken faster in coarse soils than in fine soils. An important ramification of this study is that neglecting soil wetting may cause overestimations of active layer thickening on the QTP.

Permafrost affects soil water and soil temperature regimes; however, its effects on net primary production (NPP) remain unknown. Here, we examined temporal-spatial changes in grassland NPP during 2000-2018 in perma-frost and permafrost-free areas on the Qinghai-Tibetan Plateau using the random forest (RF) and radial basis function artificial neural network (RBF-ANN). Our results indicated that the areas that showed increasing, decreasing, and non-significant trends for NPP accounted for 13.88%, 1.90%, and 84.22% of the permafrost area, respectively. For the permafrost-free areas, these NPP trends accounted for 22.25%, 2.68%, and 75.07% of the permafrost-free area, respectively. The mean NPP in the permafrost regions showed a faster and steadier (1.520 g C/m(2)/yr, p < 0.05) increase than in non-permafrost regions (1.224 g C/m(2)/yr, p < 0.05). The Biome-BGC model confirmed that these spatial NPP patterns could be attributed to differences in soil water and soil temperature between permafrost and permafrost-free areas. Both the soil temperature and soil water content in permafrost sites exhibited relatively lower variance than in permafrost-free sites. Although many factors may be attributed to these patterns, our results suggest that there is a possibility that the relatively stable change in permafrost NPP can be explained by the fact that permafrost can regulate soil water and temperature regimes. Therefore, climate warming can increase NPP in cold regions, and permafrost degradation may destabilize the grassland ecosystem, which may cause NPP values to exhibit greater interannual changes in the future.

The hydrological properties of the active soil layer are the key parameters that regulate soil water-heat-solute migration and alter hydrologic cycles in a permafrost region. To date, much remains unknown about the interaction mechanism between permafrost degradation and eco-hydrological processes in the permafrost regions of the Qinghai-Tibet Plateau (QTP). In this study, the soil texture, soil hydrological properties, the soil moisture status, and the hydrothermal processes were measured and analyzed in different degradation degrees of alpine meadow soils on the QTP. The results showed a close relationship between soil hydrological properties and soil physicochemical properties. Freeze-thaw cycles changed the physicochemical and hydrological properties, that is, frequent freeze-thaw cycles promote to permafrost degradation in terms of soil basis properties of active layer. In addition, vegetation on the ground delayed the degradation of frozen soil. The actual available soil water content (SWC) in the root layer was a key factor in the ecohydrological process. The actual effective SWC in the root layers of different alpine meadows was ranked as follows: non-degraded meadow (NDM) > moderately-degraded meadow (MDM) > seriously degraded meadow (SDM) (1.8-5.0% at NDM and 0.0-4.2% at SDM). In addition, the weak soilpermeability in an SDM intensified the deficiency of the available SWC, thereby increasingthe difficulty of ecological restoration. This study provides a basis for ecological environmental protection in permafrost regions and provides a hydrological process model for cold regions under future climate change scenarios.

Degradation of permafrost with a thin overlying active layer can greatly affect vegetation via changes in the soil water and nutrient regimes within the active layer, while little is known about the presence or absence of such effects in areas with a deep active layer. Here, we selected the northeastern Qinghai-Tibet Plateau as the study area. We examined the vegetation communities and biomass along an active layer thickness (ALT) gradient from 0.6 to 3.5 m. Our results showed that plant cover, below-ground biomass, species richness, and relative sedge cover declined with the deepening active layer, while the evenness, and relative forb cover showed a contrary trend. The vegetation indices and the dissimilarity of vegetation composition exhibited significant changes when the ALT was greater than 2.0 m. The vegetation indices (plant cover, below-ground biomass, evenness index, relative forb cover and relative sedge cover) were closely associated with soil water content, soil pH, texture and nutrient content. Soil water content played a key role in the ALT-vegetation relationship, especially at depths of 30-40 cm. Our results suggest that when the ALT is greater than 2.0 m, the presence of underlying permafrost still benefits vegetation growth via maintaining adequate soil water contents at 30-40 cm depth. Furthermore, the degradation of permafrost may lead to declines of vegetation cover and below-ground biomass with a shift in vegetation species.

A model for predicting shallow depth soil temperatures is important and effective to assess the changes in soil conditions related to global climate change and local disturbances. Shallow-depth soil temperature estimation model in cold region in Alaska is developed based on thermal response using air temperature and shallow-depth soil water content during active layer development period of 160 days from May to October. Among the seven soil temperature measurement sites, data from four sites were used for model development, and the remaining three sites were used for model validation. Near the middle of the seven measurement sites, air temperature is monitored at one location. The proposed model implemented concepts of thermal response and cumulative temperature. Temperatures and soil water contents were measured using automated remote sensing technology. Consequently, it was confirmed that the developed model enables fast and accurate assessment of shallow-depth soil temperature during active soil layer development period.