Analysis of climatic conditions for the period of instrumental measurement in Central Yakutia showed three periods with two different mean annual air temperature (MAAT) shifts. These periods were divided into 1930-1987 (base period A), 1988-2006 (period B) and 2007-2018 (period C) timelines. The MAAT during these three periods amounted -10.3, -8.6 and -7.4 degrees C, respectively. Measurement of active layer depth (ALD) of permafrost pale soil under the forest (natural) and arable land (anthropogenic) were carried out during 1990-2018 period. MAAT change for this period affected an early transition of negative temperatures to positive and a later establishment of negative temperatures. Additionally, a shortening of the winter season and an extension of the duration of days with positive temperatures was found. Since the permafrost has a significant impact on soil moisture and thermal regimes, the deepening of ALD plays a negative role for studied soils. An increase in the ALD can cause thawing of underground ice and lead to degradation of the ice-rich permafrost. This thaw process causes a change of the ecological balance and leads to the destruction of natural landscapes, sometimes with a complete or prolonged loss of their biological productivity. During this observation (1990-2018 period) the active layer of permafrost is characterized by high dynamics, depending on climatic parameters such as air temperature, as well as thickness and duration of snow cover. A significant increase in ALD of forest permafrost soils-by 80 cm and 65 cm-on arable land was measured during the observation period (28 years).

The northern regions are experiencing considerable changes in winter climate leading to more frequent warm periods, rain-on-snow events and reduced snow pack diminishing the insulation properties of snow cover and increasing soil frost and freeze-thaw cycles. In this study, we investigated how the lack of snow cover, formation of ice encasement and snow compaction affect the size, structure and activities of soil bacterial and fungal communities. Contrary to our hypotheses, snow manipulation treatments over one winter had limited influence on microbial community structure, bacterial or fungal copy numbers or enzyme activities. However, microbial community structure and activities shifted seasonally among soils sampled before snow melt, in early and late growing season and seemed driven by substrate availability. Bacterial and fungal communities were dominated by stress-resistant taxa such as the orders Acidobacteriales, Chaetothyriales and Helotiales that are likely adapted to adverse winter conditions. This study indicated that microbial communities in acidic northern boreal forest soil may be insensitive to direct effects of changing snow cover. However, in long term, the detrimental effects of increased ice and frost to plant roots may alter plant derived carbon and nutrient pools to the soil likely leading to stronger microbial responses.