This study investigated seasonal changes in litter and soil organic carbon contents of deciduous and coniferous forests at two altitudes (500 and 1000 m a.s.l.), which were used as proxies for temperature changes. To this aim, adjacent pine (P500 and P1000) and deciduous forests (downy oak forest at 500 m a.s.l. and beech forest at 1000 m a.s.l., D500 and D1000, respectively) were selected within two areas along the western slope of a calcareous massif of the Apennine chain (central Italy). Periodic sampling was carried out within each site (a total of 19 sampling dates: 6 in autumn, 4 in winter and spring, and 5 in summer), taking each time an aliquot of the upper mineral soil horizon and measuring litter thickness and CO2 emission from the soil. The samples were then analyzed for their content of organic C, total N, water-soluble organic C and N (WEOC and WEN, respectively), and the natural abundance of 13C and 15N. Soil and litter C and N stocks were calculated. The chemical and isotopic data suggested that organic C and N transformations from litter to the upper mineral soil horizon were controlled not only by temperature but also by the quality (i.e. C:N ratio) of the plant material. In particular, the more the temperature decreased, the more the quality of the organic matter would influence the process. This was clearly showed by the greater 13C fractionation from litter to soil organic matter (SOM) in D1000 than in P1000, which would indicate a higher degree of transformation under the same thermal condition of the plant residues from the deciduous forest, which were characterized by a more balanced C:N ratio than the pine litter. However, while at 500 m altitude a significant SOM 13C fractionation and a parallel increase in soil CO2 emissions occurred in the warmer seasons, no seasonal delta 13C variation was observed at 1000 m for both forests, despite the different quality of SOM derived from deciduous and coniferous forests. Our findings suggested that organic C and N transformations from litter to the upper soil mineral horizon were greatly controlled by the quality of the plant residues, whereas soil temperature would seem to be the major driver for the seasonal evolution of SOM. This study, by considering two different vegetation types (deciduous and coniferous), allowed to evaluate the combined interactions between the plant residue quality and temperature in controlling litter and SOM mineralisation/accumulation processes.

The Ore Mountains were historically one of the most polluted areas in Europe, where high sulphur dioxide concentrations and a high level of atmospheric deposition led to a vast decline in Norway spruce stands in the mountain ridge plateau. In this article, we evaluate the trends in the atmospheric deposition load, soil chemistry, tree nutrition, crown defoliation and height increment in a network of twenty research plots monitored for last thirty years in this region. The decrease in sulphur and nitrogen deposition was most pronounced at the end of 1990s. Extreme values of sulphur deposition (100-200 kg.ha-1.year-1) were recorded in throughfall under mature Norway spruce stands in the late 1970s, and after felling of the damaged stand, the deposition levels were comparable to open plot bulk deposition. Nitrogen deposition decreased more slowly compared with sulphur, and a decrease in base cation deposition was observed concurrently. The current deposition load is low and fully comparable to other mountain areas in central Europe. Accordingly, the health of young spruce stands, as assessed by defoliation and height increment, has improved and now corresponds to the Czech national average. On the other hand, no significant changes were observed in the soil chemistry, even though some of the plots were limed. Acidic or strongly acidic soil prevails, often with a deficiency of exchangeable calcium and magnesium in the mineral topsoil, as well as decreases in available phosphorus. This is reflected in the foliage chemistry, where we see an imbalance between a relatively high content of nitrogen and decreasing contents of phosphorus, potassium and calcium. Despite the observed positive trends in air quality and forest health, the nutritional imbalance on acidified soils poses a risk for the future of forest stands in the region.

The central carbon (C) metabolic network is responsible for most of the production of energy and biosynthesis in microorganisms and is therefore key to a mechanistic understanding of microbial life in soil communities. Many upland soil communities have shown a relatively high C flux through the pentose phosphate (PP) or the Entner-Doudoroff (ED) pathway, thought to be related to oxidative damage control. We tested the hypothesis that the metabolic organization of the central C metabolic network differed between two ecosystems, an anoxic marsh soil and oxic upland soil, and would be affected by altering oxygen concentrations. We expected there to be high PP/ED pathway activity under high oxygen concentrations and in oxic soils and low PP/ED activity in reduced oxygen concentrations and in marsh soil. Although we found high PP/ED activity in the upland soil and low activity in the marsh soil, lowering the oxygen concentration for the upland soil did not reduce the relative PP/ED pathway activity as hypothesized, nor did increasing the oxygen concentration in the marsh soil increase the PP/ED pathway activity. We speculate that the high PP/ED activity in the upland soil, even when exposed to low oxygen concentrations, was related to a high demand for NADPH for biosynthesis, thus reflecting higher microbial growth rates in C-rich soils than in C-poor sediments. Further studies are needed to explain the observed metabolic diversity among soil ecosystems and determine whether it is related to microbial growth rates. IMPORTANCE We observed that the organization of the central carbon (C) metabolic processes differed between oxic and anoxic soil. However, we also found that the pentose phosphate pathway/Entner-Doudoroff (PP/ED) pathway activity remained high after reducing the oxygen concentration for the upland soil and did not increase in response to an increase in oxygen concentration in the marsh soil. These observations contradicted the hypothesis that oxidative stress is a main driver for high PP/ED activity in soil communities. We suggest that the high PP/ED activity and NADPH production reflect higher anabolic activities and growth rates in the upland soil compared to the anaerobic marsh soil. A greater understanding of the molecular and biochemical processes in soil communities is needed to develop a mechanistic perspective on microbial activities and their relationship to soil C and nutrient cycling. Such an increased mechanistic perspective is ecologically relevant, given that the central carbon metabolic network is intimately tied to the energy metabolism of microbes, the efficiency of new microbial biomass production, and soil organic matter formation. We observed that the organization of the central carbon (C) metabolic processes differed between oxic and anoxic soil. However, we also found that the pentose phosphate pathway/Entner-Doudoroff (PP/ED) pathway activity remained high after reducing the oxygen concentration for the upland soil and did not increase in response to an increase in oxygen concentration in the marsh soil. These observations contradicted the hypothesis that oxidative stress is a main driver for high PP/ED activity in soil communities. We suggest that the high PP/ED activity and NADPH production reflect higher anabolic activities and growth rates in the upland soil compared to the anaerobic marsh soil. A greater understanding of the molecular and biochemical processes in soil communities is needed to develop a mechanistic perspective on microbial activities and their relationship to soil C and nutrient cycling. Such an increased mechanistic perspective is ecologically relevant, given that the central carbon metabolic network is intimately tied to the energy metabolism of microbes, the efficiency of new microbial biomass production, and soil organic matter formation.

The impact of climate change has become increasingly severe in forests, where droughts and strong winds on the one hand and extreme rainfall events on the other hand can damage forest ecosystems. To mitigate the effects of drought and enhance soil water retention capacity, three types of soil conditioners (SCs), labeled SC_R, SC_CG, and SC_ZZC, were developed as part of the European project ONEforest. All the conditioners are based on Xanthan gum and have different types and amounts of fillers with diverse cellulose fiber lengths. These can offer the potential to optimize the SC characteristics, e.g., water absorption, water retention, and mechanical stability. This paper focuses on the influence of fillers in the SCs on the geotechnical properties of forest soils from Ljubelj in the Alpine part of Slovenia (S1), Catalonia, northeastern Spain (S2), and Heldburg, Germany (S3). The results show an increase of 53% to 100% in the water absorption of treated soil. A less favorable impact of the SCs was found on the drained shear strength and the compressibility. The drained shear strength of untreated forest soils in a saturated state was S1 c ' = 4.4 kPa, phi ' = 33.5 degrees; S2 c ' = 1.4 kPa, phi ' = 30.0 degrees; and S3 c ' = 12 kPa, phi ' = 28.0 degrees. The addition of SCs results in a reduction in the drained shear strength of saturated mixtures. The reduction depends on the dosage of added SC-whether it is a low (L) or a high (H) dosage. For instance, when the soil S1 was treated with a low dosage of the soil conditioner SC_R, it demonstrated a cohesion (c ') of 11 kPa and a friction angle (phi ') of 27.0 degrees. However, increasing the dosage of the SC_R led to a decrease in both the cohesion and the friction angle for the same soil (c ' = 7.7 kPa, phi ' = 25.0 degrees). Additionally, the type of soil conditioner also impacts the drained shear strength. Among the mixtures with a high dosage of the SC_R, SC_CG, or SC_ZZC, those containing the SC_CG with the longest fibers stand out, demonstrating the highest friction angle. Therefore, longer fibers can be a promising component of the SCs to reduce the negative influence of XG on the mechanical properties of treated soils.

Some soil behaviors change significantly as water content varies over time. The particle size distribution of soils has a direct impact on mechanical properties such as soil water content, resistance to dispersion, swelling-shrinkage, fluidity, plasticity, and stickiness. This study was conducted to investigate the usability of Atterberg limits, consistency index and coefficient of linear extensibility (COLE) in the temporal planning of ecosystem restoration activities such as silvicultural interventions, tillage, afforestation, and the construction of forest roads, etc. Surface soil samples were collected from the sections numbered 263, 264, 266, 268, 317, 318, 319, 323, 324,325 and 366 of the degraded forest of the And & imath;r & imath;n forestry operations department. The COLE, liquid limit (LL), plastic limit (PL), plasticity index and consistency index values of soil samples were determined. The LL values ranged from 17.5 to 62.4%, the PL values from 8.2 to 46.8% and the PI values from 6.4 to 15.5. The highest COLE value (0.13) was recorded in the 318, while the lowest COLE value (0.03) was in 325.The LL and PL values have a positive linear relationship with clay and organic matter content. All sections have karstic characteristics, but the mechanical characteristics of the soils differ significantly between the sections. Silvicultural interventions carried out especially in 318, which had the lowest consistency index (Ic = 0.70), and sections 263, 264, 317 and 319, where the consistency index is >2,should take mechanical properties into account, and the planting time intervals should be determined, accordingly for sustainable forestry.

The disturbance that ground-based extraction operations can imply on the forests ecosystem is an issue which demands more attention. Skidding and forwarding are the two most common ground-based extraction systems. While skidding implies to partially or fully dragging the logs on the ground, in forwarding, timber is transported on a deck thus avoiding direct contact with the soil. Generally, skidding is considered to be more impactful than forwarding in relation to the amount of disturbance on forest soil and residual stand. However, the framework depicted in current literature is not so strict. While skidding actually implies a higher level of damage to residual stand, the situation concerning disturbance to forest soil is much more complex. The dissimilarities in the results from various studies on this topic have shown the level of complexity. The lack of research investigating the consequences of the two extraction systems on the overall forest ecosystem is evident. Only a few studies were focused for example on the implications on biodiversity. However, the beneficial effects of best management practices, such as the application of snatch blocks during winching or positioning brush mats on the skid trails/ strip roads to reduce soil compaction, have been clearly demonstrated.

Analysis of climatic conditions for the period of instrumental measurement in Central Yakutia showed three periods with two different mean annual air temperature (MAAT) shifts. These periods were divided into 1930-1987 (base period A), 1988-2006 (period B) and 2007-2018 (period C) timelines. The MAAT during these three periods amounted -10.3, -8.6 and -7.4 degrees C, respectively. Measurement of active layer depth (ALD) of permafrost pale soil under the forest (natural) and arable land (anthropogenic) were carried out during 1990-2018 period. MAAT change for this period affected an early transition of negative temperatures to positive and a later establishment of negative temperatures. Additionally, a shortening of the winter season and an extension of the duration of days with positive temperatures was found. Since the permafrost has a significant impact on soil moisture and thermal regimes, the deepening of ALD plays a negative role for studied soils. An increase in the ALD can cause thawing of underground ice and lead to degradation of the ice-rich permafrost. This thaw process causes a change of the ecological balance and leads to the destruction of natural landscapes, sometimes with a complete or prolonged loss of their biological productivity. During this observation (1990-2018 period) the active layer of permafrost is characterized by high dynamics, depending on climatic parameters such as air temperature, as well as thickness and duration of snow cover. A significant increase in ALD of forest permafrost soils-by 80 cm and 65 cm-on arable land was measured during the observation period (28 years).

The northern regions are experiencing considerable changes in winter climate leading to more frequent warm periods, rain-on-snow events and reduced snow pack diminishing the insulation properties of snow cover and increasing soil frost and freeze-thaw cycles. In this study, we investigated how the lack of snow cover, formation of ice encasement and snow compaction affect the size, structure and activities of soil bacterial and fungal communities. Contrary to our hypotheses, snow manipulation treatments over one winter had limited influence on microbial community structure, bacterial or fungal copy numbers or enzyme activities. However, microbial community structure and activities shifted seasonally among soils sampled before snow melt, in early and late growing season and seemed driven by substrate availability. Bacterial and fungal communities were dominated by stress-resistant taxa such as the orders Acidobacteriales, Chaetothyriales and Helotiales that are likely adapted to adverse winter conditions. This study indicated that microbial communities in acidic northern boreal forest soil may be insensitive to direct effects of changing snow cover. However, in long term, the detrimental effects of increased ice and frost to plant roots may alter plant derived carbon and nutrient pools to the soil likely leading to stronger microbial responses.